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COBAS INTEGRA 400 plus analyzer 
Cedex Bio HT Analyzer
Host Interface Manual
Version 1.24
Software version 3.6/5.1
 
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
2
Publication information
Revision history
Version Description Mandatory Date
1.24 Approvals for Cedex Bio HT Analyzer rephrased.
Information updated for communication, block code 04, block code 07, 
block code 10, line code 00, line code 11, error codes for line code 96, 
and general configuration.
Line code 56 added.
Part added for extended host interface of Cedex Bio HT Analyzer.
u What is new in publication version 1.24 (10)
No March 2018
1.23 Migration to latest Roche documentation standard. Information limited 
to COBAS INTEGRA 400 plus analyzer and Cedex Bio HT Analyzer.
Information about Cedex Bio HT Analyzer added.
No December 2016
1.22 Maintenance Version. Removed simulator.
Changed files: _lc12, i4_kp.htm, i_featur.htm, i_lc10.htm, i_lc53.htm, 
i_toc.htm
No March 2010
1.21 New files: i_e_rre1.htm, ro_ind.jpg, rr_i400_2.JPG New Collated results 
and Ratio behavior. For I800 Version C, no changes in HIF.
Yes Dec 2008
1.20 New features I400 v3.4 & I 800 9864.b1 Typo line code 54/dead links,
Changed files: i_toc, i_ver,index, i_lc53.htm, i_lc09, i_lc54.htm, i4_kp. 
New files i_lc11, i_lc12, i_lc13, i_lc15, i_lc16, i_bc05, i_bc_06, i_bc_07
Yes May 2006
1.19 Order Date handling problem in line 53/ORA-01840 with version 2.3 
(NT only)
Changed files: i4_kp, i_toc, i_ver, index
Yes Jun. 01 2005
1.18 Flags: Change in the flag 22: added to the list of flags where no result is 
produced
Changed files: i_flags, i_toc, i_ver, index
Yes May. 10 2005
1.17 Instrument setup: Pictures for INTEGRA 400 Plus connectors on Vectra 
VL400MT, VL420MT and EVO 510.
Changed files: i_setup, i4_setup, i_ver, index
Yes Apr. 20 2004
1.16 Correction of incorrect links in the INTEGRA 800 Known issues to 
Workflow adaptation and flowchart, correction of links in Features and 
BC 10.
Changed files: i_bc10, i_toc, i8_kp, i_featur, i_ver
Yes Nov. 5 2003
1.15 Version 2.2 corrections, flags 15 to 18, Checksum issues in I400, I 400 
note for line code 51, Block code 62 new info for I700/800
Yes Aug. 11 2003
1.14 Instrument setup I800 + Known issues index, i_ver, i8_setup, i_lc53, 
i8_kp, i7_kp, i4_kp, i_setup, i_knownp, i_toc, i7_setup
Yes Oct. 18 2002
1.13 i_lc50, i_bc10, i_featur Yes Sept. 5 2001
1.12 i_lc54 Yes Jan. 18 2001
1.11 i_bc90 new for I800, i_blocks, i_lines, i_toc No Dec. 22. 2000
1.1 Final changes for I800: - new: i_i7vsi8, - changed: i_welcom, i_toc, 
i_flags, i_featur, i_lc00, i_lc10, i_lc41, i_lc42, i_lc53, i_lc53, i_lc54, i_lc55, 
i_lc98, appendix, rs232, i_bc02, i_bc61, i_bc62
Yes Nov. 19. 2000
1.04 - i_bc10: Note to qc and cal order - i4_tshoo: new - i_bc19 / i_bc11: 
I400 can order 200 tests
Yes July 2000
1.03 - i_flags: Additional Flag X/C function for I400 V2.0 and I800 - i_lc55: 
Test ID > Test No.
Nov.1999
y Revision history
 
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
 3
Edition notice This publication is for Host Interface programmers and 
Roche field engineers of the 
COBAS INTEGRA 400 plus analyzer and the 
Cedex Bio HT Analyzer. It explains the details of 
communication between a host computer and an 
instrument.Every effort has been made to ensure that all 
the information contained in this publication is correct at 
the time of publishing. However, the manufacturer of this 
product may need to update the publication information 
as output of product surveillance activities, leading to a 
new version of this publication.
Where to find information The User Manual / Operator’s Manual for the 
respective analyzer focuses on operation, maintenance, 
troubleshooting, and basic configuration of the analyzers. 
They also contain important safety information. You must 
read the Safety chapter before operating the analyzer.
The Online Help includes all information of the User 
Manual / Operator’s Manual. In addition it contains all 
error messages and a complete software reference.
!
 
General attention
To avoid incorrect results, ensure that you are familiar 
with the instructions and safety information.
r Pay particular attention to all safety notices.
r Always follow the instructions in this publication.
r Do not use the software in a way that is not described 
in this publication.
r Store all publications in a safe and easily retrievable 
place.
Training Do not carry out operation tasks or maintenance actions 
unless you have received training from Roche 
Diagnostics. Leave tasks that are not described in the 
user documentation to trained Roche Service 
representatives.
1.01 Minor corrections and additional hyper-links No Dec. 1998
1.02 - i_index: SC, Sequence Counter, BCS added - introduc: SC link added 
in error handling - i_lc00: Example modified - i_flag: LC00 link added - 
i_bc04: correction in text (patient result) - introduc: Note added; BCS 
needs fixed field length - i_lc41, i_lc43, i_lc54, i_lc55, i_lc57: Examples; 
leading 0 replaced by leading • - i_lc96, i_lc99: I2 changed to S2 - 
i_flags: leading 0 removed - i_lc53: Ratio Order note inserted - i_bc03: 
Collated Order note inserted - appendix: Additional instrument codes 
(AmpliLink, AmpliPrep and TaqMan) - i_lc10: More detailed 
explanation
No Feb. 1999
1.00 First HTML version, based on Revision 2.1 of the paper manual. Valid 
for Integra SW versions 9561 and 9562.
Oct. 1998
Version Description Mandatory Date
y Revision history
 
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
4
Images The screenshots and hardware images in this publication 
have been added exclusively for illustration purposes. 
Configurable and variable data in screenshots, such as 
tests, results, or path names visible therein must not be 
used for laboratory purposes.
Warranty Any customer modification to the system renders the 
warranty or service agreement null and void.
For conditions of warranty, contact your local sales 
representative or refer to your warranty contract partner.
Always leave software updates to a Roche Service 
representative, or perform such updates with their 
assistance.
Copyright © 1998-2018, F. Hoffmann-La Roche Ltd. All rights 
reserved.
License information The COBAS INTEGRA 400 plus analyzer and the 
Cedex Bio HT Analyzer software are protected by 
contract law, copyright law, and international treaties. The 
COBAS INTEGRA 400 plus analyzer and the 
Cedex Bio HT Analyzer contain a user license between 
F. Hoffmann-La Roche Ltd. and a license holder, and only 
authorized users may access the software and use it. 
Unauthorized use and distribution may result in civil and 
criminal penalties.
Open Source and Commercial Software The COBAS INTEGRA 400 plus analyzer and the 
Cedex Bio HT Analyzer may include components or 
modules of commercial or open-source software. For 
further information on the intellectual property and other 
warnings, as well as licenses pertaining to the software 
programs included in the 
COBAS INTEGRA 400 plus analyzer and the 
Cedex Bio HT Analyzer, refer to the electronic distribution 
included with this product.
This open source and commercial software and the 
COBAS INTEGRA 400 plus analyzer or 
Cedex Bio HT Analyzer as a whole can constitute a device 
regulated in accordance with applicable law. For more 
detailed information, refer to the respective User 
Manual / Operator’s Manual and labeling.
Please note that the respective authorization is no longer 
valid according to the corresponding legislation should 
any unauthorized changes be made to the 
COBAS INTEGRA 400 plus analyzer or 
Cedex Bio HT Analyzer.
 
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Softwareversion 3.6/5.1 · Host Interface Manual · Version 1.24 
 5
Trademarks The following trademarks are acknowledged:
COBAS, COBAS INTEGRA, CEDEX, and LIFE NEEDS 
ANSWERS are trademarks of Roche.
All other trademarks are the property of their respective 
owners.
Feedback Every effort has been made to ensure that this publication 
fulfills the intended use. All feedback on any aspect of 
this publication is welcome and is considered during 
updates. Contact your Roche representative, should you 
have any such feedback.
Contact addresses
Approvals for
COBAS INTEGRA 400 plus analyzer
The COBAS INTEGRA 400 plus analyzer meets the 
requirements laid down in:
Directive 98/79/EC of the European Parliament and of the 
Council of 27 October 1998 on in vitro diagnostic medical 
devices.
Directive 2011/65/EU of the European Parliament and of 
the Council of 8 June 2011 on the restriction of the use of 
certain hazardous substances in electrical and electronic 
equipment.
RoHS compliant from Serial No. 410001 (with ISE module) 
and 510001 (without ISE module).
Compliance with the applicable directive(s) is provided by 
means of the Declaration of Conformity.
The following marks demonstrate compliance:
Roche Diagnostics GmbH
Sandhofer Strasse 116
68305 Mannheim 
Germany
Made in Switzerland
For in vitro diagnostic use.
Complies with the provisions of the applicable EU 
directives. 
 
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
6
Approvals for Cedex Bio HT Analyzer The Cedex Bio HT Analyzer meets the requirements laid 
down in:
• Directive 2014/30/EU of the European Parliament and 
Council of 26 February 2014 relating to 
electromagnetic compatibility (EMC).
• Directive 2014/35/EU of the European Parliament and 
Council of 26 February 2014 relating to electrical 
equipment designed for use within certain voltage 
limits.
• Directive 2011/65/EU of the European Parliament and 
of the Council of 8 June 2011 on the restriction of the 
use of certain hazardous substances in electrical and 
electronic equipment.
Compliance with the applicable directive(s) is provided by 
means of the Declaration of Conformity.
The following marks demonstrate compliance:
Issued by Underwriters Laboratories, Inc. (UL) for 
Canada and the US.C US®
Complies with the provisions of the applicable EU 
directives.
RoHS compliant from Serial No. 610001.
Issued by Underwriters Laboratories, Inc. (UL) for 
Canada and the US.C US®
 
Table of contents 7
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Table of contents
Publication information 2
Table of contents 7
Intended use for 
COBAS INTEGRA 400 plus analyzer 9
Intended use for Cedex Bio HT Analyzer 9
Symbols and abbreviations 9
What is new in publication version 1.24 10
What is new in publication version 1.23 11
Communication
1 About communication
Communication concept 17
Message structure 18
Communication control 21
Error handling 24
Host communication handling 25
Synchronization and sequence counter use 26
2 About host interface features
About host interface modes and features 29
Patient mode 30
Orders 33
Host query mode 40
Result requests 44
Protocol version 50
Reference
3 General codes
ASCII code 55
Control characters 56
Instrument codes 57
4 Block codes
About block codes 61
Idle and synchronization blocks 62
Result blocks 63
Order blocks 69
Patient blocks 74
Multi-configuration service blocks 76
Protocol version blocks 79
Control messages blocks 80
5 Line codes
About line codes 83
List of line codes 84
Messages
6 Flags and error codes
About flags 105
Flag X 106
Flag CALC (result error) 108
Flag QC 110
About error codes 111
List of line code 96 error codes 112
List of line code 99 error codes 114
Extended host interface of 
Cedex Bio HT Analyzer
7 Block codes for extended host interface
About block codes for extended HIF 119
Result blocks 120
Operation settings blocks 126
Service Action Blocks 127
Test status blocks 129
Calibration status blocks 130
Cassette status blocks 131
Multi-configuration service block 132
Message Log blocks 133
Sample order status blocks 134
System status blocks 136
Date and time blocks 137
Serial number blocks 138
8 Line codes for extended host interface
About line codes 141
List of line codes for extended HIF 142
Troubleshooting
9 Troubleshooting
About troubleshooting 175
Enabling host interface tracing 176
Viewing the trace file 177
Known host interface issues 178
Appendices
10 Instrument setup
Setting up the instrument for host 
communication 185
About the RS-232 serial interface 195
Index
Index 201
 
8 Table of contents
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
 
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
 9
Intended use for COBAS INTEGRA 400 plus analyzer
The COBAS INTEGRA 400 plus analyzer is a fully 
automated, computerized chemistry analyzer. It is 
intended for in vitro quantitative or qualitative 
determination of a wide range of analytes in various body 
fluids.
Intended use for Cedex Bio HT Analyzer
The Cedex Bio HT Analyzer is a fully automated, 
computerized analyzer for professional use. It is intended 
for determination of a wide range of analytes in various 
aqueous solutions like cell culture and fermentation 
media.
For use in quality control/manufacturing process only.
Important note The Cedex Bio HT Analyzer is only intended for the 
determination of analytes in aqueous solutions such as 
cell culture and fermentation media and is not intended 
for any kind of in vitro diagnostics applications. Therefore, 
the term "patient" does not apply to the 
Cedex Bio HT Analyzer and does not appear anywhere 
within the software. Instead, all references to "Patient" in 
the COBAS INTEGRA 400 plus analyzer software appear 
as "Sample" in the Cedex Bio HT Analyzer software. The 
Cedex Bio HT Analyzer software does not contain any 
references to "patient", "patient mode", or "patient ID" and 
does not request patient information.
Symbols and abbreviations
Product names Except where the context clearly indicated otherwise, the 
following product names and descriptors are used.
Product name Descriptor
COBAS INTEGRA 400 plus analyzer instrument
Cedex Bio HT Analyzer instrument
Host computer, laboratory information 
system or other computer system 
connected to the instrument
host
y Product names
 
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
10
Symbols used in this publication
Abbreviations The following abbreviations are used.
What is new in publication version 1.24
Approvals for the Cedex Bio HT Analyzer The approvals for the Cedex Bio HT Analyzer were 
rephrased.
u Approvals for Cedex Bio HT Analyzer (6)
Symbol Used for
<LF> All characters between the two angle 
brackets indicate an ASCII control 
character.
 _ Space character (ASCII 20H) that is used as 
a separator between single data fields 
within a data line.
 • Space character (ASCII 20H) that is used as 
a place holder inside a single data fields 
and is part of this data field.
y Symbols used in this publication
Abbreviation Meaning
ASCII American standard code for information 
interchange
BC Block code
BCS Block check sum
CR Carriage return
EOT End of transmission
ETX End of text
LC Line code
LF Line feed (line terminator)
LIS Laboratory information system
MCS Multi-configuration service
SC Sequence counter
SOH Start if header
STX Start of text
y Abbreviations
 
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
 11
Updated informationThe information in the following sections was updated:
u Communication concept (17)
u Example for Message structure (18)
u Block check (20)
u Host communication handling (25)
u Block 04: Patient Results (64)
u Block 07: Patient Results with Lot Information (67)
u Block 10: Order Entry (69)
u Line code 00: Result Data (84)
u Example for Line code 11: Reagent Lot 
Information (89)
u List of line code 96 error codes (112)
u Configuration General > Interface (190)
Added information The following information was added:
u Line Code 56: Sample name (98)
Extended host interface of
Cedex Bio HT Analyzer
The block codes and line codes were added that are 
available if the extended host interface on the 
Cedex Bio HT Analyzer is enabled.
u Extended host interface of 
Cedex Bio HT Analyzer (115)
What is new in publication version 1.23
• The COBAS INTEGRA 400 plus analyzer and 
Cedex Bio HT Analyzer host interface information that 
was available online, was migrated to the latest Roche 
documentation standards.
• Information was limited to COBAS INTEGRA 400 plus 
analyzer and Cedex Bio HT Analyzer.
• Information about the Cedex Bio HT Analyzer has 
been added to this publication.
• No other content changes have been made.
 
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
12
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Communication
1 About communication .......................................................................................... 15
2 About host interface features ............................................................................ 27
 
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
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Table of contents
About communication 1
Before proceeding to set up the instrument, it is useful to 
understand the basics and concepts of the various 
communication elements.
In this chapter 1
Communication concept . . . . . . . . . . . . . . . . . . . . . . . . 17
Message structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Block header . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Block data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Block check. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Communication control . . . . . . . . . . . . . . . . . . . . . . . . . 21
Parity check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Flow control (hardware/software handshake) . . . 22
Sequence counter and block check sum . . . . . . . 22
Check of block and line composition . . . . . . . . . . 23
Receive timeout . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Error handling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Host communication handling . . . . . . . . . . . . . . . . . . . 25
Synchronization and sequence counter use. . . . . . . . 26
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
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Table of contents
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About communication 17
Communication concept
The communication is a data communication based on a 
request/response dialog, whereby the host delivers the 
requests and the instrument provides responses. 
Following a request from the host, the instrument will 
transmit a specific response message.
Assuming there are no transmission errors and the 
message can be handled correctly, the instrument will 
respond with a positive acknowledgment message.
If an error occurs for any reason, the instrument will 
respond with a negative acknowledgment message.
If the host does not receive a response upon a request 
within a certain time, a timeout should be activated.
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
18 Message structure
Message structure
All messages are based on the same block structure. The 
number of lines within a block can vary.
q Information inside the message is in ASCII code.
ASCII codes from 0 to 31 and 127 to 255 are not allowed 
within data fields.
Example Result response from an instrument:
In this section
Block header (19)
Block data (19)
Block check (20)
Block header <SOH><LF>
09_COBAS�.INTEGRA..._04<LF>
(1 line)
Block data <STX><LF>
53_Order#.211044711_20/10/93_SER<LF>
55_178<LF>
00_+3.234000E+01_mg/dl_004_023_014_000<LF>
<ETX><LF>
(0 to n lines)
Block check 1<LF>
562<LF>
<EOT><LF>
(0 or 2 lines)
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About communication 19
Block header
The block header contains the following information:
q The number of characters of the block header is 
fixed. If the instrument identifier is not used in its full 
length, digits left over have to be filled with spaces.
Block data
Block data contains the main information. The size of this 
part can vary.
q oDepending on the block, the block data has 0 to n data 
lines.
o The length of the data fields is fixed. Therefore unused 
or partially used data fields have to be filled out with 
spaces.
o Some of the lines contain optional data fields. If any of 
the optional data fields are not used they can be left 
out.
u Related topics
• Block codes (59)
• Line codes (81)
Instrument code Instrument identifier Block code
09_ COBAS•INTEGRA•••_ 04<LF>
Two digit code. It identifies the type of 
instrument.
Sixteen digit alphanumeric. It is helpful in 
case more then one instrument of the 
same type is connected to the host. The 
instrument's identifier can be defined in 
the instrument set-up menu. The host 
may use any 16 digit string. The 
instrument does not refer to it.
Two digit code used to identify the type 
of information which follows. Details on 
the block codes can be found in the 
reference of each instrument.
y Structure of block header
Line code Data fields
53_
55_
00_
Order#211044711_20/10/93_SER04<LF>
178<LF>
+3.234000E+01_mg/dl_004_023_014_000<LF>
Two digit code, used to identify the 
contents of the line, separated by a 
delimiter (space) from the rest of the line
Each line contains 1 to n data fields, separated by a delimiter (space). The amount 
and the size of the data fields depend on the type of line.
y Structure of block data
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
20 Message structure
Block check
The block check contains a sequence counter (SC) and 
the block check sum (BCS).
Instrument code
1<LF>
562<LF>
The length of the block check sum field is fixed. In case of one 
or two digit numbers, the field has to be filled out with leading 
spaces.
y Structure of block check
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About communication 21
Communication control
To maintain safe data communication, the following 
communication control mechanisms are used:
In this section
Parity check (21)
Flow control (hardware/software handshake) (22)
Sequence counter and block check sum (22)
Check of block and line composition (23)
Receive timeout (23)
Parity check
This is the most common method used for detectingerrors on asynchronous interfaces. The number of active 
data bits in each character is counted prior to 
transmission. A parity bit is then added on so that the 
total number of binary 1’s in the complete character 
frame is either odd or even according to the preselection 
in the interface configuration. The receiving device will 
then perform the same inspection.
Any mismatch will generate a parity error: Instruments 
will transmit an error message (block 99). Host systems 
will use the error handling.
u Related topics
• Error handling (24)
Location Controls
RS-232 specific Parity check
Flow control 
(hardware/software 
handshake)
Instrument and host Sequence counter (SC)
Block check sum (BCS)
Instrument specific Check of block and line 
composition
Host specific Time-out control
y Communication control mechanisms
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
22 Communication control
Flow control (hardware/software handshake)
If the instrument or the host is currently not able to 
receive data, it must be able to stop the communication. 
This is done by the flow control.
There are two possibilities, either hardware handshake or 
software handshake.
Suggestion: Software handshake simplifies RS-232 cable 
wiring and host SW handling.
u Related topics
• About the handshake (197)
• About the RS-232 cable (186)
Sequence counter and block check sum
Sequence counter The sequence counter (SC) is a one digit number, which 
alternates between 0 and 1. The SC is used by the host to 
initiate retransmit of data. The host's SC must be 
synchronized to the instrument's SC. Both SCs (host and 
instrument) must be updated simultaneously, otherwise a 
sequence error will occur f instrument will retransmit its 
previous message.
The mathematical presentation appears as follows:
z 1
Block check sum The block check sum (BCS) is a 3-digit, ASCII-coded 
number.
The BCS contains the sum of all values of the message 
starting with <SOH> ... to <SC> (<CR>) <LF> modulo 
1000.
z 2
If any mismatch occurs, the receiving station has to react 
accordingly.
Enable / disable of the SC and BCS can be done in the 
Host Interface Configuration menu (either both BCS 
and SC exist or neither).
q We strongly recommend that you enable the block 
check. Otherwise you cannot be sure that all data are 
correctly transmitted.
SC (inc SC) mod 2=
BCS sum (ord(<SOH>) .. ord(<LF>)) mod 1000=
1 
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About communication 23
Check of block and line composition
Upon any mismatch in block and line composition, 
instruments will transmit an error message (block 99) 
containing the appropriate error code.
Whenever a block or line composition error occurs, this 
indicates that the host software is not completely 
configured as described in this manual and has to be 
modified accordingly.
Instruments never change the block and line composition 
unless the relevant configuration settings have been 
changed. 
u Related topics
• Instrument setup (183)
Receive timeout
An exactly defined response time cannot be guaranteed. 
However, a returned response can be expected within 
60 seconds. As a result, we recommend setting the host’s 
time-out to 180 seconds.
q Time-out control is only foreseen on the host side. 
Use the error handling as described.
Incompletely received messages (without an <EOT> 
termination) will not be acknowledged by the instruments 
(host time-out).
u Related topics
• Error handling (24)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
24 Error handling
Error handling
Whenever the host detects any transmission error, it has 
to re-transmit the previous message keeping the same 
sequence counter as before.
Whenever the instrument detects any transmission error, 
it expects the host to re-transmit the previous message, 
keeping the same sequence counter as before. If you 
want to skip this retransmission, send a synchronization 
request to the instrument, then resume the normal 
communication.
Possible transmission errors:
• Incorrect block check sum, i.e. a corrupted message 
was received
• Parity error was detected
• General error message (block 99) was received
• Time-out occurred
u Related topics
• Synchronization and sequence counter use (26)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About communication 25
Host communication handling
w Suggestion for host communication handling
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
26 Synchronization and sequence counter use
Synchronization and sequence counter use
The protocol only works properly when the sequence 
counters (SC) of both host and instrument have the same 
value. Therefore, whenever the host’s communication 
software is started up, the first step will be to synchronize 
the SCs.
The following procedure is recommended:
w Recommended procedure for sequence counter synchronization
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
 27
Table of contents
About host interface features 2
Communication for the various information items follow 
certain patterns and workflows.
In this chapter 2
About host interface modes and features. . . . . . . . . . 29
Patient mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Patient entry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Patient modification . . . . . . . . . . . . . . . . . . . . . . . . . 32
Patient deletion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Order entry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Order modification (add tests) . . . . . . . . . . . . . . . . 39
Order deletion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Host query mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Result requests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
About result requests. . . . . . . . . . . . . . . . . . . . . . . . 44
Example of result query . . . . . . . . . . . . . . . . . . . . . . 46
Example of unspecific result request . . . . . . . . . . . 48
Example of result request with ratio (collated 
result request) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Protocol version . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
28
Table of contents
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About host interface features 29
About host interface modes and features
u Related topics
• Patient mode (30)
Item Action
Patient(1)
(1) Additional option. When using patient mode, the other 3 actions in 
the table are available.
Entry Modification Deletion .
Order Entry Modification Deletion Host 
query
Results Single 
results
Collated 
results
. .
Status Protocol 
version
. .
y Host interface modes andfeatures and their actions
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
30 Patient mode
Patient mode
Patient information is only required when in patient mode.
q oWhen in patient mode, set up the patient on the 
instrument prior to an order entry.
o When connected to a host, we recommend working in 
sample mode.
WARNING!
 
Incorrect result assignment due to multiple use of 
same patient ID
If you work with patient mode, the patient ID must be 
unique.
r Do not use the same patient ID for more than one 
patient.
In this section
Patient entry (31)
Patient modification (32)
Patient deletion (32)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About host interface features 31
Patient entry
Patient information is required by the instrument only 
when the instrument runs in patient mode.
Patient information is downloaded by means of BC40, 
Patient Entry.
Example
w Example of patient entry information
u Related topics
• Block 40: Patient Entry (74)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
32 Patient mode
Patient modification
Patient information is modified by means of BC42, Patient 
Modification.
u Related topics
• Block 42: Patient Modification (74)
Patient deletion
Patient information is deleted by means of BC41, Patient 
Deletion.
Patient deletion is possible only if there are no orders 
attached to it.
u Related topics
• Block 41: Patient Deletion (74)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About host interface features 33
Orders
Rerun tests Unlike the user interface, the host interface does not 
support order reruns. Reruns are performed by again 
adding the required test to the existing order.
u Order modification (add tests) (39)
In this section
Order entry (33)
Order modification (add tests) (39)
Order deletion (39)
Order entry
Single tests, ratios or profiles can be ordered using BC10, 
Order Entry.
An order entry may contain up to 50 attached tests.
When using patient mode, an order can only be entered if 
the patient information is already available in the 
instrument’s database.
Ratios Ratios, also sometimes called calculated results, are 
results derived form one or more tests combined with a 
formula. For example: 
z 3
Ratios can be ordered in several ways:
• By specific ratio number
• By ordering the individual tests simultaneously in a 
single order, or
• By ordering the tests using a profile
Profiles Profiles are sets of tests that can be ordered using a 
single number. Those sets are programmed on the 
instrument and assigned to a number. A profile can 
contain individual tests or ratios.
Test A Test B
10
---------------+
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
34 Orders
Samples with barcodes If barcodes are used to identify samples, the barcode 
includes the order number and can also include the 
sample type.
When using barcodes, both the parameters Sample 
Position Rack Number and Sample Position Tube Number 
have to be set to 0 (zero) in order entries.
u Line code 54: Order Information (97)
u Configuring barcode setup (192)
Example of order entry communication
w Example of order entry communication
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About host interface features 35
Example of order entry with individual test
numbers
w Example of order entry with individual test numbers
Example of order entry with ratio number q Because there are two ratios tied to the two 
individual tests in the particular case of the test HbA1c, 
ordering one ratio will have the side effect of 
automatically ordering the second one. 
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
36 Orders
w Example of order entry with ratio number
Example of order entry with profile number q Because of the remark in the order by ratio number 
above, in this particular case, the profile should contain 
the two individual tests and not the two ratios. Otherwise 
each test and ratio will be ordered two times
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About host interface features 37
w Example of order entry with profile number
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
38 Orders
Example of unspecific order request
w Example of unspecific order request
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About host interface features 39
Order modification (add tests)
Adding tests to orders which already exist on the 
instrument is done by means of an Order Entry (BC10) 
without the Order Information line (LC54).
u Related topics
• Block 10: Order Entry (69)
• Line code 54: Order Information (97)
Order deletion
Orders are deleted by means of BC11, Order Deletion.
Order deletion is possible if
• The order either has not yet been started or
• The order is finished and all results are accepted
u Related topics
• Block 11: Order Deletion (70)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
40 Host query mode
Host query mode
Host query mode is the most convenient way to work with 
laboratory systems. It allows the user to put samples on 
any instrument at any position on any sample rack. It 
optimizes laboratory workflow, particularly when the 
laboratory has more than one instrument.
A barcoded sample is placed in an instrument. The 
instrument reads the barcode and waits for a host 
request. The instrument then sends a list of any on-board 
samples lacking order information.
Due to the request/response principle, the host must 
transmit an MCS request (BC60) in order to receive the 
instrument response containing sample information. To 
ensure an optimal host query function, the host system 
has to transmit this request periodically, e.g. one request 
every 30 seconds.
Following an MCS request, a complete list of all pending 
sample tubes lacking test orders is transmitted to the host 
system (all tubes are listed within the block 62).
Every entry in this list, i.e. every reported pending sample 
tube, requires its own order entry block (block 10).
The host system should ignore pending sample tube 
information if it does not have associated order 
information available. The tubes will be listed with every 
instrument response to a host MCS request until they are 
removed from the instrument.
To reactivate the host query for a sample (in order to 
allow reruns), the host should always transmit an Order 
Deletion block (Block 11) as soon as it has received all 
results belonging to a specificorder.
We recommend using the following procedure and timing 
when using host query:
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About host interface features 41
w Recommended procedure and timing when using host query
q Requests for samples without order lead to extensive 
database access.
Depending on the size of the database and the type of 
request this may result in poor instrument performance, 
i.e. keep the host’s request intervals as long as possible. 
(e.g. 60 seconds).
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
42 Host query mode
Example of host query communication
w Example of host query communication
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About host interface features 43
w Example of host query communication (continued)
...and so on.
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
44 Result requests
Result requests
In this section
About result requests (44)
Example of result query (46)
Example of unspecific result request (48)
Example of result request with ratio (collated result 
request) (49)
About result requests
Due to the request/response principle, results are 
transmitted to the host only upon the receipt of a Result 
Request (BC09).
COBAS INTEGRA 400 plus analyzer and 
Cedex Bio HT Analyzer allow requesting single results or 
collated results. The BC09/LC10 thus contains a result 
type selector.
q We strongly advise against the use of collated 
results, depending on the database size or fragmentation, 
we cannot guaranty the instrument performance.
No specific result can be requested.
Only accepted results will be transmitted. (Auto accept 
can be configured in Configuration > Processing 
> Result Handling Table).
Usually each result will be transmitted only once. To re-
transmitted a result:
• On the instrument user interface, change to the result 
workbench.
• Select the required results with the mouse.
• Select Retransmit in the Process menu.
We recommend using the following procedure and timing 
for the result request loop:
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About host interface features 45
w Recommended procedure and timing for the result request loop
q Result requests lead to extensive database access.
Depending on the size of the database and the type of 
request this may result in poor instrument performance, 
i.e.keep the host’s request intervals as long as possible. 
(e.g. 60 seconds)
We strongly recommend sending an Order Deletion block 
(BC11) to the instrument as soon as all results associated 
with an order have been transmitted to the host. This 
provides several advantages:
• Re-entered samples will trigger the host query again
• The Order and the Result screen will show only data 
of current interest.
• The instrument’s data station performance will be 
improved.
• No manual data maintenance is required on the 
instrument.
By using this procedure, Order and Result screens are 
cleared. All data are deleted.
u Related topics
• Block 09: Result Request (68)
• Block 10: Order Entry (69)
• Block 11: Order Deletion (70)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
46 Result requests
Example of result query 
w Example of result query communication
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About host interface features 47
w Example of result query communication (continued)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
48 Result requests
w Example of result query communication (continued)
Example of unspecific result request
w Example of unspecific result request
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
About host interface features 49
Example of result request with ratio (collated result 
request)
q The HBA1C used in this example consists of two 
different ratios. This is the reason why the two primary 
tests produce two calculated results.
w Example of result request with ratio (collated result request)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
50 Protocol version
Protocol version
The Protocol Version request (BC92) is used to get the 
Version # of the HIF Protocol.
u Related topics
• Block 92: Protocol Version Request (79)
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Reference
3 General codes.......................................................................................................... 53
4 Block codes .............................................................................................................. 59
5 Line codes................................................................................................................. 81
 
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
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Table of contents
General codes 3
All messages are based on the same block structure, 
which is made up of different entities, called codes.
In this chapter 3
ASCII code. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Control characters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Instrument codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
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Table of contents
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
General codes 55
ASCII code
The following table shows the seven-bit ASCII code in 
accordance with US standard ANSI X3.4 for the 
interchange of information among communication 
devices. The first 32 values are reserved for control codes.
w ASCII code
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
56 Control characters
Control characters
The following control characters are used for the 
communication protocol between an instrument and a 
host computer.
Hex. value Dec. value Description
<SOH> 01H 01 Start of header
<STX> 02H 02 Start of text
<ETX> 03H 03 End of text
<EOT> 04H 04 End of transmission
<LF> 0AH 10 Line feed
<CR> 0DH 13 Carriage return
<Space> 20H 32 Space
<DC1> 11H 17 XON
<DC3> 13H 19 XOFF
y Control characters
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General codes 57
Instrument codes
The instrument code for the 
COBAS INTEGRA 400 plus analyzer is “14”.
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58 Instrument codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
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Table of contents
Block codes 4
Block codes form part of the block header of a message. 
They inform about the type of information that is 
transmitted in the message block.
In this chapter 4
About block codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Idle and synchronization blocks . . . . . . . . . . . . . . . . . . 62
Block 00: Idle Block / Sync Block. . . . . . . . . . . . . . 62
Result blocks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Block 02: Calibration Result . . . . . . . . . . . . . . . . . . 63
Block 03: Control Result . . . . . . . . . . . . . . . . . . . . . 64
Block 04: Patient Results . . . . . . . . . . . . . . . . . . . . . 64
Block 05: Calibration Result with Lot Information 65
Block 06: Control Result with Lot Information . . . 66
Block 07: Patient Results with Lot Information . . . 67
Block 08: Result Request Response. . . . . . . . . . . . 67
Block 09: Result Request . . . . . . . . . . . . . . . . . . . . . 68
Order blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Block 10: Order Entry . . . . . . . . . . . . . . . . . . . . . . . . 69
Block 11: Order Deletion . . . . . . . . . . . . . . . . . . . . . 70
Block 19: Order Manipulation Response. . . . . . . . 71
Response to an order entry or test addition . . 71
Response to an order or test deletion . . . . . . . 72
Patient blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Block 40: Patient Entry . . . . . . . . . . . . . . . . . . . . . . . 74
Block 41: Patient Deletion . . . . . . . . . . . . . . . . . . . . 74
Block 42: Patient Modification . . . . . . . . . . . . . . . . 74
Block 49: Patient Manipulation Response. . . . . . . 75
Multi-configuration service blocks. . . . . . . . . . . . . . . . 76
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Table of contents
Block 60: Multi-Configuration Service (MCS) 
Request (host query) . . . . . . . . . . . . . . . . . . . . . . . . 76
Block 61: Slot Configuration . . . . . . . . . . . . . . . . . . 76
Block 62: Sample Tube Information . . . . . . . . . . . . 77
Block 69: Service Request Response. . . . . . . . . . . 78
Protocol version blocks . . . . . . . . . . . . . . . . . . . . . . . . . 79
Block 92: Protocol Version Request . . . . . . . . . . . . 79
Block 93: Protocol Version Data . . . . . . . . . . . . . . . 79
Control messages blocks. . . . . . . . . . . . . . . . . . . . . . . . 80
Block 99: Control Message . . . . . . . . . . . . . . . . . . . 80
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Block codes 61
About block codes
The following is the table of contents for the block code 
chapter. It also shows the request/response behavior for 
host-instrument communication.
Block composition notation
Host (request) Instrument (response)
Block code Request Block code Request
00 Idle Block / Synch Block 00 Idle Block
09
.
Result Request 02 Calibration Result
03 Control Result
04 Patient Result
05 Calibration Result with Lot Information
06 Control Result with Lot Information
07 Patient Result with Lot Information
08 Result Request Response
00 Idle Block, if no result available
10 Order Entry 19 Order Manipulation Response
11 Order Deletion
40 Patient Entry 49 Patient Manipulation Response
41 Patient Deletion
42 Patient Modification
60 Multi Configuration Service Request 61 Slot Configuration
62 Sample Tube Inform.
63 Cal-CS State
69 Service Request Response
90 System Status Request 91 System Status Response
92 Protocol Version Request 93 Protocol Version Data
.- any Host Request 99 Control Message
y List of block codes
Line 
code
Description Iteration
55 Test ID
1 to m(1)
(1) Number of iterations in all lines
05 Access Date Time
16 CALidx
11 Reagent Lot 
Information
02 Control ID
1 to n(2)
(2) Number of iterations in lines 02 and 12
12 Control Lot 
Information
y Notation of block codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
62 Idle and synchronization blocks
Idle and synchronization blocks
In this section
Block 00: Idle Block / Sync Block (62)
Block 00: Idle Block / Sync Block
Block 00 is used:
• During synchronization procedure for connection 
tests 
• As an answer to a result request if no results are 
available
Block 00 has no lines.
Sync block configuration No block check.
Idle block configuration Block check included.
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes 63
Result blocks
In this section
Block 02: Calibration Result (63)
Block 03: Control Result (64)
Block 04: Patient Results (64)
Block 05: Calibration Result with Lot Information (65)
Block 06: Control Result with Lot Information (66)
Block 07: Patient Results with Lot Information (67)
Block 08: Result Request Response (67)
Block 09: Result Request (68)
Block 02: Calibration Result
Block 02 is transmitted to the host if all of the following 
conditions are met:
• After receiving a result request with result type 
selector 01 or 08
• If an accepted and not yet transmitted calibration 
result is available
• If the instrument is configured accordingly
Line 
code
Description Iteration(1)(2)
(1) m = 1 to 6. m is the number of standard rates in line 03. The 
sequence of m iterations must correspond to the sequence of values 
in line 03. In addition, the number of iterations m has to be equal to 
the number of parameters (standard rates) in line 03.
(2) y = 0 or 1. The value of y depends on whether the antigen excess 
(AGE) check is switched ON or OFF. The instrument always transmits 
an AGE rate line regardless of this parameter. If the AGE check was 
not required by the test, this line contains the values initialized by the 
instrument.
55 Test Id
01 Result Time
03 Standard Rates 1 to y+1
04 Calibrate Curve
00 Result Data 1 to m
07 ABS Sample Check 0 to y
y Block 02
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
64 Result blocks
Block 03: Control Result
Block 02 is transmitted to the host if all of the following 
conditions are met:
• After receiving a result request
• If an accepted and not yet transmitted control result is 
available
• If the instrument is configured accordingly
Results collated by test ID Result type selector 01 or 09 is used.
• m = 1 (one Test ID per result block)
• n = 1 to 6 
q Results of different control orders are not collated.
Results collated by control ID Result type selector 06 is used.
• m = 1 to # of available tests (m is the number of tests 
with not yet transmitted QC results)
• n = 1 (one control ID per result block)
Block 04: Patient Results
Block 04 is transmitted to the host if all of the following 
conditions are met:
• After receiving a result request block 09
• If an accepted and not yet transmitted patient result is 
available
Line 
code
Description Iteration
55 Test ID
1to m
01 Result Time
02 Control ID
1 to n
00 Result Data
y Block 03
Line 
code
Description Iteration(1)(2)
(1) n = 1. If result type selector 01 or 07 is used (single result)
(2) n = 1 to 50. If result type selector 03 or 04 is used (results collated 
by order number)
53 Order ID
56 Sample Name 0 to 1
55 Test ID
1 to n
00 Result Data
y Block 04
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes 65
Block 05: Calibration Result with Lot Information
Block 05 is transmitted to the host if all of the following 
conditions are met:
• After receiving a result request with result type 
selector 11 or 18
• If an accepted and not yet transmitted calibration 
result is available
• If the instrument is configured accordingly
Line 
code
Description Iteration(1)(2)
(1) m = 1 to 6. m is the number of standard rates in line 03. The 
sequence of m iterations must correspond to the sequence of values 
in line 03. In addition, the number of iterations m has to be equal to 
the number of parameters (standard rates) in line 03.
(2) y = 0 or 1. The value of y depends on whether the antigen excess 
(AGE) check is switched ON or OFF. The instrument always transmits 
an AGE rate line regardless of this parameter. If the AGE check was 
not required by the test, this line contains the values initialized by the 
instrument.
55 Test ID
15 Access Date Time
16 Result Indexes
03 Standard Rates 1 to y+1
04 Calibrate Curve
13 Calibrator Lot Information
11 Reagent Lot Information
00 Result Data 1 to m
07 ABS Sample Check 0 to y
y Block 5
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
66 Result blocks
Block 06: Control Result with Lot Information
Block 06 is transmitted to the host if all of the following 
conditions are met:
• After receiving a result request with result type 
selector 11 or 19
• If an accepted and not yet transmitted control result is 
available
• If the instrument is configured accordingly
Results always collated by test ID Result type selector 11 or 19 is used.
• m = 1 (one test ID per result block)
• n = 1 to 6
q Results of different control orders are not collated.
Line 
code
Description Iteration
55 Test ID
1 to m
15 Access Date Time
16 Result Indexes
11 Reagent Lot 
Information
02 Control ID
1 to n
12 Control Lot 
Information
00 Result Data
y Block 06
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes 67
Block 07: Patient Results with Lot Information
Block 07 is transmitted to the host if all of the following 
conditions are met:
• After receiving a result request with result type 
selector 11 or 17
• If an accepted and not yet transmitted patient result is 
available
Block 08: Result Request Response
A possible error of a result request will be answered by 
block 08.
q The message contains the associated error code.
Line 
code
Description Iteration
53 Order ID
56 Sample Name 0 to 1
15 Access Date Time
16 Result Indexes
55 Test ID
11 Reagent Lot Information
00 Result Data
y Block 07
Line 
code
Description
96 Error Message
y Block 08
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
68 Result blocks
Block 09: Result Request
Polling for results is done by means of block 09.
q oOnly accepted and not yet transmitted results will be sent 
to the host.
o Calibration and control results are only transmitted if 
enabled.
Line 
code
Description
10 Result Type Selector
y Block 09
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes 69
Order blocks
In this section
Block 10: Order Entry (69)
Block 11: Order Deletion (70)
Block 19: Order Manipulation Response (71)
Block 10: Order Entry
There are different possibilities for entering orders into 
the instrument work list.
The database set-up defines whether the instrument runs 
in patient mode or sample mode.
No patient information is required if in sample mode.
New sample order
Add tests to an existing sample order
Line 
code
Description Iteration(1)(2)(3)
(1) w = 1 if in patient mode
(2) w = 0 if in patient mode
(3) x = 1 to 200 
50 Patient ID 0 to w
53 Order ID
54 Order Information
56 Sample Name 0 to 1
55 Test ID 1 to x
y Block 10: New sample order
Line 
code
Description Iteration(1)(2)(3)
(1) w = 1 if in patient mode
(2) w = 0 if in patient mode
(3) x = 1 to 200 
50 Patient ID 0 to w
53 Order ID
55 Test ID 1 to x
y Block 10: Add tests to an existing sample order
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
70 Order blocks
WARNING!
 
Incorrect result assignment due to use of same 
patient ID (if in patient mode)
The patient ID has to be unique.
r Do not use the same patient ID for more than one 
patient, otherwise results could be assigned to the 
wrong patient.
Calibration and control order
q Calibration and control orders cannot be ordered 
again until the current one has completed.
Block 11: Order Deletion
Delete sample order Order deletion is possible:
• If the order has not yet been started
• Or if the order is finished and all results are accepted
Delete test order
If several identical test orders exist, the first one that is 
found will be deleted.
q Ratio and profile test orders cannot be deleted via 
the host interface.
Line 
code
Description Iteration
52 Special Order Selection
55 Test ID
y Block 10: Calibration and control order
Line 
code
Description Iteration
53 Order ID
y Block 11: Delete sample order
Line 
code
Description Iteration(1)
(1) x = 1 to 200 
53 Order ID
55 Test ID 1 to x
y Block 11: Delete test order
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes 71
Block 19: Order Manipulation Response
Each request with block code 10, 11 or 12 is answered by 
block 19, the order manipulation response. The block's 
content depends on the request.
The response message structure depends on the success 
of the request and also on the request type. One of the 
following block structures could appear:
Response to an order entry or test addition
Response to a correct order entry or test
addition
Response to an order with a line format error
Response to an incorrect order entry or test
addition with error in patient
Response to an order entry or test addition
with error in sample order
Response to an incorrect order entry with
error in test order
Line 
code
Description Iteration
96 Error Code
y Block 19: Response to a correct order entry or test addition
Line 
code
Description Iteration
96 Error Code
y Block 19: Response to an order with a line format error
Line 
code
Description Iteration
96 Error Code
50 Patient ID
y Block 19: Response to an incorrect order entry or test 
addition with error in patient
Line 
code
Description Iteration
96 Error Code
53 Order ID
54 Order Information 0 to 1
y Block 19: Response to an order entry or test addition with 
error in sample order
Line 
code
Description Iteration(1)
(1) x = 1 to 200
96 Error Code
1 to x
55 Test ID
y Block 19: Response to an incorrect order entry with error in 
test order
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
72 Order blocks
Response to a test additionwith default
sample type, if the previously used sample
type was not a default sample type
Response to an incorrect special order
x = 1 to 200
Response to an order or test deletion
Response to a correct order or test deletion
Response to an order or test deletion with
error in line format
Response to an incorrect order or test
deletion with fault in sample order
Response to an incorrect test deletion
Line 
code
Description Iteration(1)
(1) x = 1 to 200
96 Error Code
1 to x53 Order ID
55 Test ID
y Block 19: Response to a test addition with default sample 
type, if the previously used sample type was not a default 
sample type
Line 
code
Description Iteration
96 Error Code
52 Specific Order Selection
55 Test ID
y Block 19: Response to an incorrect special order
Line 
code
Description Iteration
96 Error Code
y Block 19: Response to a correct order or test deletion
Line 
code
Description Iteration
96 Error Code
y Block 19: Response to an order or test deletion with error in 
line format
Line 
code
Description Iteration
96 Error Code
53 Order ID
y Block 19: Response to an incorrect order or test deletion 
with fault in sample order
Line 
code
Description Iteration
96 Error Code
1 to x
55 Test ID
y Block 19: Response to an incorrect test deletion
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes 73
Response to an order or test deletion with
default sample type if the order’s sample type
was not default sample type
x = 1 to 200
Line 
code
Description Iteration
96 Error Code
1 to x53 Order ID
55 Test ID
y Block 19: Response to an order/test deletion with default 
sample type if the order’s sample type was not default 
sample type
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
74 Patient blocks
Patient blocks
In this section
Block 40: Patient Entry (74)
Block 41: Patient Deletion (74)
Block 42: Patient Modification (74)
Block 49: Patient Manipulation Response (75)
Block 40: Patient Entry
Block 40 is used for entering a new patient.
Block 41: Patient Deletion
Block 41 is used to delete patient information.
q Patient deletion is possible only if there are no 
orders attached to it.
Block 42: Patient Modification
Block 42 is used to change existing patient information
Line 
code
Description
50 Patient ID
51 Patient Information
y Block 40
Line 
code
Description
50 Patient ID
y Block 41
Line 
code
Description
50 Patient ID
51 Patient Information
y Block 42
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes 75
Block 49: Patient Manipulation Response
Each request with blocks 40, 41 or 42 is answered by 
block 49, wherein the contents of block 49 depend on the 
request.
The response message structure depends on the success 
of the request, and also on the request type:
Response to a correct patient entry (BC40),
deletion (BC41),or modification (BC42)
Response to a patient entry, deletion, or
modification having a general error
Response to an incorrect patient entry,
deletion, or modification
Line 
code
Description
96 Error Message
y Block 49
Line 
code
Description
96 Error Message
y Block 49
Line 
code
Description
96 Error Message
50 Patient ID
y Block 49
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
76 Multi-configuration service blocks
Multi-configuration service blocks
In this section
Block 60: Multi-Configuration Service (MCS) Request 
(host query) (76)
Block 61: Slot Configuration (76)
Block 62: Sample Tube Information (77)
Block 69: Service Request Response (78)
Block 60: Multi-Configuration Service (MCS) Request 
(host query)
Block 60 has one line.
Block 60 provides requests for the following functions:
Block 61: Slot Configuration
The reply to an MCS request with service = 0 is block 61 
with the current slot configuration.
The rack numbers of the racks in the slots are sent.
Line 
code
Description
40 Service Selection
y Block 60
0 Slot configuration f response: block 61
1 Onboard sample tubes without orders (host query mode) 
f response: block 62 
This request is used when working in host query mode 
with a barcode on each sample tube.
2 Cal/CS status per test f response: block 63
3 Reserved
4 List of all known sample tubes > response: block 62
Line 
code
Description
41 Slot State
y Block 61
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes 77
Block 62: Sample Tube Information
Block 62 is used either as a response to an MCS request 
with service 1 (onboard sample tubes without orders) or 
as a response to an MCS request with service 4 (list of all 
known sample tubes).
Onboard sample tubes without orders (host
query mode
If the sample tubes are identified by barcodes, the host 
has the possibility to request a list of all onboard sample 
tubes without associated order. This is done by means of 
block 60 with service 1. Block 62 is the associated 
response. It contains one or more lines:
The relevant tube information of every on board sample 
tube that has no associated order is returned to the host 
system.
The host computer should transmit the request 
periodically to the instrument (e.g. every 60 seconds). If 
there are no sample tubes without an associated order on 
the instrument, a block 69 with line 96 (service 61) is 
returned.
Timing and procedure are described in host query.
List of all known sample tubes The host has the possibility of requesting a list of all 
known sample tubes. This is done using block 60 with 
service 4. Block 62 is the associated response. It contains 
one or more lines:
The relevant tube information of all known sample tubes 
is returned to the host system.
The instrument keeps all sample tube information (order 
number, rack, tube position, tube type and possibly 
sample type) until the associated rack is re-inserted into 
the instrument. It then deletes the information and re-
reads the sample tube barcodes.
If there is no sample tube information available, a block 
69 with line 96 (service 61) is returned.
Line 
code
Description Iteration(1)
(1) x = 1 to 105
42 Tube Information 1 to x
y Block 62 Sample tubes without orders
Line 
code
Description Iteration(1)
(1) x = 1 to 14985
42 Tube Information 1 to x
y Block 62 List of all sample tubes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
78 Multi-configuration service blocks
Block 69: Service Request Response
Status response to an MCS request is shown by block 69. 
Block 69 has one line.
Line 
code
Description
96 Error Message
y Block 69
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes 79
Protocol version blocks
In this section
Block 92: Protocol Version Request (79)
Block 93: Protocol Version Data (79)
Block 92: Protocol Version Request
Block 92 is used to interrogate the software version of the 
protocol. Block 92 has no lines
Block 93: Protocol Version Data
Block 93 is the answer to block 92, protocol version 
request. Block 93 contains one line.
Line 
code
Description
98 Protocol Version
y Block 93
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
80 Control messages blocks
Controlmessages blocks
In this section
Block 99: Control Message (80)
Block 99: Control Message
The control message block is transferred upon a general 
error, e.g. transmission disturbed, block check sum error 
or invalid block or line structure build up. Block 99 
contains one line.
Line 
code
Description
99 General Error Code
y Block 99
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
 81
Table of contents
Line codes 5
Line codes form part of the data block of a message. They 
are used to identify the content of the line.
In this chapter 5
About line codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
List of line codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Line code 00: Result Data . . . . . . . . . . . . . . . . . . . . 84
Line code 01: Result Time . . . . . . . . . . . . . . . . . . . . 85
Line code 02: Control ID . . . . . . . . . . . . . . . . . . . . . 85
Line code 03: Standard Rates . . . . . . . . . . . . . . . . . 86
Line code 04: Calibration Curve . . . . . . . . . . . . . . . 86
Line code 07: ABS Sample Check . . . . . . . . . . . . . 87
Line code 10: Result Type Selection. . . . . . . . . . . . 88
Line code 11: Reagent Lot Information . . . . . . . . . 89
Line code 12: Control Lot Information . . . . . . . . . . 89
Line code 13: Calibration Lot Information . . . . . . . 90
Line code 15: Access Date Time . . . . . . . . . . . . . . 90
Line code 16: Result Indexes . . . . . . . . . . . . . . . . . 91
Line code 40: Service Selection . . . . . . . . . . . . . . . 92
Line code 41: Slot State . . . . . . . . . . . . . . . . . . . . . . 92
Line code 42: Tube Information . . . . . . . . . . . . . . . 93
Line code 43: Test State . . . . . . . . . . . . . . . . . . . . . . 93
Line code 44: Cal/CS State . . . . . . . . . . . . . . . . . . . 94
Line code 50: Patient ID. . . . . . . . . . . . . . . . . . . . . . 94
Line code 51: Patient Information. . . . . . . . . . . . . . 95
Line code 52: Special Order Selection. . . . . . . . . . 95
Line code 53: Order ID. . . . . . . . . . . . . . . . . . . . . . . 96
Line code 54: Order Information. . . . . . . . . . . . . . . 97
Line code 55: Test ID . . . . . . . . . . . . . . . . . . . . . . . . 98
Line Code 56: Sample name . . . . . . . . . . . . . . . . . . 98
Line code 57: Priority Modification. . . . . . . . . . . . . 98
Line code 96: Error Code . . . . . . . . . . . . . . . . . . . . . 99
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
82
Table of contents
Line code 98: Protocol Version . . . . . . . . . . . . . . . . 99
Line code 99: General Error Code . . . . . . . . . . . . . 99
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes 83
About line codes
Format code Description
Sx x-character ASCII-string, left adjusted.
Length x is fixed.
Shorter strings have to be filled up with trailing <spaces>.
Example: S15 "Mr.•Evans••••••"
Ix ASCII-formatted x-digit integer value, right adjusted.
Length x is fixed.
Shorter integer values have to be filled up with leading <spaces> or zeros (0).
Example: I5 "00069" or "•••69"
SRx.y ASCII-formatted real value with sign, right adjusted.
x = Length of the whole data record.
y = Number of digits after the decimal point (optional).
Length x is fixed.
Shorter real-values have to be filled up with leading <spaces> or zeros (0).
The decimal point is mandatory.
Example: SR9.3: "-0077.123"
Fx ASCII formatted floating point number, right adjusted.
x = Length of the whole data record.
Length x is fixed.
Shorter real-values have to be filled up with leading <spaces> or zeros (0).
The sign "+" or "-" for the exponent is mandatory.
Decimal point and exponent "E" are mandatory.
Mantissa has exactly one digit before the decimal point in the range 1 to 9.
No <spaces> are allowed between the mantissa and the exponent.
Example: F12: "••••6.11E+33" or F15: "-00007.4321E-02"
D1 Date format 1: DD/MM/YYYY.
10 character ASCII-string.
Example: "04/01/1994"
D2 Date format 2: DD-MMM-YY (used by COBAS Core/Core II).
9 character ASCII-string.
Example: "04-JAN-94"
T1 Time format 1: hh:mm:ss (hh in 24 h format).
8 character ASCII-string.
Example: "22:07:34"
T2 Time format 2: hh:mm (hh in 24 h format) (used by COBAS Core/Core II).
5 character ASCII-string.
Example: "22:07"
y Formats of data fields used in line codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
84 List of line codes
List of line codes
Line code 00: Result Data
Examples Normal result without any flag:
00_•8.694475E+01_U/l•••_•• 
0_••0_••0_••0_•0.000000E+00_•0.000000E+00<LF>
or
">TEST RNG" flag, HIF returns the value and the higher 
range limit:
00_10.694475E+01_U/l•••_•• 
0_••0_•31_••0_•1.500000E+02_•10.00000E+00<LF>
Line code In Line code In
00 Result Data 43 Test State
01 Result Time 44 Cal/CS State
02 Control ID 50 Patient ID
03 Standard Rates 51 Patient Information
04 Calibration Curve 52 Special Order Selection
07 ABS Sample Check 53 Order ID
10 Result Type Selection 54 Order Information
11 Reagent lot information 55 Test ID
12 Control Lot Information 56 Sample name
13 Calibration Lot Information 57 Priority Modification
15 Access Date Time 96 Error Code
16 Result Indexes 97 System Status
40 Service Selection 98 Protocol Version
41 Slot State 99 General Error Code
42 Tube Information
y List of line codes
Description Format Range
Line code 00 
Result F13 +/-9.999999E+99.. +/-1.0E-99
Unit S6 
Flag X I3 0..255
Flag S I3 0 (not used, currently set to 0)
Flag CALC I3 0..255
Flag QC I3 0..255
Range value to flag F13 +/-9.999999E+99.. +/-1.0E-99
Range limit F13 +/-9.999999E+99.. +/-1.0E-99
y Line code 00: Result Data
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes 85
or
"<TEST RNG" flag, HIF returns the value and the lower 
range limit:
00_•3.694475E+01_U/l•••_•• 
0_••0_•30_••0_•1.500000E+02_•5.000000E+00<LF>
q Result will contain the concentration value, even if 
the flag is ">TEST RNG" or "<TEST RNG".
Range limit • Contains the upper limit in the case that we get a 
">TEST RNG" flag.
• Contains the lower limit in the case that we get a 
"<TEST RNG" flag.
• Contains 0.000000E+00 in any other case (flag or not).
Line code 01: Result Time
Example 01_22:07:59<LF>
Line code 02: Control ID
Example 02_ROC-N<LF>
Description Format Range
Line code 01 
Time T1 hh:mm:ss
y Line code 01: Result Time
Description Format Range
Line code 02
QC-name S5
y Line code 02: Control ID
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
86 List of line codes
Line code 03: Standard Rates
Example 03_C_-1.929185E-02_-1.513617E-02<LF>
Line code 04: Calibration Curve
Example 04_•1.000000E+00_-1.513617E-02_-5.716211E-04<LF>
The meaning of parameters 1 to 5 depends on the 
calibration mode used:
• For qualitative calibration, parameter 1 corresponds to 
the cut-off value.
• For quantitative calibration, parameters 1 to 5 
correspond to the curve parameters of the model 
used.
• For ISE calibration, the measured electrode defines 
the parameter. If the Li electrode has been measured, 
parameter 1 contains the Li slope, parameter 2 the Na 
slope and parameter 3 the K slope.
• For all other electrodes, parameter 1 contains the 
associated slope value.
Description Format Range Range description
Compulsory Line code 03
Standard type S1 C Calibration (mean rate)
O Correction(mean rate)
A Cal/Cor antigen excess rate
F13 +/-9.999999E+99 to +/-1.0E-99
Optional Standard rate F13
Standard rate F13
Standard rate F13
Standard rate F13
Standard rate F13
y Line code 03: Standard Rates
Description Format Range
Compulsory Line code 04
Correction factor F13 +/-9.999999E+99 to +/-1.0E-99
Correction offset F13 +/-9.999999E+99 to +/-1.0E-99
Correction parameter 1 F13 +/-9.999999E+99 to +/-1.0E-99
Optional Correction parameter 2 F13
Correction parameter 3 F13
Correction parameter 4 F13
Correction parameter 5 F13
y Line code 04: Calibration Curve
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes 87
Line code 07: ABS Sample Check
Example 07_•0.0000_•0.0000<LF>
Description Format Range
Compulsory Line code 07
Absorbency value:1st wavelength, 1st 
value pair
SR7.4
Absorbency value:2nd wavelength, 
1st value pair
SR7.4
Optional Absorbency value:3rd wavelength, 
2nd value pair
SR7.4
Absorbency value:4th wavelength, 
2nd value pair
SR7.4
Absorbency value:5th wavelength, 
3rd value pair
SR7.4
Absorbency value:6th wavelength, 
3rd value pair
SR7.4
y Line code 07: ABS Sample Check
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
88 List of line codes
Line code 10: Result Type Selection
Example 10_07<LF>
q oOnly accepted and not yet transmitted results are sent to 
the Host.
o Due to instrument performance reasons and to 
guarantee that all results are transmitted, do not use 
collated results requests (i.e., do not use result type 
selector 03, 04 and 05) those are only for backwards 
compatibility.
o Collated results requests also give slightly different 
output from unspecific results requests, with V3.4 and 
higher.
o Calibration and control results are only transmitted if 
enabled.
u See Known host interface issues (178)
u See Line code 53: Order ID (96)
u See Configuring host communication (190)
Description Format Range Range description
Line code 10 
Result type selector 12 01 Unspecific result request: Transmit any available result, i.e.
o Single patient, or
o Single calibration, or
o Control result collated by test ID
02 Reserved
03 Transmit patient results, collated by order ID.Results are only 
transmitted if all tests belonging to an order are finished and 
accepted.
Do not use result 
type selector 03, 
04, 05 or 06
See the note 
below.
04 Transmit patient results, collated by order ID.Blocked tests are 
marked with the test blocked flag (CALC flag 255). Their results 
are sent later as soon as successfully completed along with any 
still-remaining blocked tests.
05 Transmit patient results, collated by order ID.Send all currently 
available results (i.e., all accepted but not yet transmitted 
results)
06 Transmit new control results collated by control ID.
07 Transmit single patient result.
08 Transmit single calibration result.
09 Transmit control results collated by test ID.
11 Transmit any available result with relevant lot(s) information.
17 Transmit any single patient result with reagent lot information.
18 Transmit any single calibration result with reagent & calibration 
info.
19 Transmit any single control result by test ID with reagent & 
control info.
y Line code 10: Result Type Selection
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes 89
Line code 11: Reagent Lot Information
Example 12_68354•••••_30/05/2018<LF>
q oIf no lot information is found or no lot was defined, 
“??????????” will be used and transmitted (without quotes). 
o If no expiration date is given for the lot, “00/00/0000” 
will be used and transmitted (without quotes). 
o If the lot information is shorter than ten characters, the 
string will be filled with spaces on the right side. 
Example, N123A will be N123A • • • • • 
(•) represents a filler space
Line code 12: Control Lot Information
Example 11_N123A•••••_23/04/2006<LF>
q 
o If no lot information is found or no lot was defined, 
“??????????” will be used and transmitted (without 
quotes). 
o If no expiration date is given for the lot, “00/00/0000” 
will be used and transmitted (without quotes). 
o If the lot information is shorter than ten characters, the 
string will be filled with spaces on the right side. 
Example, N123A will be N123A • • • • • 
(•) represents a filler space 
Description Format Range
Line code 11
Reagent lot S10
Reagent expiration date D1 dd/mm/yyyy
y Line code 11: Reagent Lot Information
Description Format Range
Line code 12
Reagent lot S10
Reagent expiration date D1 dd/mm/yyyy
y Line code 12: Control Lot Information
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
90 List of line codes
Line code 13: Calibration Lot Information
Example 13_N123A•••••_23/04/2006<LF>
q 
o If no lot information is found or no lot was defined, 
"??????????" will be used and transmitted (without 
quotes). 
o If no expiration date is given for the lot, "00/00/0000" 
will be used and transmitted (without quotes). 
o If the lot information is shorter than ten characters, the 
string will be filled with spaces on the right side.
Example, N123A will be N123A • • • • • 
(•) represents a filler space
Line code 15: Access Date Time
Example 15_22:07:59_23/04/2006<LF>
q Access date time is the point when the 
measurements are started on the instrument (scheduled 
date time) 
Description Format Range
Line code 13
Calibration lot S10
Calibration expiration date D1 dd/mm/yyyy
y Line code 13: Calibration Lot Information
Description Format Range
Line code 15
Time T1 hh:mm:ss
Date D1 dd/mm/yyyy
y Line code 15: Access Date Time
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes 91
Line code 16: Result Indexes
Example calibration 16_1141901502_••••••••••_•••••••••0<LF>
Example control 16_1141901502_1141901523_•••••••••0<LF>
Example sample 16_1141901502_1141901523_1141901678<LF>
Example sample without control 16_1141901502_•••••••••0_1141901678<LF>
q A calibration result comprises only the calibration 
index with which the calibration itself will be identified.
q A control result is comprised of the calibration index 
(reference to the calibration with which the control was 
calculated) and a control group index, identifying the 
control itself.
q A sample result is comprised of:
o A calibration index (reference to the calibration with 
which the sample result was calculated) 
o If there is an associated control, then there is a control 
group index (reference to the control).
o A sample index, with which the result itself will be 
identified
q A result will have a maximum of three indexes. If one 
index does not exist, it will be replaced with a right-
justified zero. See the examples above.
Description Format Range
Line code 16
Calibration index S10
Control group index S10
Sample result index S10
y Line code 16: Result Indexes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
92 List of line codes
Line code 40: Service Selection
Example 40_1<LF>
Line code 41: Slot State
Example 41_••3_350_••0_••0_••0_784<LF>
Description Format Range Range description
Line code 40
Service request I1 0 Slot configuration
1 Sample tubes without 
orders
3 Reserved
4 List of all sample tubes
y Line code 40: Service Selection
Description Format Range
Line code 41 
Rack number in slot 1 I3 1 to 999
Rack number in slot 2 I3 1 to 999
Rack numberin slot 3 I3 1 to 999
Rack number in slot 4 I3 1 to 999
Rack number in slot 5 I3 1 to 999
Rack number in slot 6 I3 1 to 999
y Line code 41: Slot State
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes 93
Line code 42: Tube Information
Example 42_•21_25_1_AB(012:3456)•••_URI<LF>
Line code 43: Test State
Example 43_•32_1<LF>
Description Format Range Range description
Compulsory Line code 42
Sample position rack number I3 0 Defined by barcode
1 to 999
Sample position tube number I2 0 Defined by barcode
1 to 15
Tube type I1 0 Fixed position tube
1 Barcode tube
Order number S15 Barcode characters
Optional If Sample Types Used is enabled in the database set-up, then sample type is included in line 42.
Sample type S3 Laboratory defined string.
Barcode characters
y Line code 42: Tube Information
Description Format Range Range description
Line code 43 
Test ID I3 1 to 999
Test state I1 0 Test unavailable 
(blocked)
1 Test available
y Line code 43: Test State
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
94 List of line codes
Line code 44: Cal/CS State
Example 44_C_A_10:32:45_30/11/1993<LF>
Line code 50: Patient ID
Example 50_123.12.123.••••<LF>
WARNING!
 
Incorrect result assignment due to use of same 
patient ID (if in patient mode)
Patient ID has to be unique.
r Do not use the same Patient ID for more than one 
patient, otherwise results could be assigned to the 
wrong patient.
u Related topics
• Patient mode (30)
Description Format Range Range description
Line code 44
CAL/CS state type S1 C C = Calibration state
O Correction state
Q Control state
CAL/CS state S1 A Accepted
C Calculated
P Pending
R Reactivated
F Failed (calibrations and corrections only)
Time of last Cal/CS issue T1
Date of last Cal/CS issue D1 01/01/1800 to 31/12/2100
y Line code 44: Cal/CS State
Description Format Range
Line code 50
Patient ID S15
y Line code 50: Patient ID
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes 95
Line code 51: Patient Information
Example 51_07/12/1965_M_Ronald•Reagen•1234•on•the•move!_12
34•New•York••••••••<LF>
q The database set-up function allows the user to 
disable the patient information fields. Nevertheless, on 
the host interface, the birthday, sex and leadertext 1 fields 
have to be transmitted even if the values are unknown.
q The entry for patient leadertext 1 appears in the 
database as patient name. As the field-identifier Name 
cannot be changed and is mandatory for transmission 
anyway, this field should only be used to transmit the 
patient's name.
Line code 52: Special Order Selection
Example 52_03_T<LF>
Description Format Range
Compulsory Line code 51
Birthday D1 01/01/1800 to 31/12/210000/00/0000 if unknown
Sex S1 M, FX if unknown
Patient leadertext 1 S31 <space>s if unknown
Optional Patient leadertext 2 S21
Patient leadertext 3 S21
y Line code 51: Patient Information
Description Format Range Range description
Line code 52
Special order type I2 01 Calibration order
02 Calibration correction order
03 Control order
Attribute S1 I Immediate
y Line code 52: Special Order Selection
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
96 List of line codes
Line code 53: Order ID
Example 53_abcdef987654321_00/00/0000_URI<LF>
q The order number and the sample type may not 
contain <space>s in front of or within the identifier. 
Nevertheless, if the fields are not used in their full length, 
digits left over have to be filled with <space>s.
Predefined sample types There are three generally predefined sample types:
WARNING!
 
r Using a sample specific code instead of “???” (for 
example HEM for hemolysate) will override any 
instrument and test default sample settings and force 
all tests from the order to be executed on such a 
sample type. This has the same effect as selecting a 
specific sample type on the order entry screen of the 
instrument, instead of automatic, which is the default 
in the drop down list.
q Orders:
o If the sample type is not transmitted, the default 
sample type (defined by test or rack definition) is 
used.
Description Format Range
Compulsory Line code 53
Order number S15 Barcode characters
Order date D1 01/01/1800 to 31/12/2100
00/00/0000 (= today)
Optional If sample type is used
Sample type S3 Laboratory defined string barcode characters
y Line code 53: Order ID
??? Sample type determined by instrument’s test 
definitions. Used with order entries and deletions if 
the test-specific sample type should be selected 
automatically.
*** These wild cards are used with order deletions to 
select all sample types.
RTO The instrument sets sample type to RTO for ratio 
results.
RTO is not used when using collated results, but 
still used with unspecific result requests (10 01)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes 97
Results:
o If in Configuration Processing > Result Output 
> Sample Type, the sample type is disabled, no 
sample type information is transmitted to the host.
Ratio orders:
o If the component results of a ratio are measured from 
different sample types, the Order Entry Sample Type 
must be “???”.
u Related topics
• Configuring barcode setup (192)
• Line code 11: Reagent Lot Information (89)
Line code 54: Order Information
Example 54_••4_•4_S_These•are•21•chars•••<LF>
Description Format Range Range description
Compulsory Line code 54
Sample position rack number I3 0 Defined by barcode
1 to 999
Sample position tube number I2 0 Defined by barcode
1 to 15
Order priority S1 A Active
S STAT
Optional Depending on database set-up parameters Leadertext 1 and 2
Sample leadertext 1 S21
Sample leadertext 2 S21
y Line code 54: Order Information
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
98 List of line codes
Line code 55: Test ID
Example 55_••1<LF>
q Profile and ratio handling: Use ratio number to order 
ratio.
Ratio and individual tests results will be delivered.
The available test no., profile IDs and Ratio IDs can be 
found in Configuration > Test Definitions > ID.
Line Code 56: Sample name
Example 56_UrineWithDownregulatedProteinX•••••••••••••••••
•••<LF>
Line code 57: Priority Modification
Example 57_•2<LF>
Description Format Range
Line code 55
Test no. I3 1 to 999
y Line code 55: Test ID
Description Format Range
Line code 56
Sample name S50
y Line code 56: Sample name
Description Format Range Range description
Line code 57
Priority modification type I2 2 Change work list priority to STAT
y Line code 57: Priority Modification
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes 99
Line code 96: Error Code
Example 96_00<LF>
Line code 98: Protocol Version
Example 98_9561<LF>
Line code 99: General Error Code
Example 99_01<LF>
Description Format Range
Line code 96
Error code S2 00 to 99
y Line code 96: Error Code
Description Format Range
Line code 98
Protocol version I4 System SW 9561 = Protocol 
SW 9561
y Line code 98: Protocol Version
Description Format Range
Line code 99
Error code S2 01 to 08
y Line code 99: General Error Code
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual ·Version 1.24 
100 List of line codes
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Messages
6 Flags and error codes.........................................................................................103
 
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
 103
Table of contents
Flags and error codes 6
Flags can be generated for the results of tests on 
samples, calibrations, or quality controls.
In this chapter 6
About flags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Flag X . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Flag CALC (result error). . . . . . . . . . . . . . . . . . . . . . . . . 108
Flag QC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
About error codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
List of line code 96 error codes . . . . . . . . . . . . . . . . . . 112
List of line code 99 error codes . . . . . . . . . . . . . . . . . . 114
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104
Table of contents
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Flags and error codes 105
About flags
The Flags are divided into four groups:
X Conditions under which result was 
generated
S Not yet used, currently set to ’0’
CALC Result error
QC Evaluation of results
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
106 Flag X
Flag X
Flag X provides information about the conditions under 
which a result was generated.
Flag X contains four subgroups. 
• C
• Q
• R
• ^
Flag X is transmitted as 3 ASCII characters which 
represent a 3-digit integer value (value range 0 to 255).
This value represents an 8-bit value. The four subgroups 
are coded within the four two-bit groups of a byte. The 
single flags are binary coded within each two-bit group.
Sub-flag C = (Flag X and 192)/64
Sub-flag Q = (Flag X and 48)/16
Flag X
Decimal 0 ... 255
Binary 1 1 1 1 1 1 1 1
Sub-flag C Q R ^
y Flag notation
Value Name Meaning
0 no flag Result was calculated with an error-free 
calibration.
1 c The result was calculated with an 
erroneous and/or corrected calibration. 
The calibration was already accepted.
2 C The result was calculated with an 
erroneous and/or corrected calibration. 
The calibration was not accepted.
3 p The result was calculated with a 
postponed calibration (I400 V2.0 only).
y Sub Flag C = (Flag X and 192)/64
Value Name Meaning
0 no flag The result was secured with an error-
free control.
1 q The result was secured with an 
erroneous and/or corrected control. The 
control was accepted.
2 Q The result was secured with an 
erroneous and/or corrected control. The 
control was not accepted.
3 Not used
y Sub Flag Q = (Flag X and 48)/16
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Flags and error codes 107
Sub-flag R = (Flag X and 12)/4
Sub-flag ^ = Flag X and 3
Value Name Meaning
0 no flag Result is error-free.
1 r The result is erroneous or corrected, or 
the result of the replicate is erroneous. 
The result of the first measurement and 
the replicate were accepted.
2 R The result is erroneous or corrected, or 
the result of the replicate is erroneous. 
The result of the first measurement and 
the replicate were not accepted.
3 d The result was deleted or all the results 
(with calibration measurements or 
replicates) have been deleted.
y Sub Flag R = (Flag X and 12)/4
Value Name Meaning
0 no flag Result was generated without a 
replicate, sample concentration or 
sample dilution.
1 -> Result was generated with a replicate.
2 v Result was generated after dilution.
3 ^ Result was generated after 
concentration.
y Sub Flag ^ = Flag X and 3
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
108 Flag CALC (result error)
Flag CALC (result error)
Flag CALC provides information about the checks 
performed during the calculation of the result.
q A flag with a higher priority (lower number) 
supersedes all other flags.
Flag value Priority Name Meaning
0 No flag
1 1 HIGH ABS High absorbency
2 2 DARK ERR Dark error
3 3 OUTLIERS Outliers
4 4 FP UNSTAB FP unstable
5 5 POL ERR Polarization error
6 6 LOW SIGNAL Low signal intensity
7 7 NO FLUID No sample
8 8 NO ISECAL No calibrator bottle
9 9 SEG FLUID No end of sample segment detected
10 10 SEG ISECAL No end of standard segment detected
11 11 AIR FLUID Not enough sample / too many bubbles
12 12 AIR ISECAL Not enough standard / too many bubbles
13 13 ISE UNSTAB ISE measurement unstable
14 14 < REAG RNG Low reagent range exceeded
15 15 > REAG RNG High reagent range exceeded
16 16 EP UNSTAB Endpoint unstable
17 17 HIGH ACT High activity (please refer to following note)
18 18 LOW ACT Low activity (please refer to following note)
19 19 CURVE DIR Curve direction
20 20 NON LINEAR Non-linear kinetic
21 21 < KIN READ Insufficient kinetic readings
22 22 HIGH BKG High background noise (please refer to following note)
23 23 > REPL DEV Replicate deviation check failed
24 24 CALC ERROR Calculation error (please refer to following note)
25 25 NON MONO Curve not monotone
26 26 OUT OF RNG Curve / slope out of range
27 27 > STD DEV Standard deviation exceeded
28 28 SOL 1 F DEV Solution 1 factor deviation
29 29 AG EXCESS Antigen excess
30 30 < TEST RNG Lower test range exceeded (please refer to following note)
31 31 > TEST RNG Upper test range exceeded (please refer to following note)
255 33 TEST BLOCKED Used with collated results if a test is blocked (see note)
y List of flag CALC
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Flags and error codes 109
q oGenerally, whenever a flag precludes the calculation of a 
result and the instrument displays “???”, the HIF will always 
transmit the result as -9.999999E+99 or to 9.999999E+99.
o In case of a HIGH ACT, LOW ACT, <TEST RNG, >TEST 
RNG, HIGH BKG, CALC ERROR and TEST BLOCKED 
flag, the transmitted result in line 00 is either set to 
-9.999999E+99 or to 9.999999E+99. The range field is 
set to the appropriate value if applicable.
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
110 Flag QC
Flag QC
Flag QC provides information about those checks which 
indicate whether a result has exceeded a test-specific or 
laboratory-specific limit. For those representations where 
1) is indicated, the value of the exceeded limit is also 
shown (range value in line 00 Result Data).
Flag type Flag value Name Meaning
0 . No flag
Precision control flags 1 R1(2s) 1 value outside ± 2s
2 R1(3s) 1 value outside ± 3s
3 R2(2s) 2 values outside ± 2s
4 R(4s) Difference in two values greater than 4s
5 R4(1s) 4 values outside ± 1s
6 R7v 7 consecutive values decrease
7 R7^ 7 consecutive values increase
8 R7x 7 consecutive values on one side of mean
9 R10x 10 consecutive values on one side of mean
Accuracy control flags 10 > ACC RNG Accuracy outside the permittedrange 1)
Limit control flags 11 > UPLIMIT Value exceeds upper limit 1)
12 < LOWLIMIT Value exceeds lower limit 1)
Quantitative result flags 13 <RR Value below reference range 1)
14 >RR Value above reference range 1)
19 POS Cut off value exceeded 1)
Qualitative result flags 20 POSITIVE Value greater than calibration result
21 < Critical Range Below critical Range
22 > Critical Range Higher then critical Range
23 DC > Delta Check in a value
24 DC > Delta Check in percent
y List of Flag QC
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Flags and error codes 111
About error codes
COBAS INTEGRA 400 plus analyzer and 
Cedex Bio HT Analyzer uses the following error codes:
• Line code 96 error codes
• Line code 99 error codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
112 List of line code 96 error codes
List of line code 96 error codes
Error code Host request Error condition
00 Any Manipulation OK (i.e. no error, manipulation accepted)
11 Patient Modify/Delete Patient ID not found
Order Entry Patient ID not found
12 Patient Entry Patient ID already exists
13 Pat.Entry/Mod./Delete Patient already locked (e.g. by work area etc.) or database 
time-out
Order Entry Database time-out
14 Patient Deletion Patient still has open orders
21 Order Entry (Add Test) Sample ID (Order No./Sample Type/Order Date) not found
Order Deletion Sample ID (Order No./Sample Type/Order Date) not found
Change Work List Priority Attempt to change orders to passive priority
Change Work List Priority No appropriate orders found to change priority
Remove Tests from Order No appropriate tests found in order; No tests removed
22 Order Entry Sample ID already exists; rack/sample position already in use
Order Entry (Add Test) Sample order full (50 test orders already assigned)
Special Order Entry Calibration/Control request already set
23 Any Order Manipulation Order already locked (e.g. by work area, etc.), or
Database time-out
24 Any Order Manipulation Test not defined
25 Order Deletion Order contains not-yet accepted results
33 Result Request Result data already locked
Or: any database error such as:
o Sample type not found in sample type list
o Result has just been deleted
o Database time-out
o etc.
40 Operation Setting No communication with real time software
System Status Request No communication with real time software
Service Action Execution No communication with real time software
41 Operation Setting The current instrument status does not allow the required 
change
Service Action Execution The current instrument status does not allow the requested 
action
52 Cassette Status No cassette on board
55 Sample Order Status No pending tests
56 Sample Order Status Selected Order is not present
58 Test Status Request No tests loaded
59 Test Status Request The specified test is not loaded
61 MCS Pend. Sample Tubes No tubes found
63 MCS Pend. Sample Tubes Sample type not found in sample type list
MCS Calibr./Contr. status No test defined
Any MCS Request Database time-out
65 Calibration Status No test is loaded
66 Service Action Status No Service Action definition is available
y Line code 96 error codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Flags and error codes 113
80 Message Log Request No new message to send
81 Service Action Result Request No new service action result to send
91 Any Host Request General parameter range error (e.g. rack not defined, illegal: 
cup position, sex, dates, etc.)
Unexpected character in number field
Sample type (undefined, *** or ??? are inappropriate)
Any Order Manipulation Sample type (lab number) not found
Error code Host request Error condition
y Line code 96 error codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
114 List of line code 99 error codes
List of line code 99 error codes
Error code Meaning Error condition
01 Block check sum error Transmission error
02 Parity error Transmission error
03 Line too long (>128 characters) Erroneous line configuration, or possibly transmission error if 
block check off
04 Block too long Erroneous block configuration, or possibly transmission error if 
block check off
05 Invalid message configuration Erroneous message configuration, or possibly transmission 
error if block check off
06 Invalid block code Invalid block code, or possibly transmission error if block 
check off
07 Invalid line code Invalid line code, or wrong mode used (patient/sample), or 
possibly transmission error if block check off
08 Line format error Erroneous line configuration, or possibly transmission error if 
block check off
y Line code 99 error codes
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Extended host interface of
Cedex Bio HT Analyzer
 
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
 117
Table of contents
Block codes for extended host 
interface 7
Block codes form part of the block header of a message. 
They inform about the type of information that is 
transmitted in the message block.
If the extended host interface of the 
Cedex Bio HT Analyzer is enabled, the following block 
codes are modified or can additionally be used.
In this chapter 7
About block codes for extended HIF . . . . . . . . . . . . . . 119
Result blocks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Block 02: Calibration Result . . . . . . . . . . . . . . . . . . 120
Block 03: Control Result . . . . . . . . . . . . . . . . . . . . . 121
Block 04: Sample Results . . . . . . . . . . . . . . . . . . . . 122
Block 05: Calibration Result with Lot Information 123
Block 06: Control Result with Lot Information . . . 124
Block 07: Sample Results with Lot Information . . 125
Operation settings blocks . . . . . . . . . . . . . . . . . . . . . . . 126
Block 20: Operation Setting. . . . . . . . . . . . . . . . . . . 126
Block 21: Operation setting response . . . . . . . . . . 126
Service Action Blocks . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Block 25: Service Action Execution . . . . . . . . . . . . 127
Block 26: Service Action response . . . . . . . . . . . . . 127
Block 27: Service Action Result Request. . . . . . . . 127
Block 28: Service Action Result Response . . . . . . 128
Block 29: Failed Service Action Result Response 128
Test status blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
Block 45: Test status request. . . . . . . . . . . . . . . . . . 129
Block 46: Test Status Response . . . . . . . . . . . . . . . 129
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
118
Table of contents
Block 47: Failed Test Status Request . . . . . . . . . . . 129
Calibration status blocks . . . . . . . . . . . . . . . . . . . . . . . . 130
Block 52: Calibration status request. . . . . . . . . . . . 130
Block 54: Calibration Status Response . . . . . . . . . 130
Block 57: Failed Calibration Status Request . . . . . 130
Cassette status blocks . . . . . . . . . . . . . . . . . . . . . . . . . . 131
Block 50: Cassette status request . . . . . . . . . . . . . 131
Block 55: Cassette Status Response .. . . . . . . . . . 131
Block 59: Failed Cassette Status Request . . . . . . . 131
Multi-configuration service block . . . . . . . . . . . . . . . . 132
Block 64: Service Action Status . . . . . . . . . . . . . . . 132
Message Log blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
Block 75: Message Log Request . . . . . . . . . . . . . . 133
Block 76: Message Log Response . . . . . . . . . . . . . 133
Block 77: Failed Message Log Request. . . . . . . . . 133
Sample order status blocks. . . . . . . . . . . . . . . . . . . . . . 134
Block 80: Order Status Request . . . . . . . . . . . . . . . 134
Block 81: Order Status Response . . . . . . . . . . . . . . 134
Block 85: Failed Order Status Request . . . . . . . . . 135
System status blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . 136
Block 90: System status request . . . . . . . . . . . . . . . 136
Block 91: System status response . . . . . . . . . . . . . 136
Date and time blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Block 94: Date and Time request . . . . . . . . . . . . . . 137
Block 95: Date and Time data . . . . . . . . . . . . . . . . . 137
Serial number blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . 138
Block 96: Serial Number Request. . . . . . . . . . . . . . 138
Block 97: Serial Number Response . . . . . . . . . . . . 138
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes for extended host interface 119
About block codes for extended HIF
The following table lists the modified and added block 
codes. It also shows the request/response behavior for 
host-instrument communication.
Block composition notation
Host (request) Instrument (response)
Block code Request Block code Request
02 Calibration Result
03 Control Result
04 Sample Result
05 Calibration Result with Lot Information
06 Control Result with Lot Information
07 Sample Result with Lot Information
20 Operation Setting 21 Operation Setting Response
25 Service Action Execution 26 Service Action Response
27 Service Action Result Request 28 Service Action Result Response
29 Failed Service Action Result Response
45 Test Status Request 46 Test Status Response
47 Failed Test Status Request
52 Calibration Status Request 54 Calibration Status Response
57 Failed Calibration Status Request
50 Cassette Status Request 55 Cassette Status Response
59 Failed Cassette Status Request
64 Automatic service action status
69 Service Request Response
75 Message Log Request 76 Message Log Response
77 Failed Message Log Request
80 Sample Order Status Request 81 Sample Order Status Response
85 Failed Order Status Request
90 System Status Request 91 System Status Response
94 Date and Time Request 95 Date and Time Data
96 Serial Number Request 97 Serial Number Response
y List of block codes
Line 
code
Description Iteration
55 Test ID
1 to m(1)
(1) Number of iterations in all lines
05 Access Date Time
16 CALidx
11 Reagent Lot 
Information
02 Control ID
1 to n(2)
(2) Number of iterations in lines 02 and 12
12 Control Lot 
Information
y Notation of block codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
120 Result blocks
Result blocks
In this section
Block 02: Calibration Result (120)
Block 03: Control Result (121)
Block 04: Sample Results (122)
Block 05: Calibration Result with Lot Information (123)
Block 06: Control Result with Lot Information (124)
Block 07: Sample Results with Lot Information (125)
Block 02: Calibration Result
Block 02 is transmitted to the host if all of the following 
conditions are met:
• After receiving a result request with result type 
selector 01 or 08
• If an accepted and not yet transmitted calibration 
result is available
• If the instrument is configured accordingly
Line code Description Iteration(1)(2)(3)
(1) m = 1 to 6. m is the number of standard rates in line 03. The 
sequence of m iterations must correspond to the sequence of values 
in line 03. In addition, the number of iterations m has to be equal to 
the number of parameters (standard rates) in line 03.
(2) y = 0 or 1. The value of y depends on whether the antigen excess 
(AGE) check is switched ON or OFF. The instrument always transmits 
an AGE rate line regardless of this parameter. If the AGE check was 
not required by the test, this line contains the values initialized by the 
instrument.
(3) n = 1 to 423. The value of n can be different from 1 only if the result 
was measured using an absorbance test (Line 06). There are 423 
possible lines since each line is produced for every replicate (max. 3) 
and every cycle (max 141).
55 Test Id
01 Result Time
03 Standard Rates 1 to y+1
04 Calibrate Curve
00 Result Data
1 to m
06/08/09 Raw Data 1 to n
y Block 02
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes for extended host interface 121
Block 03: Control Result
Block 02 is transmitted to the host if all of the following 
conditions are met:
• After receiving a result request
• If an accepted and not yet transmitted control result is 
available
• If the instrument is configured accordingly
Results collated by test ID Result type selector 01 or 09 is used.
• m = 1 (one Test ID per result block)
• n = 1 to 6 
q Results of different control orders are not collated.
Results collated by control ID Result type selector 06 is used.
• m = 1 to # of available tests (m is the number of tests 
with not yet transmitted QC results)
• n = 1 (one control ID per result block)
Line code Description Iteration(1)
(1) w = 1 to 141. The value of w can be different from 1 only if the result 
was measured using an absorbance test (Line 06). There are 141 
possible lines since each line is produced for every cycle (max 141).
55 Test ID
1 to m
01 Result Time
02 Control ID
1 to n00 Result Data
06/08/09 Raw Data 1 to w
y Block 03
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
122 Result blocks
Block 04: Sample Results
Block 04 is transmitted to the host if all of the following 
conditions are met:
• After receiving a result request block 09
• If an accepted and not yet transmitted sample result is 
available
Line code Description Iteration(1)(2)(3)(4)(5)
(1) n = 1. If result type selector 01 or 07 is used (single result)
(2) n = 1 to 50. If result type selector 03 or 04 is used (results collated 
by order number)
(3) w = 1 to 141. The value of w can be different from 1 only if the result 
was measured using an absorbance test (Line 06). There are 141 
possible lines since each line is produced for every cycle (max 141).
(4) p = 0 to x. The value of x is the number of controls used for a single 
test result. It is possible that no control was used because QC was 
never performed.
(5) y = 1 to z. The value of z is the total number of reruns executed for a 
single test result.
53 Order ID
56 Sample Name 0 to 1
55 Test ID
1 to n
00 Result data
14 Validation status
06/08/09 Raw Data 1 to w
17 Test Execution
18 Cassette Information
19 Calibration Data
21 Control Data 0 to p
27 Rerun results 0 to y
y Block 04
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes for extended host interface 123
Block 05: Calibration Resultwith Lot Information
Block 05 is transmitted to the host if all of the following 
conditions are met:
• After receiving a result request with result type 
selector 11 or 18
• If an accepted and not yet transmitted calibration 
result is available
• If the instrument is configured accordingly
Line code Description Iteration(1)(2)(3)
(1) m = 1 to 6. m is the number of standard rates in line 03. The 
sequence of m iterations must correspond to the sequence of values 
in line 03. In addition, the number of iterations m has to be equal to 
the number of parameters (standard rates) in line 03.
(2) y = 0 or 1. The value of y depends on whether the antigen excess 
(AGE) check is switched ON or OFF. The instrument always transmits 
an AGE rate line regardless of this parameter. If the AGE check was 
not required by the test, this line contains the values initialized by the 
instrument.
(3) n = 1 to 423. The value of n can be different from 1 only if the result 
was measured using an absorbance test (Line 06). There are 423 
possible lines since each line is produced for every replicate (max. 3) 
and every cycle (max 141).
55 Test ID
15 Access Date Time
16 CALidx
03 Standard Rates 1 to y+1
04 Calibrate Curve
13 Calibrator Lot Information
11 Reagent Lot Information
00 Result Data
1 to m
06/08/09 Raw Data 1 to n
y Block 5
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
124 Result blocks
Block 06: Control Result with Lot Information
Block 06 is transmitted to the host if all of the following 
conditions are met:
• After receiving a result request with result type 
selector 11 or 19
• If an accepted and not yet transmitted control result is 
available
• If the instrument is configured accordingly
Results always collated by test ID Result type selector 11 or 19 is used.
• m = 1 (one test ID per result block)
• n = 1 to 6
q Results of different control orders are not collated.
Line code Description Iteration(1)
(1) w = 1 to 141. The value of w can be different from 1 only if the result 
was measured using an absorbance test (Line 06). There are 141 
possible lines since each line is produced for every cycle (max 141).
55 Test ID
1 to m
15 Access Date Time
16 CALidx
11 Reagent Lot 
Information
02 Control ID
1 to n
12 Control Lot 
Information
00 Result Data
06/08/09 Raw Data 1 to w
y Block 06
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes for extended host interface 125
Block 07: Sample Results with Lot Information
Block 07 is transmitted to the host if all of the following 
conditions are met:
• After receiving a result request with result type 
selector 11 or 17
• If an accepted and not yet transmitted sample result is 
available
Line code Description Iteration(1)(2)(3)
(1) w = 1 to 141. The value of w can be different from 1 only if the result 
was measured using an absorbance test (Line 06). There are 141 
possible lines since each line is produced for every cycle (max 141). 
(2) p = 0 to x. The value of x is the number of controls used for a single 
test result. It is possible that no control was used because QC was 
never performed. 
(3) y = 1 to z. The value of z is the total number of reruns executed for a 
single test result. 
53 Order ID
56 Sample Name 0 to 1
15 Access Date Time
16 CALidx
55 Test ID
11 Reagent Lot Information
00 Result data
14 Validation Status
06/08/09 Raw Data 1 to w
17 Test Execution
18 Cassette Information
19 Calibration Data
21 Control Data 0 to p
27 Rerun results 0 to y
y Block 07
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
126 Operation settings blocks
Operation settings blocks
In this section
Block 20: Operation Setting (126)
Block 21: Operation setting response (126)
Block 20: Operation Setting
The instrument can change the status after command 
from host. The commands can be Start and Stop.
The possible status changes are:
q If the instrument is set from Operating to Standby 
and tests are running, the tests are completed and then 
the instrument goes to Standby. 
Block 21: Operation setting response
Each request with Blocks 20 is answered by Block 21 and 
Line 96.
The response message depends on the success of the 
request.
Status before 
command
Status after 
command
Command
Sleeping Standby Start
Standby Operating Start
Operating Standby Stop
y Status change
Line code Description
30 Operation
y Block 20
Line code Description
96 Error Message
y Block 21
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes for extended host interface 127
Service Action Blocks
In this section
Block 25: Service Action Execution (127)
Block 26: Service Action response (127)
Block 27: Service Action Result Request (127)
Block 28: Service Action Result Response (128)
Block 29: Failed Service Action Result Response (128)
Block 25: Service Action Execution
Automatic Service Action can be performed from host. In 
order to perform the automatic service actions the system 
needs to be in Standby mode. The service action is 
specified by Line 32.
Block 26: Service Action response
Each request with Blocks 25 is answered by Block 26 and 
Line 96.
The response message depends on the success of the 
request.
Block 27: Service Action Result Request
It is possible to retrieve all service action execution results 
after the specified date and time.
Line code Description
32 Service Action
y Block 25
Line code Description
96 Error Message
y Block 26
Line code Description
24 Date and time
y Block 27
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
128 Service Action Blocks
Block 28: Service Action Result Response
This request provides all the service action execution 
results after date sent with Block 27.
Block 29: Failed Service Action Result Response
Block 29 contains Line 96 and provides the eventual error 
encountered following request with Block 27.
Line code Description Iteration(1)
(1) m is the total number of service actions executed after the specified 
date and time.
24 Date and time
1 to m
58 Service action information
y Block 28
Line code Description
96 Error Message
y Block 29
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes for extended host interface 129
Test status blocks
In this section
Block 45: Test status request (129)
Block 46: Test Status Response (129)
Block 47: Failed Test Status Request (129)
Block 45: Test status request
Block 45 is used to interrogate the test status. Block 45 
has no lines.
Block 46: Test Status Response
This response provides the test status for all loaded tests.
Block 47: Failed Test Status Request
Block 47 contains Line 96 and provides the eventual error 
encountered following request with Block 45.
Line code Description Iteration(1)(2)(3)(4)(5)
(1) m is the total number of loaded tests.
(2) x is the number of cassettes for a specific test. The maximum value 
is set to 6.
(3) y is the number of calibrators for a specific test. The maximum value 
is set to 6.
(4) z is the number of controls for a specifictest. The maximum value is 
set to 6.
(5) w is the number of diluents for a specific test. The maximum value is 
set to 6.
55 Test ID
1 to m
28 Test Status
29 Cassette Status 0 to x
31 Calibrator status 0 to y
33 Control status 0 to z
38 Diluents status 0 to w
y Block 46
Line code Description
96 Error Message
y Block 47
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
130 Calibration status blocks
Calibration status blocks
In this section
Block 52: Calibration status request (130)
Block 54: Calibration Status Response (130)
Block 57: Failed Calibration Status Request (130)
Block 52: Calibration status request
Block 52 is used to interrogate the calibration status. 
Block 52 has no lines.
Block 54: Calibration Status Response
This request provides the calibration status for all loaded 
tests.
Line 49 is added only if rack position information is 
available.
Block 57: Failed Calibration Status Request
Block 57 contains Line 96 and provides the eventual error 
encountered following request with Block 52.
Line code Description Iteration(1)
(1) m is the total number of loaded tests.
55 Test ID
1 to m
60 Calibration due
61 Test Status and 
blocked status
62 Calibrator ID 0 to 1
49 Rack Position 0 to 1
y Block 54
Line code Description
96 Error Message
y Block 57
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes for extended host interface 131
Cassette status blocks
In this section
Block 50: Cassette status request (131)
Block 55: Cassette Status Response (131)
Block 59: Failed Cassette Status Request (131)
Block 50: Cassette status request
Block 50 is used to interrogate the cassette status. Block 
50 has no lines.
Block 55: Cassette Status Response
This request provides the status for all cassettes on 
board.
 Block 59: Failed Cassette Status Request
Block 59 contains Line 96 and provides the eventual error 
encountered following request with Block 50.
Line code Description Iteration(1)
(1) m is the total number of cassettes on board. The maximum number 
is 32.
36 Cassette definition
1 to m
37 Tests left
39 Cassette Status
49 Rack Position
y Block 55
Line code Description
96 Error Message
y Block 59
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
132 Multi-configuration service block
Multi-configuration service block
In this section
Block 64: Service Action Status (132)
Block 64: Service Action Status
This request provides the status for all automatic service 
actions.
Line code Description Iteration(1)
(1) m is the total number of automatic service actions.
45 Service Action definition
1 to m
46 Current Time or tests 
elapsed
47 Service Due
48 Elapsed percentage
y Block 64
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes for extended host interface 133
Message Log blocks
In this section
Block 75: Message Log Request (133)
Block 76: Message Log Response (133)
Block 77: Failed Message Log Request (133)
Block 75: Message Log Request
It is possible to retrieve all the messages received by the 
control unit after the specified date and time.
Block 76: Message Log Response
This request provides all the messages in the message 
log received after date sent with block 75.
Block 77: Failed Message Log Request
Block 77 contains Line 96 and provides the eventual error 
encountered following request with Block 75.
Line code Description
24 Date and Time
y Block 75
Line code Description Iteration(1)(2)
(1) m is the total number of messages received after the specified date 
and time.
(2) n is the total length of the message string divided by 100 (rounded 
to the next integer).
24 Date and time
1 to m82 Message Icon
83 Message String 1 to n
y Block 76
Line code Description
96 Error Message
y Block 77
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
134 Sample order status blocks
Sample order status blocks
In this section
Block 80: Order Status Request (134)
Block 81: Order Status Response (134)
Block 85: Failed Order Status Request (135)
Block 80: Order Status Request
It is possible to retrieve the status of all pending sample 
orders or of a specific sample order.
If Line 20 is omitted all the pending sample orders are 
retrieved. 
If Line 20 is used the status of the specified sample will 
be retrieved regardless whether the sample is pending or 
not.
Block 81: Order Status Response
This response provides the sample order status for the 
requested items.
If the request was performed using block 80 with no line 
the total number of pending orders will be returned. 
Otherwise if line 20 was used in block 80 only the 
requestd sample will be returned (m=1). 
Line code Description Iteration
20 Order ID 0 to 1
y Block 80
Line code Description Iteration(1)(2)
(1) m is the total number of pending sample orders.
(2) n is the total number of blocked test for each sample order.
22 Order ID
1 to m23 Status
25 Test Status 0 to n
y Block 76
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes for extended host interface 135
Block 85: Failed Order Status Request
Block 85 contains Line 96 and provides the eventual error 
encountered following request with Block 80.
Line code Description
96 Error Message
y Block 85
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
136 System status blocks
System status blocks
In this section
Block 90: System status request (136)
Block 91: System status response (136)
Block 90: System status request
Block 90 is used to interrogate the system status. Block 
90 has no lines.
Block 91: System status response
Block 91 is the response to Block 90. It contains line 97.
Line code Description
97 System Status
y Block 91
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Block codes for extended host interface 137
Date and time blocks
In this section
Block 94: Date and Time request (137)
Block 95: Date and Time data (137)
Block 94: Date and Time request
Block 94 is used to interrogate the date and time of the 
system. Block 94 has no lines.
Block 95: Date and Time data
Block 95 is the answer to Block 94, Date and time 
request. Block 95 contains one line.
Line code Description
93 Date and Time
y Block 95
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
138 Serial number blocks
Serial number blocks
In this section
Block 96: Serial Number Request (138)
Block 97: Serial Number Response (138)
Block 96: Serial Number Request
Block 96 is used to interrogate the instrument serial 
number. Block 96 has no lines.
Block 97: Serial Number Response
Block 97is the answer to Block 96, Serial Number 
request. Block 97 contains one line.
Line code Description
94 Serial Number
y Block 97
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
 139
Table of contents
Line codes for extended host 
interface 8
Line codes form part of the data block of a message. They 
are used to identify the content of the line.
If the extended host interface of the 
Cedex Bio HT Analyzer is enabled, the following line 
codes are modified or can additionally be used.
In this chapter 8
About line codes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141
List of line codes for extended HIF. . . . . . . . . . . . . . . . 142
Line Code 06: Absorbance Raw Data . . . . . . . . . . 142
Line Code 08: FP Raw Data . . . . . . . . . . . . . . . . . . . 143
Line Code 09: ISE Raw Data . . . . . . . . . . . . . . . . . . 143
Line Code 14: Validation Status . . . . . . . . . . . . . . . 143
Line Code 17: Test Execution . . . . . . . . . . . . . . . . . 144
Line Code 18: Cassette Information . . . . . . . . . . . . 146
Line Code 19: Calibration Information . . . . . . . . . . 147
Line Code 20: Order ID . . . . . . . . . . . . . . . . . . . . . . 150
Line Code 21: Control Information . . . . . . . . . . . . . 150
Line Code 22: Order ID . . . . . . . . . . . . . . . . . . . . . . 153
Line Code 23: Sample Order Status . . . . . . . . . . . . 153
Line Code 24: Message Date Time . . . . . . . . . . . . 153
Line Code 25: Test Order Status . . . . . . . . . . . . . . . 154
Line Code 26: Dilution Factor . . . . . . . . . . . . . . . . . 155
Line Code 27: Rerun Result. . . . . . . . . . . . . . . . . . . 156
Line Code 28: Test Status . . . . . . . . . . . . . . . . . . . . 156
Line Code 29: Cassette status. . . . . . . . . . . . . . . . . 157
Line Code 30: Operation selection . . . . . . . . . . . . . 157
Line Code 31: Calibrator status. . . . . . . . . . . . . . . . 157
Line Code 32: Service Action selection . . . . . . . . . 158
Line Code 33: Control status. . . . . . . . . . . . . . . . . . 158
Line Code 36: Cassette definition. . . . . . . . . . . . . . 158
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
140
Table of contents
Line Code 37: Tests left . . . . . . . . . . . . . . . . . . . . . . 159
Line Code 38: Diluent status . . . . . . . . . . . . . . . . . . 159
Line Code 39: Cassette Status . . . . . . . . . . . . . . . . 160
Line code 40: Service Selection . . . . . . . . . . . . . . . 161
Line Code 45: Service Action Definition . . . . . . . . 161
Line Code 46: Currently Time or Tests elapsed . . 162
Line Code 47: Service Due . . . . . . . . . . . . . . . . . . . 162
Line Code 48: Elapsed percentage. . . . . . . . . . . . . 162
Line Code 49: Rack Position . . . . . . . . . . . . . . . . . . 163
Line code 55: Test ID . . . . . . . . . . . . . . . . . . . . . . . . 163
Line Code 58: Service action result . . . . . . . . . . . . 164
Line Code 60: Calibration due. . . . . . . . . . . . . . . . . 165
Line Code 61: Test Status and blocked status . . . 166
Line Code 62: Calibrator ID . . . . . . . . . . . . . . . . . . . 167
Line Code 82: Message Icon. . . . . . . . . . . . . . . . . . 167
Line Code 83: Message String . . . . . . . . . . . . . . . . 169
Line Code 93: System Date Time . . . . . . . . . . . . . . 169
Line Code 94: Serial Number . . . . . . . . . . . . . . . . . 169
Line Code 97: System status . . . . . . . . . . . . . . . . . . 170
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes for extended host interface 141
About line codes
Format code Description
Sx x-character ASCII-string, left adjusted.
Length x is fixed.
Shorter strings have to be filled up with trailing <spaces>.
Example: S15 "Mr.•Evans••••••"
Ix ASCII-formatted x-digit integer value, right adjusted.
Length x is fixed.
Shorter integer values have to be filled up with leading <spaces> or zeros (0).
Example: I5 "00069" or "•••69"
SRx.y ASCII-formatted real value with sign, right adjusted.
x = Length of the whole data record.
y = Number of digits after the decimal point (optional).
Length x is fixed.
Shorter real-values have to be filled up with leading <spaces> or zeros (0).
The decimal point is mandatory.
Example: SR9.3: "-0077.123"
Fx ASCII formatted floating point number, right adjusted.
x = Length of the whole data record.
Length x is fixed.
Shorter real-values have to be filled up with leading <spaces> or zeros (0).
The sign "+" or "-" for the exponent is mandatory.
Decimal point and exponent "E" are mandatory.
Mantissa has exactly one digit before the decimal point in the range 1 to 9.
No <spaces> are allowed between the mantissa and the exponent.
Example: F12: "••••6.11E+33" or F15: "-00007.4321E-02"
D1 Date format 1: DD/MM/YYYY.
10 character ASCII-string.
Example: "04/01/1994"
D2 Date format 2: DD-MMM-YY (used by COBAS Core/Core II).
9 character ASCII-string.
Example: "04-JAN-94"
T1 Time format 1: hh:mm:ss (hh in 24 h format).
8 character ASCII-string.
Example: "22:07:34"
T2 Time format 2: hh:mm (hh in 24 h format) (used by COBAS Core/Core II).
5 character ASCII-string.
Example: "22:07"
y Formats of data fields used in line codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
142 List of line codes for extended HIF
List of line codes for extended HIF
Line Code 06: Absorbance Raw Data 
Example 06_•49_1_•0.0363_•0.0046<LF>
n is the total number of wavelength used and can go up 
to 12. Hence there can be up to 12 floating point numbers 
in the same Line.
Line code In Line code In
06 Absorbance Raw Data 36 Cassette definition
08 FP Raw Data 37 Tests left
09 ISE Raw Data 38 Diluent status
14 Validation Status 39 Cassette Status
17 Test Execution 40 Service Selection
18 Cassette Information 45 Service Action Definition
19 Calibration Information 46 Currently Time or Tests elapsed
20 Order ID (Request) 47 Service Due
21 Control Information 48 Elapsed percentage
22 Order ID (Response) 49 Rack Position
23 Sample Order Status 55 Test ID
24 Message Date Time 58 Service action result
25 Test Order Status 60 Calibration due
26 Dilution Factor 61 Test Status and blocked status
27 Rerun Result 62 Calibrator ID
28 Test Status 82 Message Icon
29 Cassette status 83 Message String
30 Operation selection 93 System Date Time
31 Calibrator status 94 Serial Number
32 Service Action selection 97 System status
33 Control status
y List of line codes
Description Format Range
Line Code 06
Cycle I3 1 to 141
Replicate Number I1 1 to 3
Absorbance Data F7 1 to n
y Line Code 06: Absorbance Raw Data 
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes for extended host interface 143
Line Code 08: FP Raw Data
Control 08_114190150_114190152_•••••••••0<LF> 
q Notes regarding the results replicates:
The 4 fields will be replicated r times in the same Line.
r = Total number of replicates
Line Code 09: ISE Raw Data 
Example 09_••78.20<LF>
Line Code 14: Validation Status
Example 14_1<LF>
Description Format Range
Line Code 08
Blank parallel I9 1 to r
Blank vertical I9
Test parallel I9
Test vertical I9
y Line Code 08: FP Raw Data
Description Format Range$
Line Code 09
ISE Mean Value F7
y Line Code 09: ISE Raw Data 
Description FormatRange Range description
Line Code 14
Validation Status I1 0 Accepted
1 Disabled
2 Rerun
3 Not handled
y Line Code 14: Validation Status
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
144 List of line codes for extended HIF
Line Code 17: Test Execution
Example 17_23/04/2006_22:07:59_1:1•••_••0<LF>
q oExecution Date and Time is the point when the 
measurements are executed on the Instrument (Calculation 
Date Time)
o If the dilution flag is shorter than six characters, the 
string will be filled with spaces on the right side.
Example, 1:1 will be 1:1••• 
(•) represents a filler space 
o If the dilution flag is shorter than three characters, the 
string will be filled with spaces on the left side.
Example, 0 will be••0 
(•) represents a filler space 
Description Format Range Range description
Line Code 17
Date D1 dd/mm/yyyy
Time T1 hh:mm:ss
Dilution Flag S6 1:X with X going from 
1 to 100
Sample Flag I3 Refer to following 
table
y Line Code 17: Test Execution
Flag code Description
13 Result calculated using an erroneous or corrected calibration which has been accepted. (F1)
69 Result calculated using a postponed calibration which has been accepted. Note: Postponing a 
calibration may lead to erroneous results. Action: Review results - Repeat calibration if necessary.
32 Result is associated with an erroneous or corrected control. The control has been accepted. (F1).
34 Result (or a replicate) is erroneous or corrected. Result has been accepted. Recommended action: 
Check results reaction curve to establish the cause of the deviation; repeat sample measurement (F1)
10 (Serum proteins only) The sample contains an excess of antigen. %1% = [value] - is Test Range High 
(F3)
12 Insufficient calibrator or too many air bubbles. The calibrator segment is too short or air bubbles are 
present, or a tube is leaking or blocked. (F3)
11 Insufficient amount of sample/QC/Solution or too many air bubbles in electrode path. The sample 
segment is too short or air bubbles are present, mixing is too vigorous (in the case of the indirect/urine 
mode) or a tube is leaking or blocked. (F3)
255 Any test flag.
15 Calculation error from pre-calculation (Abs / Fp raw data)
65 Result obtained using a concentration factor. (F1)
17 The dark measurement was higher than the test measurement. (F3)
62 Result differs from last result (up to n days before) by greater than defined %. %1% = [def. value] in 
percent (F4)
61 Result differs from last result (up to n days before) by greater than defined value. %1% = [def. value] 
%2% = [unit] (F4)
64 Result obtained using a dilution factor. (F1)
y Flag codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes for extended host interface 145
18 The values near the endpoint fluctuate. (F3)
19 The result from the parallel or perpendicular measurement was inconsistent. Air bubbles in the sample 
may be the cause. (F3)
68 Hemolysis sample present The sample turn to red. (F2)
20 The measured absorbance is higher than 2.0. (F3)
21 High Activity. The absorbance change during measurement exceeds the accepted limit, i.e . the reaction 
has taken off too fast. Possibility of substrate exhaustion. %1% = [value] - is Test Range High (F3)
22 High Background.Background fluorescence (noise) from the sample may interfere with FP 
measurement. (F3)
52 The value exceeds the critical high value defined for the test. (F4)
58 TDM result only.Value is above peak region. %1% = [value] %2% = [unit] (F4)
54 The value is above the reference range. %1% = [value] (F4)
9 The result exceeds than the upper measurable test range. (F3)
60 TDM result only.Value is above trough region. %1% = [value] %2% = [unit] (F4)
67 Icteria sample present The sample turn to yellow. (F2)
23 ISE Measurement Unstable. The mV values from the electrode are not stable. The following problems 
may have occurred: - Air bubbles in measuring segment. - One or more sealing rings is missing or 
damaged. - Electrode has not been serviced. - Expiry period of the electrode has been exceeded or the 
electrode is damaged. - Preamplifier is not working properly. (F3)
51 The value falls below the critical low value defined for the test. (F4)
1 Not enough readings in kinetic range for calc mode Kinetic or Kinsearch. (F3)
57 TDM result only.Value is below peak region. %1% = [value] %2% = [unit] (F4)
59 TDM result only.Value is below trough region. %1% = [value] %2% = [unit] (F4)
2 Low Activity. The absorbance change during measurement doesn't reached the accepted limit, i.e . the 
reaction has taken off too slow.
24 The intensity obtained in the parallel or perpendicular measurement is insufficient. (F3)
53 The value falls below the reference range. %1% = [value] (F4)
4 The result is lower than the measurable test range. (F3)
26 The sensor could not detect a calibrator. ISE Calibrator bottles are not available or the tubing is blocked 
or leaking. (F3)
25 The sensor could not detect any liquid. Either the liquid has not been pipetted, the pipetted sample was 
lipemic, or the ISE tubing was blocked. (F3)
27 Unable to calculate the kinetic change. Either change is not stable (Kinsearch) or "non-existent" 
(Kinetic). The first and last part of the reaction have very different slopes. (F3)
0 No Flag.
30 Too many outliers occurred in the parallel or perpendicular FP measurement. Air bubbles in the sample 
or electronic noise may be the cause. Recommended action: Repeat the measurement with a sample 
free of bubbles; Inform Roche Service is flag appears again. (F3)
31 The parallel measurement was higher than the perpendicular measurement. Recommended action: 
Repeat the measurement. Inform Roche Service if flag appears again. (F3)
55 The result is higher than or equal to the cutoff value. This flag appears with quantitative tests and is used 
mainly for drugs of abuse tests. %1% = [value] %2% = [unit] (F4)
56 The result is higher than or equal to the cutoff value. This flag appears with qualitative tests and is used 
mainly for drugs of abuse tests. (F4)
63 Result obtained after a repeated run. (F1)
37 No end of calibrator segment detected. It takes too long for a calibrator segment to reach the electrode 
assembly. This may be due to the lack of calibrator or the tubing may be blocked or leaking. (F3)
36 No end of sample segment detected. It takes too long for a sample segment to reach the electrode 
assembly. The tubing may be blocked or leaking. (F3)
Flag code Description
y Flag codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
146 List of line codes for extended HIF
Line Code 18: Cassette Information
Example 18_62159401••_68354•••••_12/05/2016_06/11/2017<LF>
q oIf no cassette lot number or serial number are found or 
they were not defined, "??????????" will be used and 
transmitted (without quotes).
o If no on board expiration date or cassette lot 
expiration date are given for the cassette, 
"00/00/0000" will be used and transmitted (without 
quotes).
o If the lot number or serial number are shorter than ten 
characters, the string will be filled with spaces on the 
right side. 
Example, 62159401 will be 62159401••
(•) represents a filler space 
66 turbitidy sample present The sample has a cloudy color. (F2)
14 Result calculated using an erroneous or corrected calibration which has not been accepted. (F1)
70 Result calculated using a postponed calibration which has not been accepted. Note: Postponing a 
calibration may lead to erroneous results Action: Check carefully the results- Repeat calibration if 
necessary.
33 Result is associated with an erroneous or corrected control. The control has not been accepted. 
Recommended action: repeat measurement with a fresh control; repeat sample measurement if 
necessary. (F1).
35 Result (or a replicate) is erroneous or corrected. Result has not been accepted. Recommended action: 
Check results reaction curve to establish the cause of the deviation; repeat sample measurement. (F1)
Flag code Description
y Flag codes
Description Format Range
Line Code 18
Cassette lot number S10
Cassette serial number S10
Cassette on board expiration 
date
D1 dd/mm/yyyy
Cassette lot expiration date D1 dd/mm/yyyy
y Line Code 18: Cassette Information
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes for extended host interface 147
Line Code 19: Calibration Information 
Example 19_11/04/2016_09:00:12_07-3718-
6•_176121••••_30/05/2016_62159401••_68354•••••_???
???????_00/00/0000_??????????_00/00/0000_?????????
?_00/00/0000_R0••••••_0.0007479•_F•••••••••_5286.7
620•_??????????_??????????_??????????_??????????_?
?????????_??????????_••0<LF>
Description Format Range
Line Code 19
Calibration Date D1 dd/mm/yyyy
Calibration Time T1 hh:mm:ss
Calibrator ID S10
Calibrator Lot Number S10
Calibrator Lot Expiration Date D1 dd/mm/yyyy
Cassette Lot Number used for Calibration S10
Cassette Serial Number used for Calibration S10
Calibrator ISE Solution 1 Lot Number S10
Calibrator ISE Solution 1 Expiration Date D1 dd/mm/yyyy
Calibrator ISE Solution 2 Lot Number S10
Calibrator ISE Solution 2 Expiration Date D1 dd/mm/yyyy
Calibrator ISE Solution 3 Lot Number S10
Calibrator ISE Solution 3 Expiration Date D1 dd/mm/yyyy
Calibration Result Coefficient 1 Label S10
Calibration Result Coefficient 1 S10
Calibration Result Coefficient 2 Label S10
Calibration Result Coefficient 2 S10
Calibration Result Coefficient 3 Label S10
Calibration Result Coefficient 3 S10
Calibration Result Coefficient 4 Label S10
Calibration Result Coefficient 4 S10
Calibration Result Coefficient 5 Label S10
Calibration Result Coefficient 5 S10
Calibrator Flag I3 Detailed information in the table below
y Line Code 19: Calibration Information 
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
148 List of line codes for extended HIF
q oIf no calibrator lot number was defined, "??????????" will 
be used and transmitted (without quotes).
o If no calibrator lot expiration date is given, 
"00/00/0000" will be used and transmitted (without 
quotes). 
o If no cassette lot number and serial number used for 
calibration was defined, "??????????" will be used and 
transmitted (without quotes).
o If no ISE Solution 1-3 lot number are given, 
"??????????" will be used and transmitted (without 
quotes).
o If no ISE Solution 1-3 lot expiration date are given, 
"00/00/0000" will be used and transmitted (without 
quotes).
o If no Calibration Result Coefficients 1-5 are given, 
"??????????" will be used and transmitted (without 
quotes).
o If the any lot number or coefficient labels/values are 
shorter than ten characters, the string will be filled 
with spaces on the right side. 
Example, 68354 will be 68354 •••••• 
(•) represents a filler space 
o If the calibrator flag is shorter than three characters, 
the string will be filled with spaces on the left side.
Example, 0 will be••0 
(•) represents a filler space 
Flag code Description
0 No Flag
1 Not enough readings in kinetic range for calc mode Kinetic or Kinsearch. (F3)
2 Low Activity. The absorbance change during measurement doesn't reached the accepted limit, i.e . the 
reaction has taken off too slow.
3 Absorbance values of the lowest concentration calibrator fall below the lower range. For general 
chemistries this is often the reagent blank. (F3)
4 The result is lower than the measurable test range. (F3)
5 Curve Direction is not corresponding with the testdefinition curve direction. (F3)
6 Absorbance values of the lowest concentration calibrator fall is above the upper range. (F3)
7 Deviation between replicates exceed programmed limit. One or more replicates are erroneous. (F3)
8 Recovered values of standards deviate greater than programmed limit. (F3)
9 The result exceeds than the upper measurable test range. (F3)
10 (Serum proteins only) The sample contains an excess of antigen. %1% = [value] - is Test Range High 
(F3)
11 Insufficient amount of sample/QC/Solution or too many air bubbles in electrode path. The sample 
segment is too short or air bubbles are present, mixing is too vigorous (in the case of the indirect/urine 
mode) or a tube is leaking or blocked. (F3)
12 Insufficient calibrator or too many air bubbles. The calibrator segment is too short or air bubbles are 
present, or a tube is leaking or blocked. (F3)
15 Nonlinear standard curve cannot be calculated due to calculation error. Action: Check calibration 
graph.Rerun calibration with fresh calibrators. (F3)
y Flag codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
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16 Result (or replicate) has been deleted. (F1)
17 The dark measurement was higher than the test measurement. (F3)
18 The values near the endpoint fluctuate. (F3)
19 The result from the parallel or perpendicular measurement was inconsistent. Air bubbles in the sample 
may be the cause. (F3)
20 The measured absorbance is higher than 2.0. (F3)
21 High Activity. The absorbance change during measurement exceeds the accepted limit, i.e . the reaction 
has taken off too fast. Possibility of substrate exhaustion. %1% = [value] - is Test Range High (F3)
22 High Background.Background fluorescence (noise) from the sample may interfere with FP 
measurement. (F3)
23 ISE Measurement Unstable. The mV values from the electrode are not stable. The following problems 
may have occurred: - Air bubbles in measuring segment. - One or more sealing rings is missing or 
damaged. - Electrode has not been serviced. - Expiry period of the electrode has been exceeded or the 
electrode is damaged. - Preamplifier is not working properly. (F3)
24 The intensity obtained in the parallel or perpendicular measurement is insufficient. (F3)
25 The sensor could not detect any liquid. Either the liquid has not been pipetted, the pipetted sample was 
lipemic, or the ISE tubing was blocked. (F3)
26 The sensor could not detect a calibrator. ISE Calibrator bottles are not available or the tubing is blocked 
or leaking. (F3)
27 Unable to calculate the kinetic change. Either change is not stable (Kinsearch) or "non-existent" 
(Kinetic). The first and last part of the reaction have very different slopes. (F3)
28 Curve is not monotonic (constantly decreasing or increasing). Curve cannot be calculated. (F3)
29 Calibration curve or slope does not fall within programmed range. (F3)
30 Too many outliers occurred in the parallel or perpendicular FP measurement. Air bubbles in the sample 
or electronic noise may be the cause. Recommended action: Repeat the measurement with a sample 
free of bubbles; Inform Roche Service is flag appears again. (F3)
31 The parallel measurement was higher than the perpendicular measurement. Recommended action: 
Repeat the measurement. Inform Roche Service if flag appears again. (F3)
34 Result (or a replicate) is erroneous or corrected. Result has been accepted.Recommended action: Check 
results reaction curve to establish the cause of the deviation; repeat sample measurement. (F1)
35 Result (or a replicate) is erroneous or corrected.Result has not been accepted.Recommended action: 
Check results reaction curve to establish the cause of the deviation; repeat sample measurement. (F1)
36 No end of sample segment detected. It takes too long for a sample segment to reach the electrode 
assembly. The tubing may be blocked or leaking. (F3)
37 No end of calibrator segment detected. It takes too long for a calibrator segment to reach the electrode 
assembly. This may be due to the lack of calibrator or the tubing may be blocked or leaking. (F3)
38 The solution 1 factor is outside the acceptable range. (F3)
63 Result obtained after a repeated run. (F1)
71 New calibration overwritten with old accepted calibration
255 Any Calibration-Flag.
Flag code Description
y Flag codes
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Line Code 20: Order ID 
Example 20_abcdef987654321<LF>
q The order number and the sample type may not 
contain <space>s in front of or within the identifier. 
Nevertheless, if the fields are not used in their full length, 
digits left over have to be filled with <space>s.
Line Code 21: Control Information
Example 21_22/04/2016_08:31:37_07-7470-
7•_186387••••_31/05/2018_62159401••_68354•••••9.99
9999E+99_U/L•••_•17<LF>
Description Format Range
Line Code 20
Order number S15 Sample Order ID
y Line Code 20: Order ID 
Description Format Range
Line Code 21
Control Date D1 dd/mm/yyyy
Control Time T1 hh:mm:ss
Control ID S10
Control Lot Number S10
Control Lot Expiration Date D1 dd/mm/yyyy
Cassette Lot Number used 
for Control
S10
Cassette Serial Number 
used for Control
S10
Control Result F13
Control Unit S6
Control Flag I3 Refer to following 
table
y Line Code 21: Control Information
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
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q oIf Cassette Lot Number or Serial Number are used for 
Control are defined, "??????????" will be used and 
transmitted (without quotes).
o If the Control ID, Control Lot Number, Cassette Lot 
Number or Serial Number used for Control are shorter 
than ten characters, they will be filled with spaces on 
the right side. 
Example, 68354 will be 68354••••• 
(•) represents a filler space
o If the Control Unit string is shorter than six characters, 
it will be filled with spaces on the right side. 
Example, U/L will be U/L••• 
(•) represents a filler space 
o If the control flag is shorter than three characters, the 
string will be filled with spaces on the left side. 
Example, 17 will be •17 
(•) represents a filler space 
Flag code Description
13 Result calculated using an erroneous or corrected calibration which has been accepted. (F1)
69 Result calculated using a postponed calibration which has been accepted. Note: Postponing a 
calibration may lead to erroneous results Action: Review results - Repeat calibration if necessary.
34 Result (or a replicate) is erroneous or corrected. Result has been accepted. Recommended action: 
Check results reaction curve to establish the cause of the deviation; repeat sample measurement. (F1)
10 (Serum proteins only) The sample contains an excess of antigen. %1% = [value] - is Test Range High 
(F3)
12 Insufficient calibrator or too many air bubbles. The calibrator segment is too short or air bubbles are 
present, or a tube is leaking or blocked. (F3)
11 Insufficient amount of sample/QC/Solution or too many air bubbles in electrode path. The sample 
segment is too short or air bubbles are present, mixing is too vigorous (in the case of the indirect/urine 
mode) or a tube is leaking or blocked. (F3)
255 Any Control-Flag.
15 Calculation error from pre-calculation (Abs / Fp raw data)
65 Result obtained using a concentration factor. (F1)
17 The dark measurement was higher than the test measurement. (F3)
64 Result obtained using a dilution factor. (F1)
18 The values near the endpoint fluctuate. (F3)
19 The result from the parallel or perpendicular measurement was inconsistent. Air bubbles in the sample 
may be the cause. (F3)
20 The measured absorbance is higher than 2.0. (F3)
21 High Activity. The absorbance change during measurement exceeds the accepted limit, i.e . the reaction 
has taken off too fast. Possibility of substrate exhaustion. %1% = [value] - is Test Range High (F3)
22 High Background.Background fluorescence (noise) from the sample may interfere with FP 
measurement. (F3)
48 The accuracy of the result is outside the permitted range of an accuracy control (F4)
9 The result exceeds than the upper measurable test range. (F3)
49 The value exceeds the upper limit of a limit control. (F4)
y Flag codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
152 List of line codes for extended HIF
23 ISE Measurement Unstable. The mV values from the electrode are not stable. The following problems 
may have occurred: - Air bubbles in measuring segment. - One or more sealing rings is missing or 
damaged. - Electrode has not been serviced. - Expiry period of the electrode has been exceeded or the 
electrode is damaged. - Preamplifier is not working properly. (F3)
1 Not enough readings in kinetic range for calc mode Kinetic or Kinsearch. (F3)
50 The value is below the lower limit of a limit control. (F4)
2 Low Activity. The absorbance change during measurement doesn't reached the accepted limit, i.e . the 
reaction has taken off too slow.
24 The intensity obtained in the parallel or perpendicular measurement is insufficient. (F3)
4 The result is lower than the measurable test range. (F3)
26 The sensor could not detect a calibrator. ISE Calibrator bottles are not available or the tubing is blocked 
or leaking. (F3)
25 The sensor could not detect any liquid. Either the liquid has not been pipetted, the pipetted sample was 
lipemic, or the ISE tubing was blocked. (F3)
27 Unable to calculate the kinetic change. Either change is not stable (Kinsearch) or "non-existent" 
(Kinetic). The first and last part of the reaction have very different slopes. (F3)
0 No Flag.
30 Too many outliers occurred in the parallel or perpendicular FP measurement. Air bubbles in the sample 
or electronic noise may be the cause. Recommended action: Repeat the measurement with a sample 
free of bubbles; Inform Roche Service is flag appears again. (F3)
31 The parallel measurement was higher than the perpendicular measurement. Recommended action: 
Repeat the measurement. Inform Roche Service if flag appears again. (F3)
44 Westgard rule 5: Difference between two consecutive control values is greater than 4 SD. Action: rerun 
with fresh control; if flag repeats, check calibration, reagent (age) and the course of the reaction. (F4)
47 Westgard rule 1: one control value deviates from the mean (in either direction) by more than 2 SD but 
less than 3 SD. (F4)
46 Westgard rule 2: one control value deviates from the mean (in either direction) by more than 3 SD. (F4)
39 Westgard rule 6: Ten consecutive values appear on the same side of the mean. (F4)
45 Westgard rule 3: Two consecutive control values deviate from the mean (in the same direction) by more 
than 2 SD but less than 3 SD. (F4)
43 Westgard rule 4: Four consecutive control values deviate from the mean in the same direction by more 
than 1 SD. (F4)
42 Precision rule: Seven consecutive values decreased. (F4)
41 Precision rule: Seven consecutive values increased. (F4)
40 Precision rule: Seven consecutive values appear on the same side of the mean. (F4)
63 Result obtained after a repeated run. (F1)
37 No end ofcalibrator segment detected. It takes too long for a calibrator segment to reach the electrode 
assembly. This may be due to the lack of calibrator or the tubing may be blocked or leaking. (F3)
36 No end of sample segment detected. It takes too long for a sample segment to reach the electrode 
assembly. The tubing may be blocked or leaking. (F3)
14 Result calculated using an erroneous or corrected calibration which has not been accepted. (F1)
70 Result calculated using a postponed calibration which has not been accepted. Note: Postponing a 
calibration may lead to erroneous results Action: Check carefully the results - Repeat calibration if 
necessary.
35 Result (or a replicate) is erroneous or corrected. Result has not been accepted. Recommended action: 
Check results reaction curve to establish the cause of the deviation; repeat sample measurement. (F1)
Flag code Description
y Flag codes
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Line Code 22: Order ID
Example 22_abcdef987654321<LF>
Line Code 23: Sample Order Status
Example 23_3<LF>
Line Code 24: Message Date Time
Example 24_23/04/2006_22:07:59<LF>
q The messages are not cleared via host interface so, 
after a certain time, they should be deleted at the control 
unit.
Description Format Range
Line Code 22
Order number S15 Sample Order ID
Optional If sample name information is present
Sample Name S50
y Line Code 22: Order ID
Description Format Range Range description
Line Code 23
Status I2 3 blocked
4 not on board
7 no orders
10 calculated
19 new
30 running
y Line Code 23: Sample Order Status
Description Format Range
Line Code 24
Date D1 dd/mm/yyyy
Time T1 hh:mm:ss
y Line Code 24: Message Date Time
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Line Code 25: Test Order Status
Example 25_131_6_24<LF>
Description Format Range
Line Code 25
Test ID I3 1 to 999
Test Status I1 Refer to following 
table for details
Test Blocked Status I2 Refer to following 
table for details
y Line Code 25: Test Order Status
Status flag code Description
0 New
1 To be rerunned
2 Calculated
3 Running
4 Finished
5 Failed
6 Blocked
y Status flag codes
Blocking status 
flag code
Description
0 Okay
1 No fluid in sample cup
2 Reagent not on board
3 Reagent is empty
4 No cassette definition found
5 Diluent not on board
6 Diluent is empty
7 No diluent definition found
8 No calibrator on board
9 Calibrator empty
10 No valid calibration data
11 No calibration defintion available
12 No lot values for calibration available
13 Last calibration is not accepted
14 Control not on board
15 Control empty
16 Control is not valid
17 No control definition available
18 No assigned values for control available
19 Last control not accepted
20 Repeat action not possible
21 Test disabled
y Blocking status flag codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
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Line Code 26: Dilution Factor 
Example 26_••••2.1<LF>
q Samples on the instrument can only be diluted. The 
maximum dilution factor is 100.
22 Error in cuvette handling
23 Test aborted
24 Calculation not possible
25 No ISE Electrode
26 Activation not performed
27 ISE module not ready
28 Error in ISE module
29 Error in Abs. module
30 Error in Fp Module
31 Fatal HW Error
32 Activator not on board
33 Activator is empty
34 No activator definition found
35 Etcher not on board
36 Etcher is empty
37 No etcher definition found
38 Deproteinizer not on board
39 Deproteinizer is empty
40 No deproteinizer definition found
41 ISE module disabled
42 Fp module disabled
43 Cleaner not on board
44 Cleaner empty
45 No cleaner definition available
46 No ISE solution avaiable
47 No ISE solution definition avaiable
48 ISE solution is empty
49 Clot detected in sample
50 Cassette must be mixed first
Blocking status 
flag code
Description
y Blocking status flag codes
Description Format Range
Line Code 26 .
Dilution Factor F7 1 to 100
y Line Code 26: Dilution Factor 
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
156 List of line codes for extended HIF
Line Code 27: Rerun Result
Example 27_•8.694475E+01_•2_1:1•••<LF>
q If the result could not be calculated, the 
concentration value is returned as 9.99E+99.
Line Code 28: Test Status
Example 28_17_•0_•••0_N_2_<LF>
Description Format Range Range description
Line Code 27
Sample Concentration F13 +/-9.999999E+99 to +/-1.0E-99
Rerun Order I2
Dilution Flag S6 1:X with X going from 1 to 100
y Line Code 27: Rerun Result
Description Format Range Range description
Line Code 28
Test status I2 3 Blocked
16 Disabled
17 OK
31 Calibration postponed
Blocked status I2 For detailed information, refer to table in line 61.
Control Interval I4 Number of tests or hours between required control. 
Refer to next field for unit.
Control Type S1 N none (only if no control interval is set)
H hours
T tests
Calibration due status I1 1 None
2 Next Cassette
3 Next Lot
4 Required
Optional If the calibration is required
Calibration date D1 When is the next calibration due.
y Line Code 28: Test Status
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Line Code 29: Cassette status
Example 29_07-6831-6••••••_•1_G•7-1<LF>
Line Code 30: Operation selection
Example 30_1<LF>
Line Code 31: Calibrator status
Example 31_07-6830-8••••••_CFAS••••_•6_•1_J•13-6••••<LF>
Description Format Range
Line Code 29
Cassette ID S15 Number ID of the cassette
Cassette Status I2 For detailed information, refer to table displayed in 
Line 39.
Optional If the rack position is available
Rack Position S10 SlotID_RackNumber-RackPosition
y Line Code 29: Cassette status
Description Format Range Range description
Line Code 30
Operation setting I1 1 Start the 
instrument
2 Stop the 
instrument
y Line Code 30: Operation selection
Description Format Range Range description
Line Code 31
Calibrator ID S15 Number ID of the calibrator
Calibrator Short Name S8 Short name of the calibrator
Std I2 Standard number of the calibrator lot
Calibrator status I2 0 OK
1 Expired
2 Empty
3 Blocked
4 Not on board
22 Calibration Pending
Optional If the rack position is available
Rack Position S10 SlotID_RackNumber-RackPosition
y Line Code 31: Calibrator status
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
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Line Code 32: Service Action selection
Example 32_•1<LF>
Line Code 33: Control status
Example 33_97-0000-0••••••_•1_I•11-14•••<LF>
Line Code 36: Cassette definition
Description Format Range Range description
Line Code 32
Service Action I2 1 Deproteinize probes
2 Prime Fluids
3 Backup
4 Initialize ISE module
5 Electrode Service
6 Activate Electrodes
7 Prime ISE Calibrators
8 Clean ISE Tower
9 Condition ISE Tubing
y Line Code 32: Service Action selection
Description Format Range Range description
Line Code 33
Control IDS15 Number ID of the control
Control status I2 0 OK
1 Expired
2 Empty
3 Blocked
4 Not on board
32 Clot detected
Optional If the rack position is available
Rack Position S10 SlotID_RackNumber-RackPosition
y Line Code 33: Control status
Description Format Range
Line Code 36
Serial number S10 Cassette serial number
Lot number S10 Cassette Lot number
Cassette definition ID S10 ID of the cassette type
y Line Code 36: Cassette definition
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes for extended host interface 159
Example 36_39901•••••_12398201••_07-6494-9•<LF>
Line Code 37: Tests left
Example 37_•••0_•••0_•85.5<LF>
q If the cassette is a cleaner the Tests left and Total 
tests fields will be set to 0.
Line Code 38: Diluent status
Example 38_07-5635-0••••••_•1_O•140-8•••<LF>
Description Format Range
Line Code 37
Tests left I4 The number of tests left for the specific cassette
Total tests I4 The total number of tests originally available for the 
specific cassette
Optional If the cassette is a cleaner
Percentage of filling F5 The filling percentage when the cassette is a 
cleaner.
y Line Code 37: Tests left
Description Format Range Range description
Line Code 38
Diluent ID S15 Number ID of the diluent
Diluent status I2 0 OK
1 Expired
2 Empty
4 Not on board
6 Missing information
8 Low
9 Near expiry
12 In use
19 New
20 Ready
33 Preparing
Optional If the rack position is available
Rack Position S10 SlotID_RackNumber-RackPosition
y Line Code 38: Diluent status
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
160 List of line codes for extended HIF
Line Code 39: Cassette Status
Example 39_19_19/02/2016_13/05/2016<LF>
q Date opened and date expired will be rendered as 
00/00/0000 if the information is not available.
Every status can be mapped to one of three colours (Red, 
Yellow and Green). The following table shows the 
possible returned status with corresponding meaning and 
colour.
Description Format Range
Line Code 39
Status I2 Detailed information is 
provided in the table below
Date opened D1 dd/mm/yyyy
Date expired D1 dd/mm/yyyy
y Line Code 39: Cassette Status
Status code Meaning Color
12 In use Green
19 New cassette Green
20 Ready Green
1 Expired Yellow
5 Not enough for worklist Yellow
8 Low Yellow
9 Near expiration Yellow
13 Calibration expired Yellow
14 Requires mixing Yellow
15 Reconstituting Yellow
33 Preparing Yellow
2 Empty Red
4 Not on board Red
6 Missing definition (unknown 
fluid)
Red
y Status codes
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Line code 40: Service Selection
Example 40_1<LF>
Line Code 45: Service Action Definition 
Example 45_103_c<LF>
Description Format Range Range description
Line code 40
Service request I1 0 Slot configuration
1 Sample tubes without 
orders
3 Reserved
4 List of all sample tubes
5 Status of all automatic 
service actions
y Line code 40: Service Selection
Description Format Range Range description
Line Code 45
Service Action ID I3
Counts unit S1 h counts are given in 
hours 
c counts are given in 
test counts
y Line Code 45: Service Action Definition 
ID Description
0 Deproteinize probes
101 Prime fluid system
103 Backup database
301 Electrode service
304 Activate electrodes
310 Prime ISE calibrators
312 Clean ISE tower automatically
314 Condition ISE tubing
315 Initialize ISE module
y IDs
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162 List of line codes for extended HIF
Line Code 46: Currently Time or Tests elapsed 
Example 46_••••••1230_•••••••••3<LF>
Line Code 47: Service Due
Example 47_••••••2000_•••••••••5<LF>
Line Code 48: Elapsed percentage
Example 48_••••••••40_•••••••••32<LF>
Description Format Range
Line Code 46
Counts of Time or Tests Elapsed I10
Days since last service action I10
y Line Code 46: Currently Time or Tests elapsed 
Description Format Range
Line Code 47
Hours or Tests that must pass before 
service action is performed
I10
Number of days that must pass before 
service action is performed
I10
y Line Code 47: Service Due
Description Format Range
Line Code 48
Percentage of Hours or Tests occurred 
for service action to be performed
I10
Percentage of days occurred for service 
action to be performed
I10
y Line Code 48: Elapsed percentage
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes for extended host interface 163
Line Code 49: Rack Position
Example 49_H_2-10<LF>
The first character is either the SLOT_ID letter (A-Z) or a 
whitespace if the slot is not on board. It is then followed 
by [rack number]-[rack position].
Line code 55: Test ID
Example 55_••1_•••2.00_•••3.50<LF>
q Profile and ratio handling: Use ratio number to order 
ratio.
Ratio and individual tests results will be delivered.
The available test no., profile IDs and Ratio IDs can be 
found in Configuration > Test Definitions > ID.
Samples in the instrument can only be diluted. The 
maximum dilution factor is 100. 
If the dilution factor is omitted, the 1:1 factor will be used. 
Description Format Range
Line Code 49
Rack position S10 SlotID_RackNumber-RackPosition
y Line Code 49: Rack Position 
Description Format Range
Line code 55
Test no. I3 1 to 999
Optional If dilution factor is extra information for block 
10
Dilution factor F7 1 to 100
Automatic Rerun 
Dilution factor 
F7 1 to 100
y Line code 55: Test ID
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
164 List of line codes for extended HIF
Line Code 58: Service action result
Example 58_••3_1<LF>
Description Format Range Range description
Line Code 58
Service Action ID I3
Execution result I1 0 none
1 passed
2 aborted
3 failed
4 user action
y Line Code 58: Service action result
ID Description
0 Deproteinize probes
1 Clean probes and splash guard
2 Clean cuvette drum
3 Clean internal water reservoir
4 Clean wash station
5 Clean ext. water reservoir/fluid waste
7 Clean instrument
8 Clean waste box fitting
101 Prime fluid system
102 Roche service
103 Backup database
104 Unlock Action
201 Replace probe B
202 Replace probe C
203 Replace plunger tip dosage pipette B
204 Replace plunger tip dosage pipette C
205 Replace dosage pipette B
206 Replace dosage pipette C
207 Replace wash pipette seal B
208 Replace wash pipette seal C
209 Replace external water reservoir filter
210 Replace ventilation filters
211 Replace Abs. halogen lamp
212 Replace cleaner bottle
301 Electrode service
302 Deproteinize electrodes
303 Etch electrodes
304 Activate electrodes
305 Replace reference (Ref) electrode
y IDs
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes for extended host interface 165
Line Code 60: Calibration due
Example 60_4_25/05/2016<LF>
306 Replace chloride (Cl-) electrode
307 Replace sodium (Na+) electrode
308 Replace potassium(K+) electrode
309 Replace lithium (Li) electrode
310 Prime ISE calibrators
311 Replace ISE module tubing
312 Clean ISE tower automatically
313 Clean ISE tower manually
314 Condition ISE tubing
315 Initialize ISE module
316 ISE Performance Check
401 User action
ID Description
y IDs
Description Format Range Range description
Line Code 60
Calibration due status I1 1 None
2 Next Cassette
3 Next Lot
4 Required
Optional If the calibration is required
Calibration date D1 When is the next calibration due.
y Line Code 60: Calibration due
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
166 List of line codes for extended HIF
Line Code 61: Test Status and blocked status 
Example 61_•3_•1<LF>
If the test status is blocked additional information is 
provided regarding the reason of the impediment.
Description Format Range Range description
Line Code 61
Test Status I2 16 Test disabled
31 Calibration postponed
3 Test blocked
17 OK
Blocked Status I2 Detailed information is provided 
below
y Line Code 61: Test Status and blocked status 
Status code Meaning
0 Okay
1 No fluid in sample cup
2 Reagent not on board
3 Reagent is empty
4 No cassette definition found
5 Diluent not on board
6 Diluent is empty
7 No diluent definition found
8 No calibrator on board
9 Calibrator empty
10 No valid calibration data
11 No calibration defintion available
12 No lot values for calibration available
13 Last calibration is not accepted
14 Control not on board
15 Control empty
16 Control is not valid
17 No control definition available
18 No assigned values for control available
19 Last control not accepted
20 Repeat action not possible
21 Test disabled
22 Error in cuvette handling
23 Test aborted
24 Calculation not possible
25 No ISE Electrode
26 Activation not performed
27 ISE module not ready
28 Error in ISE module
y Status codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes for extended host interface 167
Line Code 62: Calibrator ID 
Example 62_07-1213-2••••••<LF>
Line Code 82: Message Icon
Example 82_•71<LF>
29 Error in Abs. module
30 Error in Fp Module
31 Fatal HW Error
32 Activator not on board
33 Activator is empty
34 No activator definition found
35 Etcher not on board
36 Etcher is empty
37 No etcher definition found
38 Deproteinizer not on board
39 Deproteinizer is empty
40 No deproteinizer definition found
41 ISE module disabled
42 Fp module disabled
43 Cleaner not on board
44 Cleaner empty
45 No cleaner definition available
46 No ISE solution available
47 No ISE solution definition available
48 ISE solution is empty
49 Clot detected in sample
50 Cassette must be mixed first
Status code Meaning
y Status codes
Description Format Range
Line Code 62
Calibrator ID S15 Calibrator ID number
y Line Code 62: Calibrator ID 
Description Format Range
Line Code 82
Message Icon I3 Refer to following table for 
details
y Line Code 82: Message Icon
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
168 List of line codes for extended HIF
Icon Code Description
255 No Icon
9 Analyzer AutoService
4 Analyzer CoverOpen
7 Analyzer FatalError
1 Analyzer Initializing
8 Analyzer PowerDown
10 Analyzer PowerOn
6 Analyzer Running
3 Analyzer Service
0 Analyzer Sleeping
5 Analyzer Standby
2 Analyzer Stopped
140 Cassette Temp Okay
141 Cassette Temp Warning
22 Cleaner Error
20 Cleaner Okay
132 Cleaner Temp Error
130 Cleaner Temp Okay
131 Cleaner Temp Warning
21 Cleaner Warning
32 Cuvette Error
30 Cuvette Okay
31 Cuvette Warning
62 CuvetteWaste Error
60 CuvetteWaste Okay
61 CuvetteWaste Warning
122 ISE Bottle Error
120 ISE Bottle Okay
121 ISE Bottle Warning
72 Message Error
70 Message Okay
71 Message Warning
82 Service Error
80 Service Okay
81 Service Warning
112 Temperature Error
110 Temperature Okay
111 Temperature Warning
52 WasteDrain Error
50 WasteDrain Okay
51 WasteDrain Warning
42 WaterSupply Error
40 WaterSupply Okay
41 WaterSupply Warning
y Icon Codes
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Line codes for extended host interface 169
Line Code 83: Message String
Example 83_Wrong•DB•script.<LF>
q oThe maximum length of each message is 100. If a 
message exceeds this length it will be split in multiple Lines 
83. If the message is shorter also the line will be shorter.
o If a newline character is present in the message string 
this will be displayed as <NL> in order to tell it apart 
from <LF> which is used in the Hif Protocol structure
Line Code 93: System Date Time
Example 93_23/04/2006_22:07:59<LF>
Line Code 94: Serial Number
Example 94_CN123456<LF>
Description Format Range
Line Code 83
String S100
y Line Code 83: Message String
Description Format Range
Line Code 93
Date D1 dd/mm/yyyy
Time T1 hh:mm:ss
y Line Code 93: System Date Time 
Description Format Range
Line Code 94
Serial Number S8 SMP code and Serial 
Number
y Line Code 94: Serial Number
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
170 List of line codes for extended HIF
Line Code 97: System status
Example 97_•5_0_0_0_1_•853_0_•••37.1_•••12.6_•••24.2<LF>
Description Format Range Range description
Line Code 97
System status I2 0 Sleeping
1 Initializing
2 Stopped
3 Service
4 Cover Open
5 Standby
6 Running
7 Fatal Error
8 Power Down
9 Auto Service
10 Power On
Cleaner status I1 0 Ok
1 Warning
2 Error
Cuvette status I1 0 Ok
1 Warning
2 Error
Water supply 
status
I1 0 Ok
1 Warning
2 Error
Cuvette waste 
status
I1 0 Ok
1 Warning
2 Error
Cuvette waste 
count
I4 0..1000
Waste drain 
status
I1 0 Ok
1 Warning
2 Error
Analyzer 
temperature
F7 Temperature in degrees
Cassettes 
temperature
F7 Temperature in degrees
Cleaner 
temperature
F7 Temperature in degrees
y Line Code 97: System status
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Troubleshooting
9 Troubleshooting ....................................................................................................173
 
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
 173
Table of contents
Troubleshooting 9
There are various supporting features that help you 
identify error situations.
In this chapter 9
About troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . 175
Enabling host interface tracing. . . . . . . . . . . . . . . . . . . 176
Viewing the trace file . . . . . . . . . . . . . . . . . . . . . . . . . . . 177
Known host interface issues . . . . . . . . . . . . . . . . . . . . . 178
Sample types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178
Collated results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
174
Table of contents
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1· Host Interface Manual · Version 1.24 
Troubleshooting 175
About troubleshooting
The communication on the host interface can be 
traced/viewed using the 
COBAS INTEGRA 400 plus analyzer or 
Cedex Bio HT Analyzer Message Handler. The Message 
Handler runs in the background as soon as the 
instrument software is active.
q o All Message Handler information is written to the 
...\log\Trace.log file. Please ensure to have the file trace.tra 
(in directory c:\roche\instrument\log\ ) available when you 
call the hot line. Similarly, always attach this file if you mail 
an inquiry.
o The Message Handler does not trace the host 
interface communication when used in the default 
configuration. Thus use the following procedure to 
enable host interface tracing.
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
176 Enabling host interface tracing
Enabling host interface tracing
r To enable host interface tracing
1 Switch to the Message Handler task.
2 From the menu, choose Monitor > Filter.
3 Select the 1023 TRACE_HIF_LINE trace.
4 Choose the OK button.
f The system then records all host interface 
communication. 
5 To make these settings permanent, choose the Save 
button.
I These settings only remain until the next 
shutdown. 
3
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Troubleshooting 177
Viewing the trace file
q The trace file contains only the information which is 
selected by the check boxes.
r To filter and view the HIF 
communication
1 From the menu, choose File > Open trace File.
2 Select Trace.tra.
3 Choose the Open button.
4 Wait until the trace file is loaded completely before 
proceeding to the next step.
5 From the menu, choose Monitor > Filter.
6 Choose the Clear All button.
7 Select item 1023, possibly also items 1020 and 1021.
8 Choose the OK button.
I Do not save this filter setting.
f The display now shows the all traced host interface 
communication. Using this view, you may scroll 
through the complete trace file.
2
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
178 Known host interface issues
Known host interface issues
In this section
Sample types (178)
Collated results (179)
Sample types
• Using a sample specific code instead of “???”, (for 
example HEM for hemolysate) will override any 
instrument and test default sample settings and force 
all tests from the order to be executed on such a 
sample type. This has the same effect as selecting a 
specific sample type on the order entry screen of the 
instrument, instead of automatic, which is the 
defaulting the drop down list. See also details line 
code 53.
• As soon as the sample racks are inserted into the 
instrument, the order number and the sample type 
have to be available to the instrument's database. 
Otherwise the samples are handled as unknown and 
can not be queried by the host. There are two practical 
ways to provide the sample type:
1) Using of sample type defined racks.
2) Using bar codes providing the sample type.
The transmission of the sample type information to the 
LIS computer, however, is independent and can be 
switched off. If bar codes are used for order number 
and sample type, please note that those are sent in 
different data fields and not sent in one string.
u Related topics
• Line code 53: Order ID (96)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Troubleshooting 179
Collated results
• In some cases the preparation of collated results may 
take several minutes and by far exceed the normal 
recommended timeout of 180 seconds.
• Starting with SW V3.4.4. the ratios will be collated with 
other results. This is different from the behavior in SW 
V3.3 where ratios where delivered in a separate block 
as they had automatically a different sample type.
• Collated results selector 04 transmits also blocked and 
invalid test results together with valid ones. Although 
they are clearly flagged as such, this may become an 
issue if the host does not properly handle result flags. 
If you decide to use collated results in spite of 
recommendations, it is better to use 10 03 rather than 
10 04.
u Related topics
• Order entry (33)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
180 Known host interface issues
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Appendices
10 Instrument setup...................................................................................................183
 
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
 183
Table of contents
Instrument setup 10
To ensure proper functioning, the instrument must first be 
correctly installed and its software appropriately 
configured.
In this chapter 10
Setting up the instrument for host communication . . 185
Setting up the instrument for host 
communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185
Preparing an interface cable. . . . . . . . . . . . . . . . . . 186
About the RS-232 cable. . . . . . . . . . . . . . . . . . . 186
About the connector types . . . . . . . . . . . . . . . . 187
About the cable types . . . . . . . . . . . . . . . . . . . . 187
Connecting the interface cable . . . . . . . . . . . . . . . 188
Configuring host communication . . . . . . . . . . . . . . 190
Configuring barcode setup . . . . . . . . . . . . . . . . . . . 192
About the RS-232 serial interface . . . . . . . . . . . . . . . . 195
RS-232 (CCITT V.24) serial interface . . . . . . . . . . . 195
Signal levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
Signal description . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
About the handshake. . . . . . . . . . . . . . . . . . . . . . . . 197
Hardware-handshake. . . . . . . . . . . . . . . . . . . . . 197
Software-handshake . . . . . . . . . . . . . . . . . . . . . 197
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
184
Table of contents
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Instrument setup 185
Setting up the instrument for host 
communication
In this section
Setting up the instrument for host communication (185)
Preparing an interface cable (186)
Connecting the interface cable (188)
Configuring host communication (190)
Configuring barcode setup (192)
Setting up the instrument for host communication
r To set up the instrument for host 
communication
1 Prepare an interface cable and connect the cable to 
the host and the instrument.
2 To enable host interface transmission, choose 
Configuration Processing > Result Output.
• Enable result transmission.
3 To set up interface parameters as needed, choose 
Configuration General > Interface.
• Define interface parameters.
4 To prepare the database set-up, choose 
Configuration Database > Demographics.
• Define patient and order demographics.
5 If you are using barcodes, set up the instrument 
accordingly.
• Enable barcode reading.
• Define the used barcodes and their pattern
• Configure the racks for barcode reading.
6 Load your hostapplication.
• As a first check send an idle block.
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
186 Setting up the instrument for host communication
u Related topics
• Preparing an interface cable (186)
• Connecting the interface cable (188)
• Configuring host communication (190)
• Configuring barcode setup (192)
Preparing an interface cable
In this section
About the RS-232 cable (186)
About the connector types (187)
About the cable types (187)
About the RS-232 cable
We recommend buying an off-the-shelf "null modem" 
RS-232 cable. It will work with the 
COBAS INTEGRA 400 plus analyzer or the 
Cedex Bio HT Analyzer.
The correct pin assignment is defined in the ISO 2110 
standard for 25-pole connectors and in the ISO 4902 
standard for 9-pole connectors.
Pin assignments for RS-232 SW-handshaking
Pin assignments for RS-232 HW-handshaking
Host (DTE) Instrument (DTE)
9 Pin Conn 25 Pin Conn Signal I/O I/O Signal 25 Pin Conn. 9 Pin Conn
3 2 TxD f f RxD 3 2
2 3 RxD g g TxD 2 3
5 7 SigGnd gf gf SigGnd 7 5
4 20 DTR f
Connections according to host system requirements.
6 6 DSR g
7 4 RTS f
8 5 CTS g
1 8 CTS g
y Pin assignments for RS-232 SW-handshaking
Host (DTE) Instrument (DTE)
9 Pin Conn 25 Pin Conn Signal I/O I/O Signal 25 Pin Conn. 9 Pin Conn
3 2 TxD f f RxD 3 2
2 3 RxD g g TxD 2 3
5 7 SigGnd gf gf SigGnd 7 5
y Pin assignments for RS-232 HW-handshaking
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Instrument setup 187
q Please ensure that the suggested wiring matches 
the host system requirements as well.
Some host systems are configured as DCE devices. TxD 
and RxD are then exchanged.
About the connector types
About the cable types
Type To reduce electromagnetic interference, we recommend 
using shielded twisted pair cables having low inductance.
Shield We recommend connecting the cable shield to the SUB-D 
chassis of the interface connector on one side only. 
q NEVER connect the cable shield to the signal 
ground.
Connector housing To prevent electromagnetic interference, we strongly 
recommend using a true metal connector housing, not 
connector housings of plastic.
Length The cable length should not exceed 15 m.
4 20 DTR f f DSR 6 6
6 6 DSR g g DTR 20 4
7 4 RTS f
f DCD 8 1
8 5 CTS g
1 8 DCD g
g RTS 4 7
f CTS 5 8
Host (DTE) Instrument (DTE)
9 Pin Conn 25 Pin Conn Signal I/O I/O Signal 25 Pin Conn. 9 Pin Conn
y Pin assignments for RS-232 HW-handshaking
Instrument Connector type
COBAS INTEGRA 400 plus analyzer 
and Cedex Bio HT Analyzer
SUB-D male type, 9 pin
y Connector types
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
188 Setting up the instrument for host communication
Connecting the interface cable
The COM1 Serial port is dedicated to serve as a host 
interface port. It is a SUB-D male type with 9 pins.
The picture shows the connectors at the right panel of the 
instrument.
COBAS INTEGRA 400 plus analyzer and
Cedex Bio HT Analyzer
Host connector on COBAS INTEGRA 400 plus analyzer 
and Cedex Bio HT Analyzer.
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Instrument setup 189
Data station dc7800
Host connector on COBAS INTEGRA 400 plus analyzer 
and Cedex Bio HT Analyzer on the data station dc7800.
Data station rp5800
Host connector COBAS INTEGRA 400 plus analyzer and 
Cedex Bio HT Analyzer on the data station rp5800.
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
190 Setting up the instrument for host communication
Configuring host communication
Configuration Processing > Result output
Configuration General > Interface
q On the host and the instrument, all interface 
parameters have to be set to the same value.
Parameter Value Description
Sample Selected Sample results are sent to host.
Control Selected Control results are sent to host.
Calibration Selected Calibration results are sent to 
host.
Sample 
Type
Selected Sample type is sent to host.
y Setup for enabling result transmission
Parameter Value range
Baudrate 1200 / 2400 / 4800 / 9600 / 19200 / 
38400 / 57600 / 115200
Data Bits 7 / 8
Stop Bits 1 / 2
Parity None / Even / Odd
Handshake Hardware / Software
Block Check True / False
Line Terminator LF / CR+LF
y Setup of interface parameters
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Instrument setup 191
Configuration Database > Demographics
Configuration Lab > Sample Types
If sample type is enabled in Configuration Processing 
> Result Output, this Sample Type Short Name is 
used on the host interface.
q If sample-type specific racks are not used, this 
Sample Type Short Name has to be included in the 
barcode.
Parameter Value
Patient ID Selected All sample orders have to be 
assigned to an existing patient, 
i.e. prior to downloading 
orders, the relevant patient 
information has to be 
downloaded. The instrument 
generally works with patient 
data.
Not selected The instrument generally 
works without patient data. 
Orders have to be entered 
without patient data.
Free Text 
Fields
Selected The instrument allows defining 
laboratory specific fields. The 
fields and their assigned 
names are shown in the 
patient or order menu. The 
relevant information has to be 
transmitted via the host 
interface.
Not selected The free text fields will not be 
shown in the patient or order 
menu. On the host interface, 
the relevant fields within the 
Patient and Order Entry 
blocks may be left empty.
y Database parameters
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
192 Setting up the instrument for host communication
Configuring barcode setup
Barcode types
q The instrument recognizes the barcode type 
automatically.
Configuration General > Barcode
Use Barcode On
Enable the barcodes in the Use Barcode On section.
Supported barcode types
2 of 5 interleaved
Code 39 (3 of 9 interleaved)
Codabar (Code 2 of 7)
Code 128
IATA 25 (2 of 5 IATA)
INDU 25 (2 of 5 Industrial)
UPC (A, E)
EAN
Pharmacode-EAN Addendum
y Supported barcode types
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Instrument setup 193
Barcode Used
On the host interface, sample identification consists of 
two parts: order number and sample type.
Be aware that they are delivered in two separate fields 
within the order ID, but are combined on the barcode.
If barcode reading is enabled, the order number has to be 
part of the barcode.
Further, the sample type can either be specified by using 
sample-type specific racks, or it also has to be a part of 
the barcode.
q The barcode must not be shorter than the pattern 
you specified.
Example of how to configure the barcode
pattern and the alignment parameter
Pattern item Value
Order number O
Sample Type T
Character not used x
y Barcode pattern
Pattern item Value
Order number 15 characters (alphanumeric)
Sample Type 3 characters (alphanumeric)
y Maximum number of characters
Alignment Effect
Left Left part of barcode is used
Right Right part of barcode is used
y Alignment (only applies if the specified pattern is shorter 
than the barcode used - seeexample below)
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
194 Setting up the instrument for host communication
Configuration Lab > Racks
Barcode reading can be independently enabled / 
disabled for every rack group.
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COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Instrument setup 195
About the RS-232 serial interface
In this section
RS-232 (CCITT V.24) serial interface (195)
Signal levels (195)
Signal description (196)
About the handshake (197)
RS-232 (CCITT V.24) serial interface
The instrument serial interface is based on international 
standards. The relevant standards are EIA RS-232D and 
CCITT V.24.
The RS-232 serial interface is an asynchronous interface 
with ASCII character transfer.
• The characters are transmitted bit-wise.
• The transfer starts with a start bit and ends with one 
or two stop bits.
• There is also a possibility of detecting a bit failure with 
the parity bit.
• The parity bit is selectable as even, odd or none.
Signal levels
The signal levels for data, control and status lines are as 
follows:
• "0" / space / on
• +3 V to +15 V
• "1" / mark / off
• -3 V to -15 V
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
196 About the RS-232 serial interface
Signal description
The following table shows the most commonly used 
signals.
There are about 25 different signals defined in the 
standard of CCITT V.24.
Normally only some of these are used.
CCIT EIA Signal 
name
Description 9 pin 
connector
25 pin 
connector
101 AA PG Protective ground 1
102 AB SG Signal ground or common return 5 7
103 BA TxD Transmitted data 3 2
104 BB RxD Received data 2 3
105 CA TRS Request to send 7 4
106 CB CTS Ready for sending (clear to send) 8 5
107 CC DSR Data set ready 6 6
108/2 CD DTR Data terminal ready 4 20
109 CF DCD Receive line signal detector 1 8
y Signal assignment
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Instrument setup 197
About the handshake
We recommend using SW-handshake (XON, XOFF). Only 
a three wire cable is then necessary.
Nevertheless the HW-handshake conditions must be 
fulfilled. The operating system usually treats HW-
handshake conditions with the highest priority.
In this section
Hardware-handshake (197)
Software-handshake (197)
Hardware-handshake
1. The sender sets its RTS line to active.
This signals the receiver to prepare to receive data 
from the sender station.
2. If the receiver is ready to receive data it sets its DTR 
line to active.
This signals the sender the receiver is ready, 
whereupon the sender starts data transmission.
3. As long as the sender's CTS signal is active, it can 
transmit data.
4. If the receive buffer in the receive station is full (or 
better still, before it is full), the receiver stops the 
transmission by setting the DTR line to inactive.
When the receiver is again ready to receive data, it 
sets DTR to active again.
Software-handshake
Sender After receiving an <XOFF> sign, the send procedure is 
interrupted until receipt of an <XON> sign.
Receiver To interrupt a reception, an <XOFF> sign is sent. Further 
reception can be obtained by sending a <XON>.
<XON>: CHAR(17/11H), DC1
<XOFF>: CHAR(19/13H), DC3
q As soon as the COBAS Instrument software is 
started, the host interface is ready to receive data. No 
initial SW handshake is required.
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
198 About the RS-232 serial interface
Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Index
 Index..........................................................................................................................201
 
 
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
Index
A
Adding tests, 39
ASCII, 55
B
BCS, 22, 26
Block check, 20, 22
Block check sum, 22
Block data, 19
Block header, 19
C
Cables
– RS-232, 186
– types, 187
Codes
– instrument, 57
Collated results, 44
Connectors
– cables, 186
– locations, 188
– types, 187
E
Error codes, 103
F
Feedback, 5
Flags, 103
H
Host query, 40
I
Initilize, 26
Instrument codes, 57
M
Message structure, 18
O
Order entry, 33
P
Patient entry, 31
Pending samples, 40
R
Receive Timeout, 23
Result query
– example, 46
Result requests, 44
– example result query, 46
RS-232, 186
S
SC, 22
Screenshot disclaimer, 4
Sequence counter, 22, 26
Setup, 185
Synchronization, 26
W
Warranty, 4
 
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Roche Diagnostics
COBAS INTEGRA 400 plus analyzer/Cedex Bio HT Analyzer · Software version 3.6/5.1 · Host Interface Manual · Version 1.24 
	COBAS INTEGRA 400 plus analyzer Cedex Bio HT Analyzer
	Publication information
	Table of contents
	Intended use for COBAS INTEGRA 400 plus analyzer
	Intended use for Cedex Bio HT Analyzer
	Symbols and abbreviations
	What is new in publication version 1.24
	What is new in publication version 1.23
	Communication
	About communication
	Communication concept
	Message structure
	Block header
	Block data
	Block check
	Communication control
	Parity check
	Flow control (hardware/software handshake)
	Sequence counter and block check sum
	Check of block and line composition
	Receive timeout
	Error handling
	Host communication handling
	Synchronization and sequence counter use
	About host interface features
	About host interface modes and features
	Patient mode
	Patient entry
	Patient modification
	Patient deletion
	Orders
	Order entry
	Order modification (add tests)
	Order deletion
	Host query mode
	Result requests
	About result requests
	Example of result query
	Example of unspecific result request
	Example of result request with ratio (collated result request)
	Protocol version
	Reference
	General codes
	ASCII code
	Control characters
	Instrument codes
	Block codes
	About block codes
	Idle and synchronization blocks
	Block 00: Idle Block / Sync Block
	Result blocks
	Block 02: Calibration Result
	Block 03: Control Result
	Block 04: Patient Results
	Block 05: Calibration Result with Lot Information
	Block 06: Control Result with Lot Information
	Block 07: Patient Results with Lot Information
	Block 08: Result Request Response
	Block 09: Result Request
	Order blocks
	Block 10: Order Entry
	Block 11: Order Deletion
	Block 19: Order Manipulation Response
	Response to an order entry or test addition
	Response to an order or test deletion
	Patient blocks
	Block 40: Patient Entry
	Block 41: Patient Deletion
	Block 42: Patient Modification
	Block 49: Patient Manipulation Response
	Multi-configuration service blocks
	Block 60: Multi-Configuration Service (MCS) Request (host query)
	Block 61: Slot Configuration
	Block 62: Sample Tube Information
	Block 69: Service Request Response
	Protocol version blocks
	Block 92: Protocol Version Request
	Block 93: Protocol Version Data
	Control messages blocks
	Block 99: Control Message
	Line codes
	About line codes
	List of line codes
	Line code 00: Result Data
	Line code 01: Result Time
	Line code 02: Control ID
	Line code 03: Standard Rates
	Line code 04: Calibration Curve
	Line code 07: ABS Sample Check
	Line code 10: Result Type Selection
	Line code 11: Reagent Lot Information
	Line code 12: Control Lot Information
	Line code 13: Calibration Lot Information
	Line code15: Access Date Time
	Line code 16: Result Indexes
	Line code 40: Service Selection
	Line code 41: Slot State
	Line code 42: Tube Information
	Line code 43: Test State
	Line code 44: Cal/CS State
	Line code 50: Patient ID
	Line code 51: Patient Information
	Line code 52: Special Order Selection
	Line code 53: Order ID
	Line code 54: Order Information
	Line code 55: Test ID
	Line Code 56: Sample name
	Line code 57: Priority Modification
	Line code 96: Error Code
	Line code 98: Protocol Version
	Line code 99: General Error Code
	Messages
	Flags and error codes
	About flags
	Flag X
	Flag CALC (result error)
	Flag QC
	About error codes
	List of line code 96 error codes
	List of line code 99 error codes
	Extended host interface of Cedex Bio HT Analyzer
	Block codes for extended host interface
	About block codes for extended HIF
	Result blocks
	Block 02: Calibration Result
	Block 03: Control Result
	Block 04: Sample Results
	Block 05: Calibration Result with Lot Information
	Block 06: Control Result with Lot Information
	Block 07: Sample Results with Lot Information
	Operation settings blocks
	Block 20: Operation Setting
	Block 21: Operation setting response
	Service Action Blocks
	Block 25: Service Action Execution
	Block 26: Service Action response
	Block 27: Service Action Result Request
	Block 28: Service Action Result Response
	Block 29: Failed Service Action Result Response
	Test status blocks
	Block 45: Test status request
	Block 46: Test Status Response
	Block 47: Failed Test Status Request
	Calibration status blocks
	Block 52: Calibration status request
	Block 54: Calibration Status Response
	Block 57: Failed Calibration Status Request
	Cassette status blocks
	Block 50: Cassette status request
	Block 55: Cassette Status Response
	Block 59: Failed Cassette Status Request
	Multi-configuration service block
	Block 64: Service Action Status
	Message Log blocks
	Block 75: Message Log Request
	Block 76: Message Log Response
	Block 77: Failed Message Log Request
	Sample order status blocks
	Block 80: Order Status Request
	Block 81: Order Status Response
	Block 85: Failed Order Status Request
	System status blocks
	Block 90: System status request
	Block 91: System status response
	Date and time blocks
	Block 94: Date and Time request
	Block 95: Date and Time data
	Serial number blocks
	Block 96: Serial Number Request
	Block 97: Serial Number Response
	Line codes for extended host interface
	About line codes
	List of line codes for extended HIF
	Line Code 06: Absorbance Raw Data
	Line Code 08: FP Raw Data
	Line Code 09: ISE Raw Data
	Line Code 14: Validation Status
	Line Code 17: Test Execution
	Line Code 18: Cassette Information
	Line Code 19: Calibration Information
	Line Code 20: Order ID
	Line Code 21: Control Information
	Line Code 22: Order ID
	Line Code 23: Sample Order Status
	Line Code 24: Message Date Time
	Line Code 25: Test Order Status
	Line Code 26: Dilution Factor
	Line Code 27: Rerun Result
	Line Code 28: Test Status
	Line Code 29: Cassette status
	Line Code 30: Operation selection
	Line Code 31: Calibrator status
	Line Code 32: Service Action selection
	Line Code 33: Control status
	Line Code 36: Cassette definition
	Line Code 37: Tests left
	Line Code 38: Diluent status
	Line Code 39: Cassette Status
	Line code 40: Service Selection
	Line Code 45: Service Action Definition
	Line Code 46: Currently Time or Tests elapsed
	Line Code 47: Service Due
	Line Code 48: Elapsed percentage
	Line Code 49: Rack Position
	Line code 55: Test ID
	Line Code 58: Service action result
	Line Code 60: Calibration due
	Line Code 61: Test Status and blocked status
	Line Code 62: Calibrator ID
	Line Code 82: Message Icon
	Line Code 83: Message String
	Line Code 93: System Date Time
	Line Code 94: Serial Number
	Line Code 97: System status
	Troubleshooting
	Troubleshooting
	About troubleshooting
	Enabling host interface tracing
	Viewing the trace file
	Known host interface issues
	Sample types
	Collated results
	Appendices
	Instrument setup
	Setting up the instrument for host communication
	Setting up the instrument for host communication
	Preparing an interface cable
	About the RS-232 cable
	About the connector types
	About the cable types
	Connecting the interface cable
	Configuring host communication
	Configuring barcode setup
	About the RS-232 serial interface
	RS-232 (CCITT V.24) serial interface
	Signal levels
	Signal description
	About the handshake
	Hardware-handshake
	Software-handshake
	Index
	Index