HS12 Service Manual V3 0_EN (1)

Prévia do material em texto

hybriSpot12 Service Manual V3.EO_EN (17/12/2015) 1/41 
 
 
 
 
SERVICE MANUAL 
 
 
 
 
 
 
 
 
 
 
 
 
 
VITRO S.A 
Calle Luís Fuentes Bejarano. Edificio Nudo Norte (Local 3) 
41020 Sevilla (SPAIN) 
www.vitro.bio 
T. +34 902 366 974 
F. +34 902 366 977 
e-mail: vitro@vitro.bio 
http://www.vitro.bio/
mailto:vitro@vitro.bio
 
 
hybriSpot12 Service Manual 2/41 
 
INDEX 
1 GENERAL DESCRIPTION ....................................................................................................... 3 
1.1 Introduction .......................................................................................................................3 
1.2 Aim ......................................................................................................................................3 
1.3 Rights and Warranties ......................................................................................................3 
1.3.1 Intellectual property ................................................................................................3 
1.3.2 Usage ..........................................................................................................................3 
1.3.3 Documentation and general information ..........................................................4 
1.4 Information and security instructions ............................................................................5 
2 TECHNICAL INFORMATION ................................................................................................. 6 
2.1 Technical data ..................................................................................................................6 
2.2 Minimum requirements for a computer .......................................................................6 
2.3 Serial Number ....................................................................................................................7 
2.4 Special Tools ......................................................................................................................7 
2.5 Recommended Tools.......................................................................................................8 
2.6 Content list .........................................................................................................................9 
2.7 Troubleshooting .............................................................................................................. 10 
3 UNPACKAGING ................................................................................................................. 12 
4 INSTALLATION ..................................................................................................................... 13 
5 HYBRISOFT INSTALLATION .................................................................................................. 13 
6 USB BARCODE READER CONFIGURATION ....................................................................... 13 
7 MECHANICAL SYSTEM ....................................................................................................... 13 
7.1 Safety Instructions .......................................................................................................... 13 
7.2 General Description – Spare Parts .............................................................................. 14 
7.2.1 Housing and Covers.............................................................................................. 14 
7.2.2 Reaction Chamber ............................................................................................... 17 
7.2.3 Hydraulic System ................................................................................................... 18 
7.2.4 Electronic System .................................................................................................. 19 
7.3 Access to the Hydraulic Circuit................................................................................... 21 
7.4 Access into the instrument and electronics circuits ............................................... 21 
8 ELECTRONIC SYSTEM .......................................................................................................... 23 
8.1 Cables Colour Codes ................................................................................................... 23 
8.2 General Description ...................................................................................................... 23 
8.2.1 Boards interconnections ...................................................................................... 24 
8.2.2 Power Circuit Board .............................................................................................. 25 
8.2.3 Main Connections ................................................................................................. 26 
8.3 Display Circuit Board ..................................................................................................... 30 
8.4 Service Port and Program Port .................................................................................... 31 
8.5 DC Power Connector ................................................................................................... 32 
8.6 Camera circuit board and Camera Housing .......................................................... 32 
9 HYDRAULIC SYSTEM ........................................................................................................... 33 
8 CONTROL SOFTWARE ......................................................................................................... 34 
8.1 Valves and Pumps Control Area ................................................................................ 34 
8.2 Temperature Control .................................................................................................... 35 
8.3 Commands Menu ......................................................................................................... 37 
8.4 Auto Settings Menu ....................................................................................................... 37 
8.5 Flash Memory Programming........................................................................................ 38 
 
 
hybriSpot12 Service Manual 3/41 
 
1 GENERAL DESCRIPTION 
1.1 Introduction 
HybriSpot 12 is a new semi-automatic analyzer that meets all the requirements 
to perform PCR amplification and later DNA array reverse hybridization on a 
nylon membrane, based on the principle of the DNA-FLOW. 
By means of this technology, hybridization process takes place by passing the 
reagents through the membrane by means of a hydraulic vacuum system that 
causes a vertical liquid flow. This assay allows hybridization to occur in a 
tridimensional environment and improves incubation time from hours to minutes 
and increases sensitivity and specificity. 
HybriSpot 12 is used for the design and automation of in vitro molecular tests in 
the field of infectious disease, cancer as well as genetic diseases among others, 
where quick sensitive and specific results are required. 
HybriSpot 12 is manufactured by Vitro, S.A in our industrial plants located in 
Seville-Spain. 
1.2 Aim 
This manual is a referencing guide for the Technical Service field engineers 
when these procedures are performed: 
 Unpacking. 
 Hardware installation. 
 Technical checkout. 
 Maintenance. 
1.3 Rights and Warranties 
1.3.1 Intellectual property 
All acts that violate the rights of intellectual property inherent to the product as 
a whole or any of its components or parts are prohibited. 
1.3.2 Usage 
This device has been designed to be used in an in vitro diagnosis laboratory. 
We disclaim any liability, in the event that the same or any component thereof 
is subject to misuse and / or use other than for which it was designed. Users are 
the only responsible for the management,use, testing, processes and 
outcomes that occur and are obtained with the instrument, or as an auxiliary 
device. 
 
 
 
hybriSpot12 Service Manual 4/41 
 
The manufacturer of the product or one of the components could not take 
responsibility for defects when the product has been sold or misused in a 
market for which it was not designed for. 
By means of CE marker, the manufacturer indicates that the device complies 
with the requirements laid down in European Directives to be applied. 
1.3.3 Documentation and general information 
The manual supplied with the instrument is owned by Vitro, SA and is provided 
to customers and authorized distributors for its use. Unless prior express written 
consent of Vitro SA authorization, copying, dissemination and distribution- fully 
or partially- is forbidden, in any form and/or mean, including all systems and 
electronic means, the text, images and illustrations contained in the manual. 
The information contained in the manual (data, figures, drawings, technical 
details etc.) represent the current state of scientific knowledge and latest 
technology as we understand it after conducting thorough investigations in this 
field, and should not be considered as warranted as our products. Note this 
depends on the commercial contract between the parties. 
No liability, damages, financial loss or profit is accepted from or relevant to the 
statements contained in the manual. 
The manufacturer reserves the right to change the technical specifications and 
manufacturing processes without notice. Only in this way it is possible to 
continuously improve technology and manufacturing techniques used in our 
products. 
 
 
 
 
 
 
 
 
 
 
 
 
hybriSpot12 Service Manual 5/41 
 
1.4 Information and security instructions 
 
The following safety precautions and regulations must be observed when the 
instrument is used particularly for medical diagnosis and in vitro. The failure to 
follow these instructions can lead to reporting false negative results and the 
incorrect distribution of the samples. 
I. All notes in the Service Manual should be read carefully, paying particular 
attention to the following sections: 
 Installation 
 Disinfection procedures 
II. The installation must follow these reasonable steps to ensure that the 
location where the instrument is installed meets the environmental and 
electromagnetic specifications for the analyzer. Any significant movement 
of the equipment must be performed by qualified personnel. 
III. The user should be informed and trained to perform daily maintenance and 
check to ensure the correct operation of the instrument. 
IV. Carefully, follow the instructions in the Service Manual for the operations to 
perform to turn on and off the hybriSpot 12. 
V. All precautions and recommendations, normally used in the laboratory 
(good laboratory practice standards), must be followed. 
VI. If the laboratory does not have power sources protected, you may need an 
uninterruptible power supply (UPS) to protect and nurture the instrument 
during a power outage. 
VII. Using hybriSpot 12 do not ensure the absence of contamination by 
biological risk, so the instrument should be handled as potentially infectious 
item and the usual precautions for these cases should also be followed. Do 
not eat, drink or smoke while handling the device. 
VIII. To reduce the risk of electric shock: Keep away from open circuits. The 
instrument must be connected to a ground. The instrument must be 
equipped with a three-pole cable for connection to 230V or 115V. 
 
 
hybriSpot12 Service Manual 6/41 
 
2 TECHNICAL INFORMATION 
2.1 Technical data 
 
Nominal Voltage: 100 – 240 V 
Nominal Frequency: 50/60 Hz 
Secondary Fuses: 2 xPTC 
Nominal Power 150 VA 
Dimensions (Length X width x height) (without 
webcam embedding) 
235x230x200mm 
Gross weight (without packaging material) 5 kg 
Gross weight (with packaging material) 6.8 kg 
Operating Temperature Range 15ºC - 32º C 
Operating ability 12 samples per cycle 
Standard Cycle Time 50 min – 12 samples 
Temperature Range 20°C - 60°C (Reaching limit 
temperature takes longer) 
 
 
Heating Rate 
4,5°C/Min. (Measured between 30°- 
40°C with ambient temp. of approx. 
23°C) 
Cooling Rate 2°C/Min. (Measured between 30ª- 
40°C with ambient temp. of approx. 
23°C) 
Temperature Accuracy ± 2°C(worsened at high 
temperatures) 
Temperature Uniformity ± 1,5°C 
Temperature Range During Storage Suggested between +5°C and + 45°C 
Samples Configuration Max.12 operating samples 
Operating Pressure Only tested at atmospheric pressure 
Operating Humidity Suggested between 30% - 70% non-
condensing 
Humidity During Storage Suggested between 30% - 70% non-
condensing 
2.2 Minimum requirements for a computer 
Processor: Intel Core i3 
Memory: 4 Gb 
Hard Drive: 250 Gb 
Operating System: Windows 7 (32/64 bits) 
Windows 8.1 (32/64 bits) 
USB ports free: 1 (as minimum) 
Network Connection: Ethernet 10/100 Mbps – RJ45 
Recommended Display: 16:9 format 
 
 
hybriSpot12 Service Manual 7/41 
 
2.3 Serial Number 
To get the serial number of manufacture of the product, see the nameplate on 
the back of the instrument. 
2.4 Special Tools 
These tools are not provided with the instrument. 
Used for: 
 Firmware updating. 
 Communication with the PC to use the Technical Service Software. 
 Vacuum verification. 
PC Connector cable 
 
Program Port Cable Kit. 
 
Vacuometer 
 
 
 
Vacuum Test Cap 
 
 
 
 
VIT-20001 – PC Connector Cable 
VIT-20002 – Program Port Cable Kit 
VIT-20003 – Vacuometer 
VIT-20004 – Vacuum Test Cap 
 
 
 
hybriSpot12 Service Manual 8/41 
 
2.5 Recommended Tools 
 
These tools are recommended in order to the Technical Service can give 
Support to the instrument. These tools are not supplied as spare parts neither as 
special tools. 
Clean Cutter Pliers 
 
 
Socket Wrench Socket Set 
 
 
Set of Allen Wrenches (Metric) 
 
 
Set of Torx Screwdrivers 
 
 
Set of flat Screwdrivers 
 
 
Set of Philips Screwdrivers 
 
 
Set of Tube Wrenches 
 
 
Pliers 
 
 
Tweezers 
 
 
 
Thread Sealant 
 
 
 
 
hybriSpot12 Service Manual 9/41 
 
Thermal Paste 
 
 
Infrared Thermal Detector 
 
 
 
 
 
2.6 Content list 
Spares included in the hybriSpot12 are: 
hybriSpot 12 
hybriSpot12 equipment 
Clean tube 
Waste tube 
Clean bottle 
Waste bottle 
USB cable 
Power supply 
Power cord 
Orings set 
Grids set 
 
 
hybriSpot12 Service Manual 10/41 
 
2.7 Troubleshooting 
INCIDENCE COMMENTS CHECKS 
POSSIBLE CAUSE OF FAILURE (In 
case failure persists after 
checking) 
RESOLUTION 
The instrument does 
not start 
When the instrument is on (and the rear switch is 
pressed) the display lights should turn on immediately 
showing the firmware version installed. The side fan 
should also turn on. 
Verify that the power supply is functioning 
properly. Green LED lighting should be on. 
Otherwise, check that the outlet has 
power supply and cable A / C is properly 
connected 
Power supply failure 
Replace power 
supply 
Check that the power is properly 
connected to the instrument 
 
PCB or wiring error 
Replace the 
instrument 
The display does not 
work properly 
 
 
It is possible that the instrument is properly powered, but 
due to a failure in the wiring of the display, it is not 
turned on or simply it does not show text.In any case, 
the side fan should be on 
 
Check that the side fan is on 
 
If the fan works properly, it means that 
the equipment is properly powered. 
The failure should be the display 
board. 
Replace the 
instrument. 
The instrument does 
not create a vacuum 
 
There can be several causes. Is the failure is coming from 
the presence of a residue in one of the wells of a row 
that fails, the final user can try to solve it. Other causes 
such as failure of peristaltic pumps, valves, fittings, etc., 
can only be solved by field engineers 
 
Check that there is no waste in the area of 
the o-ring. If the fault persists, it is 
recommended to remove the grids and o-
ring and placing them back 
 
Possible leakage in the hydraulic 
system. 
Replace the 
instrument. Follow the 
instructions for check 
and review the 
Hydraulic System. 
The instrument loses 
fluid (reactive / 
wash) 
 
 
Both in normal operation and during a wash cycle, some 
leaking can be detected by checking the bottom of the 
instrument. More likely, one connector of the peristaltic 
pumps is leaking. 
 
 
 
 
Leaking at hose pump connector. If 
the user has a 2.5mm allen key, he 
can open the maintenance plate 
and check this. In any case, the 
instrument should be replaced. 
 
Replace the 
instrument 
The instrument takes 
too long to reach the 
target temperature 
Typically, for HPV or zoonoses, the highest temperature 
ramps should not take more than 10 minutes to 
complete. This depends, also, on the room temperature. 
Check that the instrument is properly used 
and that the side fan is on. You should also 
check that the computer display changes 
the temperature of the reaction chamber. 
Possible failures: Fan breakdown, 
temperature sensors, PCB… 
Replace the 
instrument 
 
 
hybriSpot12 Service Manual 11/41 
 
INCIDENCE COMMENTS CHECKS 
 
POSSIBLE CAUSE OF FAILURE (In case 
failure persists after checking) 
 
RESOLUTION 
The instrument does 
not indicate when it 
reaches target time / 
temperature 
 
 
When the target temperature/time is reached, the 
instrument buzzes with built- in speaker included in the 
display board in order to alert the user and continue the 
cycle. In case of temperature, the buzz should coincide 
with the green led lighting of the display 
 
 Failure in the display board Replace the instrument 
The LED lighting 
system of the image 
capture system are 
not turned on 
 
With the image capture system connected to the PC 
USB and the switch ON, both diffuser LEDs lighting and 
the red LED signal should be ON 
 
Check that the USB cable 
connections on PC and capture 
system are correct 
Failure on the image capture system 
electronic board 
 
Replace the image 
capture system 
 
hybriSoft does not get 
images from webcam 
If the LEDs work correctly, hybriSoft should receive the 
image from the webcam 
Make sure MS Windows recognizes 
the webcam. 
 
If MS Windows recognizes the camera, 
the failure is in the software. Otherwise, 
there is a failure either in the camera or 
in any other electronic connections. 
 
Replace the image 
capture system 
 
 
 
 
 
hybriSpot12 Service Manual 12/41 
 
3 UNPACKAGING 
 
 Remove the documentation included. 
 Remove packaging waste. 
 
 Remove the plastic protection of the reaction chamber cover and 
image capture system and pick up both items. 
 Remove protection foam. 
 
 Remove the power supply, cables and auxiliary tubes, bottle and 
hybriSpot 
 
 
 
 
hybriSpot12 Service Manual 13/41 
 
4 INSTALLATION 
 Connect hybriSpot 12 to power supply 
 Connect waste and cleaning tubes to the side connectors 
 Connect waste tank to the corresponding tubes. 
5 HYBRISOFT INSTALLATION 
 
Please refer to the hybriSoft_ Installation guide located at Vitro ftp site. 
6 USB BARCODE READER CONFIGURATION 
 
An external USB barcode reader could be used. 
In this case you must assure that the barcode reader has the correct USB PC 
keyboard configuration. 
It is necessary add a CR suffix, Carriage Return, for a properly operation. 
Please refer to the specific manufacturer barcode reader documentation for 
more information about how to configure it. 
7 MECHANICAL SYSTEM 
7.1 Safety Instructions 
 Before carrying out any work on the instrument (or on the device), it 
should be disconnected from the power supply. 
 After repairing, all parts must be connected as originally connected by 
the manufacturer. 
 No power cord should be clamped between the housing and any other 
part. 
 Do not disconnect the LCD panel while the instrument is on. 
 Follow the rules to work with components at risk of electrostatic 
discharge. 
 
 
hybriSpot12 Service Manual 14/41 
 
7.2 General Description – Spare Parts 
 
7.2.1 Housing and Covers 
7.2.1.1 Top Panel 
 
VIT-120036 – Top Panel 
VIT-120044 – Housing Cap Set (Top Caps) 
VIT-120025 – Reaction Chamber 
VIT-120059 – Reaction Chamber Cover 
 
 
 
hybriSpot12 Service Manual 15/41 
 
 
VIT-120030 – O-ring Set 
VIT-120057 – Grids Set 
VIT-120032 – Caps Set 
 
VIT-120026 – Reaction Chamber Cover Gasket 
 
 
hybriSpot12 Service Manual 16/41 
 
7.2.1.2 Display Panel 
 
VIT-120040 – Display Panel 
VIT-120041 – Front Panel 
 
7.2.1.3 Connections & Maintenance rear panel 
 
 
 
hybriSpot12 Service Manual 17/41 
 
VIT-120006 – I/O Switch 
VIT-120007 – DC Power Connector 
VIT-120011 – Service Port Cable 
VIT-120021 – Panel Tube Connection Kit 
VIT-120042 – Connection Rear Panel 
VIT-120043 – Maintenance Rear Panel 
7.2.2 Reaction Chamber 
 
VIT-120012 – Thermoelectric Cold Plate Cooler 
 
VIT-120012 Thermoelectric Cold Plate Cooler 
VIT-120023 Bar Straight Set 
 
 
hybriSpot12 Service Manual 18/41 
 
VIT-120029 Quarter turn fastener receptacle 
 
 
VIT-120026 – Reaction Chamber Gasket 
 
7.2.3 Hydraulic System 
 
VIT-120003 – Miniature Solenoid Valve 
VIT-120018 – Tygon Tube ID1/8” – OD1/4” 
 
 
hybriSpot12 Service Manual 19/41 
 
VIT-120019 – Tygon Tube ID1/16” – OD3/16” 
VIT-120020 – Tygon Tube ID3/32” – OD7/32” 
VIT-120024 – Manifold Set 
VIT-120037 – Bottom Panel 
VIT-120045 – Hydraulic Plate 
VIT-120046 – Hydraulic Base Plate 
VIT-120055 – Peristaltic Pump 1.5x1.0mm 
VIT-120056 – Peristaltic Pump Head 1.5x1.0mm 
7.2.4 Electronic System 
 
 
 
hybriSpot12 Service Manual 20/41 
 
VIT-120004 – Housing Fan 
VIT-120005 – Temperature Sensor 
VIT-120008 – Peltier Fan Cable 
VIT-120009 – Peltier Cable 
VIT-120010 – Display Ribbon Cable 
VIT-120013 – Power Circuit Board 
VIT-120014 – Display Circuit Board 
 
 
VIT-120060 – Web Camera Circuit Board 
 
 
 
 
hybriSpot12 Service Manual 21/41 
 
 
VIT-120033 – Image Capture System Webcam 
VIT-120058 – Image Capture System V2 
7.3 Access to the Hydraulic Circuit 
 To access the hydraulic circuit, remove the five (5) screws of the 
Maintenance Rear Panel and remove it. 
 
 
7.4 Access into the instrument and electronics circuits 
 Remove carefully the top panel 4 Housing Caps with the help of two 
screwdrivers. 
 Insert a screwdriver Phillips nº 2 into the hole of each Housing Caps after 
the snap fit unblock the lid and pull up it. 
 
 
hybriSpot12 Service Manual 22/41 
 
 
 Lift the lid carefully to avoid damaging the connecting pipes between 
pumps, valves and Incubation block: 
 
 
 
 
hybriSpot12 Service Manual 23/41 
 
8 ELECTRONIC SYSTEM 
8.1 Cables Colour Codes 
 
As convention all the cables used in the HS12’s components have a specific colour in order to 
help in a faster identification of each component. 
Type of Component Colour Picture 
Fans Green-Black 
 
Peltier Purple-Black 
 
Valves Yellow-Black 
 
Pumps Orange-Black 
 
Temperature Sensor Red-White-Black 
 
DC Power Red-Black 
 
 
 
8.2 General Description 
 
 
 
hybriSpot12 ServiceManual 24/41 
 
8.2.1 Boards interconnections 
 
 
 
 
hybriSpot12 Service Manual 25/41 
 
8.2.2 Power Circuit Board 
 
 
 
 
hybriSpot12 Service Manual 26/41 
 
8.2.3 Main Connections 
 
 
 
hybriSpot12 Service Manual 27/41 
 
Close-up of the connections of Power Supply & Switch, Peltiers and 
Temperature Sensors: 
 
 
 
hybriSpot12 Service Manual 28/41 
 
Close-up of the connections of the pumps, valves and fans: 
 
 
 
 
hybriSpot12 Service Manual 29/41 
 
Close-up of the location of resettable fuses and control LEDS: 
 
Close-up of the location of LEDs control valves and pumps: 
 
 
 
hybriSpot12 Service Manual 30/41 
 
8.3 Display Circuit Board 
 
 
 
hybriSpot12 Service Manual 31/41 
 
8.4 Service Port and Program Port 
 
VIT-20001 - Service Port Cable (Technical Service Tool) 
VIT-20002 – Program Port Cable Kit (Technical Service Tool) 
 
 
hybriSpot12 Service Manual 32/41 
 
8.5 DC Power Connector 
 
 
 
 
8.6 Camera circuit board and Camera Housing 
 
 
 
 
hybriSpot12 Service Manual 33/41 
 
9 HYDRAULIC SYSTEM 
General Description 
2
1
2
1
2
1
REACTION CHAMBER
VALVE 3 VALVE 2 VALVE 1
PUMP 3 PUMP 2 PUMP 1
WASTE TANK
WASTE CONNECTION
CLEAN/AIR CONNECTION
SUCTION
MANIFOLD
WASTE
MANIFOLD
CLEAN/AIR TANK
CLEAN/AIR CONNECTION
3xTYGON R3630 TUBING
(1/16" ID x 3/32" OD)
CHAMBER TEMPERATURE
SENSOR HOLE
TYGON R3603 TUBING
(1/8" ID x 1/4" OD)
TYGON R3603 TUBING
 (1/8" ID x 1/4" OD)
TYGON R3603 TUBING
 (1/8" ID x 1/4" OD)
TYGON R3603 TUBING
 (1/8" ID x 1/4" OD)
3xTYGON R3630 TUBING
(3/32" ID x 7/32" OD)
3xTYGON R3630 TUBING
(3/32" ID x 7/32" OD)
A1A2B2
B1
C3 C2 C1
D3 D2 D1
C6 C5 C4
D6 D5 D4
Instrument hybriSpot 12
Plan HYDRAULIC DIAGRAM
Author DJ
Versión: V01
Fecha: 04/02/14
 
 
 
hybriSpot12 Service Manual 34/41 
 
8 CONTROL SOFTWARE 
 
The control software, HS12 Monitor, detects automatically the USB port 
connection of hybriSpot 12. In case it not automatically detected, you can 
select the connection port using the drop menu HS12 Port: 
 
8.1 Valves and Pumps Control Area 
 
You can make an independent or full set of control for the valves and pumps. 
To do this, open the Valves / Pump section. 
ON button turns the valve or pump on and OFF button turns it off. There is a 
color code to the right of each valve indicating the status of each component: 
•Valves: 
 Green: Turned off 
 Red: Turned on 
 Blue: Low consumption 
 
 
 
hybriSpot12 Service Manual 35/41 
 
 
 
 
 
 
 
 
 
 
 
•Pumps: 
 Green: Turned off 
 Red: Turned on 
When the valve remains on for long, Duty Cycle Time is activated and low 
consumption mode is powered on to safeguard the integrity of the valve and 
save energy. 
Note that there are two different fans connected to the Valve 4 and Pump 4 
positions. The chassis fan is connected to the Pump 4 position and it is always 
working (This position is always in red color). The peltier fan is connected to the 
Valve 4 position and is controlled directly by software. The ON and OFF buttons 
don’t work in this position. 
8.2 Temperature Control 
The temperature control can be monitored through the Temperature Control 
section. 
 Target Temperature: Target temperature to be reached 
 P, I, D, I Lim Values, Delta Shutdown: Target Parameters (internal use and 
no documentary evidence) 
 Fan Stop Temp (º C): Temperature at which the fan stops working. 
 Fan Stop Time (Seg): Time when the fan stop working when reaching 
“Fan Stop Temp”. 
 Temp Gain, Temp OffSet: Target Parameters (internal use and no 
documentary evidence) 
 
 
 
hybriSpot12 Service Manual 36/41 
 
 
 
There is a visual control to manage if the temperature target value has been 
reached and the control of the fans. To perform the temperature control, press 
the Run Control button and to stop control, press the Stop Control button. 
Temp. Control: 
 Green: Turned off 
 Red: Turned on 
 Blue: Low consumption 
Target Reach: 
 Green: Target temperature unreached 
 Red: Target temperature reached 
Fan On: 
 Green: Peltier Fan is not working. 
 Red: Fan Peltier Fan is working. 
 
 
 
 
hybriSpot12 Service Manual 37/41 
 
8.3 Commands Menu 
 Read Config: read the configuration of the current RAM memory of the 
system. 
 Send Config: Send the current configuration to the RAM memory of the 
system. 
 Write Config in Flash: Write the current configuration from the RAM 
memory to the Flash memory of the system. 
 Reload Flash Config: Refresh the current configuration in the RAM 
memory with the one stored in the Flash Memory of the system. 
 Factory Settings: Set the default parameters. 
 System Reset 
 Stop System: 
 
8.4 Auto Settings Menu 
 
 
 
 
 
hybriSpot12 Service Manual 38/41 
 
 TEC Profile: Tool used for temporary temperature control settings on the 
instrument. It is used as a worksheet in which different steps are added, 
such us objectives temperatures and how long you have to maintain 
that temperature. 
8.5 Flash Memory Programming 
The procedure for programming the Flash memory is as follows: 
Connect the Cable Port Kit Program (VIT-20002) to your computer using the USB 
cable and the program port of the Power Circuit Board. 
 
 Start the STM ST-LINK utility and connect to the device. The Connect to 
the target icon is used. 
 
 
hybriSpot12 Service Manual 39/41 
 
 
 Before programming the instrument, delete the content stored in the 
Flash, pressing the Full Chip Erase button. 
 
• A message will indicate the complete deletion of the memory. 
 
 
hybriSpot12 Service Manual 40/41 
 
 
 Once the deletion process is finished, check that the memory content is 
FFFFFFFF in all entries. 
 
 To program the new firmware version click on the Program Verify- icon 
and a new dialog box will appear. 
 
 
hybriSpot12 Service Manual 41/41 
 
 Press the Browse button to select the file with the new program to install 
and press the Start button to begin programming. 
 
 Once the process is completed, verify that the memory has been 
successfully programmed.