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TRICHOME TRIVIA 10 Topics to Test Your Weed Knowledge in 2021 Question 1 What is the primary purpose of cannabis terpenes? a. Attract pollinators b. Capture pollen c. Protect the plant d. Produce THC 2 Answer C. Protect the plant There are over 20,000 characterized terpenes in the plant kingdom and their primary function is to protect plants! Every plant we can smell produces aromatic compounds like terpenes. The scent of terpenes help deter herbivores, insects, fungi, and other plant predators. The acidic, spicy, and bitter flavors of terpenes also help prevent many animals from eating plants. There have been hundreds of terpenes identified throughout the various cannabis varieties. Considering cannabis shares dominant terpenes with other common plants, strain names often reference fruits like lemons, tangerines, blueberries, and strawberries. Grapefruit Haze, Tangerine Dream, and Lemon Skunk as examples, smell incredibly similar to the fruits they are named after. The limonene, pinene, and other terpenes found in lemon, are also found in Lemon Skunk and other cannabis varieties that have strong citrus aromatics. Moreover, terpenes are medicinally useful and have been proven to have analgesic, antibacterial, anti-carcinogenic, bronchodilation, anticonvulsant, antifungal, anti-anxiety, and many other benefits! 3 4 The following chart features six of the most common cannabis terpenes. 5 Question 2 Approximately how old is the word marijuana? a. 60 years b. 100 years c. 400 years d. 3,000 years Answer C. 400 years old First uses of the word marijuana date back to approximately 400 years ago. The origin of this word is a perfect example of misinformation, and is nearly as misunderstood as the plant itself. In the video linked below, Professor Santiago Guerra explains the truth behind the word marijuana and it’s amazing history. 6 Watch Professor Santiago explain the truth about the word marijuana! ← https://blog.trichomeinstitute.com/the-history-of-the-word-marijuana https://blog.trichomeinstitute.com/the-history-of-the-word-marijuana Question 3 Which trichome is the ripest? A. B. C. Answer: Image B The very reason people consume cannabis stems from the chemistry within the glandular trichomes. Therefore, if the trichomes are not healthy and mature, the flower will lack potency, psychotropic effects, and medicinal potential. When evaluating trichome quality, use a magnifier or jeweler’s loupe to confirm if the majority of the trichomes are clear (premature), milky (mature), or amber (overripe). Flower with an abundance of milky trichomes, few clear or amber trichomes, and minimal to no broken glandular heads is ideal. Sometimes you can find all three of these ripeness levels within the same flower, which can be considered poor genetics. Ultimately, trichome health and ripeness determines the potency and psychotropic effects of the cannabinoids and the terpenes. 8 9 Question 4 What’s the difference between a topical and a transdermal cannabis product? a. Transdermals stay on top of the skin b. Topicals penetrate the epidermis c. Transdermals contain active ingredients that enter the bloodstream d. Topicals smell better than transdermals Answer C. Transdermals contain active ingredients that enter the bloodstream Transdermal administration requires a patch or topical solution containing special permeation agents that carry cannabinoids through the skin and into the bloodstream. Transdermal administration differs from topicals, which do not penetrate past the upper layer of epidermis or enter the bloodstream and only provide localized relief. A key component that separates transdermal patches from other types of cannabis products is that they can continuously dose cannabinoids over an extended period of time. Transdermals are a great option for people who cannot dose throughout the day or night, have continuous pain, are unable to swallow, or need alternative delivery methods. Transdermal patches are also the only long-acting treatment available for people who do not respond positively to edibles or have gastrointestinal or digestive issues. 10 Topicals: Despite some similarities, topical cannabis products differ greatly from transdermal products because they do not penetrate the epidermis and are not absorbed into the bloodstream. So remember, topicals go on top! Cannabis infused topical lotions and creams are easily spread over large areas of skin and treat a variety of ailments. Topicals are used for their anti-fungal, antiseptic, analgesic, and anti-inflammatory properties, and are great for skin issues like burns, rashes, bruises, acne, scars, eczema, athletes foot, arthritis, and more. In theory, topicals should not show up on drug tests because they do not enter into the bloodstream like a transdermal product. Therefore, they won’t make you feel high, but this isn’t always the case. If a topical product has an ingredient like olive oil, which contains a permeation agent, the topical may act as a transdermal and could enter your bloodstream and show up on a drug test. 11 12 Question 5 Which flower has powdery mildew? A. B. C. Answer: Image A Powdery Mildew (PM) is an extremely common mold that can be found in many gardens. It appears as a white powdery substance that clumps in spots on leaves before spreading to the rest of the plant. PM does not produce any odor and is not systemic. Certain cannabis varieties are more susceptible to PM than others, but the regulation of temperature and humidity is ultimately what prevents PM from infecting plants. PM spores are easily spread by visiting contaminated grows or from a dispensary with traffic from dozens of home growers. Growers often ask themselves, “Where on earth is this mildew coming from?” The answer: from the growers themselves. 13 14 Jar rot mold develops when fresh flower is improperly dried and cured, or is put into a jar with insufficient air circulation and excess humidity, restricting the product from “breathing” as it dries out or cures. Moisture combined with lack of circulation promotes rot on most plant kingdom organisms, and cannabis is no exception. Jar rot is the same fast-growing, light grey mold found on strawberries that are left in the refrigerator for too long. If jar rot is fresh, it will smell slightly damp and a grey fuzzy mold will be present. If the flower has jar rot and then dries out, mold residue can be seen faintly, but will not have an odor. 15 Botrytis, also known as “bud rot,” is the silent killer of cannabis. Botrytis is a systemic mold that can lie dormant and undetectable until specific environmental conditions activate it. High humidity and a lack of air circulation create the perfect environment for botrytis spores to jump-start their life cycles. Botrytis spores can spread like wildfire through an entire garden, quickly developing and infecting plants. The primary indicators of botrytis are discoloration and off-putting odors. If there is reddish-brown or light grey fuzzy mold on the bracts and around the stem, or colors such as grey, red, tan, or brown close to the base of the flower, it’s most likely botrytis. Heavy botrytis has a very specific odor that is simultaneously semi-sweet and acrid, and can actually upset your stomach upon catching a good whiff. 16 Question 6 Which of the following images is the cannabis flower? A. B. C. D. Answer: Image C Inflorescence is defined as the complete flower head of a plant, including stems, stalks, bracts, and flowers. Simply put, inflorescence is the arrangement of the flowers on a plant. With cannabis, what is often referred to as the cola, bud, or flower, is actually the inflorescence. Cannabis inflorescences are composed of dozens or even hundreds of individual flowers, or florets, which are usually referred to as thecalyx. However, what has been called the calyx for decades is actually the entire flower. The largest visible part of the flower is the bract, which encloses the entire calyx and reproductive anatomy, and has two pistils protruding from the top. To put this into perspective, think about the last time you closely examined a sunflower. Did you take the time to notice each and every tiny, little flower of the sunflower inflorescence? Or were you salivating for salty, crunchy sunflower seeds to enjoy at a ballgame instead? If you look again closely, you will notice that what we call a “sunflower” is actually hundreds of tiny little flowers, each containing their own reproductive anatomies and producing their own seeds. Sunflower and cannabis plants both have in common their own types of inflorescence and are similar in how we refer to them as a singular form, even though we are actually talking about many, many flowers. 18 19 Question 7 What insect leaves freckles on the underside of a cannabis leaf? a. Aphids b. Spider mites c. Fungus gnats d. Ladybugs Answer B. Spider Mites Insect infestations are common with cannabis cultivation. A variety of insects—including aphids, spider mites, thrips, fungus gnats, white fly, and many more—find their way into gardens, and if left untreated can devastate a grow. Insects feed off the cannabis plant or infest the growing medium, causing stunted growth and a slew of other issues. Insects injure the flowers, leaves, branches, and roots by feeding on them and then leaving residual byproducts such as carcasses, fecal matter, eggs, webs, and exoskeletons. Some insects that infest flowers are too small to be seen by the naked eye, which is why a magnifier is often necessary for proper detection. 20 Answer B. Trichome 2 | Milky Freckle-like blemishes on leaves can be spotted from several feet away and result from spider mites eating the underside of the leaf. Always look for small, discolored spots on the leaves and trim. If a plant displays these spots, it should be further examined for spider mites and other insects. 21 22 Question 8 Which of the following images is considered wax? B. A. C. Answer: Image B When discussing cannabis extracts in a retail context, there are many names you may hear. It is common for the terms concentrate, extract, resin, hash oil, and a few others to be used interchangeably. What they refer to is a concentrated form of active ingredients, such as cannabinoids and terpenes, which are extracted from the plant material. There are two primary categories of cannabis concentrates: homogeneous and non-homogeneous. Homogeneous concentrates are uniform in their structure and mostly opaque. Non-homogeneous concentrates are unevenly distributed in their structures and can be opaque in some areas and translucent in others. Here is the biggest difference between these two categories: If you were to take two 1g samples out of a homogeneous extract, the milligrams of active ingredients of each sample would be nearly identical. However, if you were to take two 1g samples out of a nonhomogeneous extract, the milligrams of active ingredients would vary significantly. 23 Wax is an overarching term for homogeneous concentrate products, but there are different names that fall under wax depending on the consistency, texture, or simply the branding of the product. Think of wax like scrambled eggs. There are a bunch of ways to cook scrambled eggs, but the final product is a blend of the yolk and white cooked together. You can beat the eggs in a bowl or slowly stir them while they are cooking. You can scramble eggs on high heat and they will be spongy. On low heat and they will be delicate and creamy. You can cook eggs with spray on oil, fancy truffle oil, margarine, butter, or use no fat with a really good nonstick pan. No matter how you make scrambled eggs they are still scrambled at the end. The terpene content of wax concentrates will determine the texture and aroma. Wax products with a higher terpene profile will appear and feel greasy or wet, and that’s a good thing, but they will still be properly homogenized. Wax Product Examples 24 Budder Crumble Honeycomb Sugar Question 9 Which extraction method requires -30°c to -50°c temperatures? a. Ethanol b. CO2 c. Bubble Hash @deltaseparations Answer A. Ethanol Ethanol extraction is a very common method used in many industrial applications because it’s largely regarded as safe for human consumption and is an excellent solvent, especially for cannabis. The purity of ethanol alcohol is crucial, which is why 190-200 proof ethanol is required for proper extraction. Ethanol excels at extracting numerous compounds from plants. However, with ethanol extraction there are some unwanted compounds that a cannabis extractor would not want in their extract, such as chlorophyll, sugars, and moisture. This is why the temperature of the ethanol important is important for extraction. The warmer the temperature of the ethanol, the more undesirable compounds there will be in the extraction. Therefore, ethanol is cooled to -30°C to -50°C, which is great for extracting cannabinoids and terpenes, but not other compounds that will lessen the purity of a cannabis concentrate product. 26 In order to maintain the temperature of the ethanol during the extraction process, many extractors freeze their plant material prior to extraction and use special jackets to insulate the extraction equipment. Ethanol Extractor @deltaseparations Ethanol extraction equipment can vary but you will typically see a centrifuge device that functions similarly to your washing machine at home. A mesh bag filled with finely ground cannabis is placed into a machine that washes the plant material repeatedly with ethanol alcohol as it spins. This allows the alcohol to dissolve as much of the plant resin as possible. One of the most common pieces of equipment used for ethanol solvent recovery is a rotary evaporator, or rotovap. The extract is put under vacuum conditions in the rotovap and continuously spun at a warm enough temperature to evaporate the ethanol but not the active ingredients. The spinning process ensures that the heat is distributed evenly throughout the extract This process is a form of distillation which separates the purified ethanol so that it is able to be reused in the future. Falling film evaporators are used in extraction labs with much higher volumes. 27 Not all of the ethanol is recovered, which is why lab tests are required to determine purity and safety of the final product. No matter how great the solvent recovery process is, there will always be some small percentage loss of ethanol that is left in your crude oil, which is then further refined depending on the desired final product. Falling Film Evaporator @deltaseparations 28 Question 10 Which of the following edibles can also be a sublingual? A. C. B. Answer A. Suckers or Lollipops Most people think of the stomach when it comes to an edible high, but what really makes the magic happen is the liver. When decarboxylated or activated D9-THC is digested by the stomach, it is then sent to the liver. The liver metabolizes D9-THC into 11-Hydroxy-THC and this new form of THC (which isn’t found naturally in the cannabis plant) is a lot stronger and more intoxicating than D9-THC, the primary compound that makes you feel high from inhaling marijuana. When you are enjoying an edible like a lollipop, mint, lozenge, or other food product that you would keep in your mouth until it is mostly dissolved, it is also acting as a sublingual. As your saliva dissolves a lollipop, for example, the active cannabinoids are mixed with your saliva, slowly being pulled into your bloodstream via capillaries in your mouth. The longer you hold yoursaliva without swallowing, the more cannabinoids will be sublingually delivered. Sublingually delivered THC bypasses your liver, which prevents the metabolism of 11-Hydroxy-THC. Since you are eventually going to swallow your saliva that’s filled with delicious flavor and cannabinoids, you will then be putting your liver to work in form of a traditional edible. 29 In order for a sublingual product to be effectively delivered into your bloodstream, it must be held in your mouth for 1-3 minutes. Since most people aren’t holding their saliva in their mouth for 1-3 minutes while enjoying a lollipop, the sublingual delivery of cannabinoids will be low. However, once you’re done with your lollipop, you’re still going to have a different ratio of D9:11-Hydroxy-THC in your system in comparison to eating a normal edible. This explains why some people feel a different sensation from eating different edible products, like a brownie versus a lollipop. Try this! Purchase two identical lollipops from a dispensary in your legal state. On one night eat the lollipop immediately by chewing and swallowing it like any other edible. On another night take your time with the second lollipop by sucking on it and try not to swallow as often as you normally would. Take notes on how you feel from each high and see if you notice any difference between the two experiences. Be sure that the setting, time of the day, your mood, and time from last meal, are all the same to minimize variables that could alter your experiences. 30 Well done! If you made it here, you deserve a high-five for furthering your cannabis knowledge. Trichome Institute is grateful for people like you who are not only advocates for cannabis, but advocates for cannabis education. We hope you continue to include us in your cannabis education journey. You’re here to learn, we’re here to guide you. #WeedBetter Copyright © 2021 TrichomeInstitute.com http://www.instagram.com/trichome.institute http://www.facebook.com/trichomeinstitute https://www.linkedin.com/company/trichome-institute