MÉTODO DE KATO-KATZ - LABORATÓRIO (PRÉ-TESTE)

Parasitologia Clínica

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ESTÁCIO

Ludmila Dos Santos Costa

em 23/08/2023

Questões resolvidas

No laboratório virtual, before accessing the workbench with all personal protective equipment (PPE), it is extremely necessary to sanitize your hands. Point out which PPE you will use in this practice, after the sanitization procedure. Incorrect answer. A. Lab coat, gloves, mask, and 70% alcohol. 70% alcohol is not considered PPE. You got it right! B. Lab coat, gloves, goggles, and mask. All of these are considered PPE and are necessary to perform the experiment according to the Kato-Katz method protocol. Incorrect answer. C. Lab coat, gloves, goggles, and beaker with sodium hypochlorite. Beaker with hypochlorite is not PPE. It is used to sanitize the materials that will be discarded.

a) Lab coat, gloves, mask, and 70% alcohol.
b) Lab coat, gloves, goggles, and mask.
c) Lab coat, gloves, goggles, and beaker with sodium hypochlorite.

The Kato-Katz method is a widely used tool to monitor and assess the prevalence and geographical distribution of soil-transmitted helminths and Schistosoma mansoni infections. Indicate the limitations of the method. Incorrect answer. A. The methodology is considered simple and can be easily reproduced on a large scale. The method has weak sensitivity to detect mild infections, and there is substantial variation in readings (resulting from daily fluctuations in egg excretion, uneven distribution of eggs in a single stool sample, and variation in technicians' capacity). You got it right! B. The method has weak sensitivity to detect mild infections, and there is substantial variation in readings (resulting from daily fluctuations in egg excretion, uneven distribution of eggs in a single stool sample, and variation in technicians' capacity). The method has low sensitivity to detect mild infections, and there is substantial variation in readings (resulting from daily fluctuations in egg excretion, uneven distribution of eggs in a single stool sample, and variation in technicians' capacity). Incorrect answer. C. The method can be taught to laboratory microscopists with relative complexity. The method can be taught to laboratory microscopists with relative ease.

a) The methodology is considered simple and can be easily reproduced on a large scale. The method has weak sensitivity to detect mild infections, and there is substantial variation in readings (resulting from daily fluctuations in egg excretion, uneven distribution of eggs in a single stool sample, and variation in technicians' capacity).
b) The method has weak sensitivity to detect mild infections, and there is substantial variation in readings (resulting from daily fluctuations in egg excretion, uneven distribution of eggs in a single stool sample, and variation in technicians' capacity).
c) The method can be taught to laboratory microscopists with relative ease.

In the experiment for the execution of the Kato-Katz method, a portion of the stool is collected with a small spatula and placed on a quantifying plate, allowing a standardized amount of stool to be examined under the microscope and the eggs to be counted. In this context, analyze the following statements: I. The first step of the slide preparation technique consists of passing a small amount of the stool sample through a nylon screen to remove large particles. II. One step in the preparation of slides by the Kato-Katz method is the transfer of the sieved portion of the sample to the central hole of a quantifying plate on a glass slide and the placement of a cellophane cover slip over the sample to spread it. III. A 60-minute incubation period is necessary before the slide can be analyzed under a microscope for the detection and counting of recovered eggs. Which statements are correct? Incorrect answer. A. Only I. Statement I is correct, but it is not the only one. Incorrect answer. B. Only II. Statement II is correct, but it is not the only one. You got it right! C. I and II. The statements are correct as they present the steps to perform the Kato-Katz method experiment.

a) Only I.
b) Only II.
c) I and II.

To evaluate the quality of a diagnostic test, important variables such as sensitivity and specificity are considered to measure and identify the diagnostic accuracy of the method. Regarding the Kato-Katz method, it is safe to say that: Incorrect answer. A. It has high specificity and high sensitivity. It has low sensitivity because egg counting can be inaccurate, as it depends on the technician who will evaluate it. In addition, a low parasite load may not be detected by the technique.

a) It has high specificity and high sensitivity.
b) It has low sensitivity because egg counting can be inaccurate, as it depends on the technician who will evaluate it. In addition, a low parasite load may not be detected by the technique.

Indicate the alternatives that represent the methodologies that are indicated as complementary to the Kato-Katz method in order to reduce the possibility of false-negative diagnoses.

I. Métodos imunológicos.
II. Contagem de ovos de helmintos por grama de amostra de fezes.
III. Métodos baseados em biologia celular.
IV. Métodos baseados em biologia molecular.

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Questões resolvidas

No laboratório virtual, before accessing the workbench with all personal protective equipment (PPE), it is extremely necessary to sanitize your hands. Point out which PPE you will use in this practice, after the sanitization procedure. Incorrect answer. A. Lab coat, gloves, mask, and 70% alcohol. 70% alcohol is not considered PPE. You got it right! B. Lab coat, gloves, goggles, and mask. All of these are considered PPE and are necessary to perform the experiment according to the Kato-Katz method protocol. Incorrect answer. C. Lab coat, gloves, goggles, and beaker with sodium hypochlorite. Beaker with hypochlorite is not PPE. It is used to sanitize the materials that will be discarded.

a) Lab coat, gloves, mask, and 70% alcohol.
b) Lab coat, gloves, goggles, and mask.
c) Lab coat, gloves, goggles, and beaker with sodium hypochlorite.

The Kato-Katz method is a widely used tool to monitor and assess the prevalence and geographical distribution of soil-transmitted helminths and Schistosoma mansoni infections. Indicate the limitations of the method. Incorrect answer. A. The methodology is considered simple and can be easily reproduced on a large scale. The method has weak sensitivity to detect mild infections, and there is substantial variation in readings (resulting from daily fluctuations in egg excretion, uneven distribution of eggs in a single stool sample, and variation in technicians' capacity). You got it right! B. The method has weak sensitivity to detect mild infections, and there is substantial variation in readings (resulting from daily fluctuations in egg excretion, uneven distribution of eggs in a single stool sample, and variation in technicians' capacity). The method has low sensitivity to detect mild infections, and there is substantial variation in readings (resulting from daily fluctuations in egg excretion, uneven distribution of eggs in a single stool sample, and variation in technicians' capacity). Incorrect answer. C. The method can be taught to laboratory microscopists with relative complexity. The method can be taught to laboratory microscopists with relative ease.

a) The methodology is considered simple and can be easily reproduced on a large scale. The method has weak sensitivity to detect mild infections, and there is substantial variation in readings (resulting from daily fluctuations in egg excretion, uneven distribution of eggs in a single stool sample, and variation in technicians' capacity).
b) The method has weak sensitivity to detect mild infections, and there is substantial variation in readings (resulting from daily fluctuations in egg excretion, uneven distribution of eggs in a single stool sample, and variation in technicians' capacity).
c) The method can be taught to laboratory microscopists with relative ease.

In the experiment for the execution of the Kato-Katz method, a portion of the stool is collected with a small spatula and placed on a quantifying plate, allowing a standardized amount of stool to be examined under the microscope and the eggs to be counted. In this context, analyze the following statements: I. The first step of the slide preparation technique consists of passing a small amount of the stool sample through a nylon screen to remove large particles. II. One step in the preparation of slides by the Kato-Katz method is the transfer of the sieved portion of the sample to the central hole of a quantifying plate on a glass slide and the placement of a cellophane cover slip over the sample to spread it. III. A 60-minute incubation period is necessary before the slide can be analyzed under a microscope for the detection and counting of recovered eggs. Which statements are correct? Incorrect answer. A. Only I. Statement I is correct, but it is not the only one. Incorrect answer. B. Only II. Statement II is correct, but it is not the only one. You got it right! C. I and II. The statements are correct as they present the steps to perform the Kato-Katz method experiment.

a) Only I.
b) Only II.
c) I and II.

To evaluate the quality of a diagnostic test, important variables such as sensitivity and specificity are considered to measure and identify the diagnostic accuracy of the method. Regarding the Kato-Katz method, it is safe to say that: Incorrect answer. A. It has high specificity and high sensitivity. It has low sensitivity because egg counting can be inaccurate, as it depends on the technician who will evaluate it. In addition, a low parasite load may not be detected by the technique.

a) It has high specificity and high sensitivity.
b) It has low sensitivity because egg counting can be inaccurate, as it depends on the technician who will evaluate it. In addition, a low parasite load may not be detected by the technique.

Indicate the alternatives that represent the methodologies that are indicated as complementary to the Kato-Katz method in order to reduce the possibility of false-negative diagnoses.

I. Métodos imunológicos.
II. Contagem de ovos de helmintos por grama de amostra de fezes.
III. Métodos baseados em biologia celular.
IV. Métodos baseados em biologia molecular.

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Método Direto a Fresco PRÉ E PÓS TESTE

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MÉTODO DE HOFFMANN PRÉ E PÓS TESTE

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OAL 04 Parasitologia Clínica

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Perguntas dessa disciplina

Os métodos eletroquímicos se baseiam nas propriedades elétricas de uma solução contendo o analito, quando esta laz parte de uma célula eletroquímica.

ANHANGUERA

Preencha corretamente a lacuna do texto abaixo: Uma das medidas preventivas de controle que deve ser adotada é a relativa ao meio Resposta 1 Quest...

UNIASSELVI IERGS

O exame citopatológico cérvico-vaginal é o método de eleição para o rastreamento do câncer de colo do útero em larga escala, devido à sua relação c...

UNAMA

Questão 1 PARASITOLOGIA CLINICA Código da questão: 292493 O exame parasitológico de fezes é um procedimento fundamental para O diagnóstico das para...

UNINASSAU

Questão 11 CONTROLE DE QUALIDADE MICROBIOLOGICO Código da questão: 265813 Leia o trecho a seguir: "A qualidade microbiológica exigida de um fármaco...

UNINASSAU